Regulation of the Macrophage Vacuolar ATPase and Phagosome-Lysosome Fusion by Histoplasma capsulatum

• Published in: The Journal of immunology (1950) Vol. 162; no. 10; pp. 6148 - 6154
• Main Authors: Strasser, Jane E, Newman, Simon L, Ciraolo, Georgianne M, Morris, Randal E, Howell, Michael L, Dean, Gary E
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.05.1999
• Subjects: Animals; Histoplasma - immunology; Histoplasma capsulatum; Lysosomes - enzymology; Lysosomes - microbiology; Macrophages - immunology; Macrophages - microbiology; Membrane Fusion; Mice; Mice, Inbred BALB C; Organelles - enzymology; Organelles - microbiology; Phagosomes - enzymology; Phagosomes - microbiology; Proton-Translocating ATPases - biosynthesis; Vacuolar Proton-Translocating ATPases

Histoplasma capsulatum (Hc) maintains a phagosomal pH of about 6.5. This strategy allows Hc to obtain iron from transferrin, and minimize the activity of macrophage (Mo) lysosomal hydrolases. To determine the mechanism of pH regulation, we evaluated the function of the vacuolar ATPase (V-ATPase) in...