Clinical and immunological responses in sheep after inoculation with Himar1-transformed Anaplasma phagocytophilum and subsequent challenge with a virulent strain of the bacterium

• Published in: Veterinary immunology and immunopathology Vol. 231; p. 110165
• Main Authors: Eskeland, Sveinung, Stuen, Snorre, Munderloh, Ulrike G., Barbet, Anthony, Crosby, Liliana, Lybeck, Kari, Wilhelmsson, Peter, Lindgren, Per-Eric, Makvandi-Nejad, Shokouh, Tollefsen, Stig, Granquist, Erik G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021
• Subjects: acaricides; Anaplasma phagocytophilum; animal welfare; bacteremia; bacteria; cell lines; disease control; genetic transformation; Himar1-mutants; humoral immunity; immunogenicity; immunopathology; Norway; nucleotide sequences; Peripheral blood mononuclear cells; Serological response; tick-borne diseases; ticks; Vaccination; vaccines; virulence; virulent strains; Norway; Anaplasma phagocytophilum; Peripheral blood mononuclear cells; Himar1-mutants; Vaccination; Serological response

•Himar1-transposon was inserted into a virulent strain of Anaplasma phagocytophilum.•The virulence of the mutated strains was unchanged when inoculated in sheep.•Partial clinical immunity against virulent challenge was observed in sheep.•CD4+:CD8+ ratio after challenge was not skewed to a CD8+ respo...