Rationally engineered double substituted variants of Yarrowia lipolytica lipase with enhanced activity coupled with highly inverted enantioselectivity towards 2-bromo phenyl acetic acid esters - Cambon, Emmanuelle; Piamtongkam, Rungtiwa; Bordes, Florence; Duquesne, Sophie; André, Isabelle; Marty, Alain (Biotechnology and bioengineering, 2010)
Keywords: Acetates - metabolism; Acetic acid; Amino Acid Substitution; Amino acids; Bioengineering; Biotechnology; enantioselectivity; Enzymatic activity; enzyme evolution; Enzyme kinetics; Esters; Fungal Proteins - genetics; Fungal Proteins - metabolism; Hydrocarbons, Brominated - metabolism; Life Sciences; lipase; Lipase - genetics; Lipase - metabolism; Molecular Dynamics Simulation; Mutagenesis; Mutagenesis, Site-Directed; Mutation; Pharmaceutical industry; Phenylacetates - metabolism; Protein folding; rational engineering; Stereoisomerism; Substrate Specificity; Yarrowia - enzymology; Yarrowia lipolytica