Single-cell RNA sequencing reveals the cellular heterogeneity of aneurysmal infrarenal abdominal aorta

• Published in: Cardiovascular research Vol. 117; no. 5; pp. 1402 - 1416
• Main Authors: Zhao, Guizhen, Lu, Haocheng, Chang, Ziyi, Zhao, Yang, Zhu, Tianqing, Chang, Lin, Guo, Yanhong, Garcia-Barrio, Minerva T, Chen, Y Eugene, Zhang, Jifeng
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 23.04.2021
• Subjects: Animals; Aorta, Abdominal - metabolism; Aorta, Abdominal - pathology; Aortic Aneurysm, Abdominal - chemically induced; Aortic Aneurysm, Abdominal - genetics; Aortic Aneurysm, Abdominal - metabolism; Aortic Aneurysm, Abdominal - pathology; Cell Lineage; Cluster Analysis; Disease Models, Animal; Editor's Choice; Gene Expression Profiling; Macrophages - metabolism; Macrophages - pathology; Mice; Mice, Inbred C57BL; Monocytes - metabolism; Monocytes - pathology; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - pathology; Myocytes, Smooth Muscle - metabolism; Myocytes, Smooth Muscle - pathology; Original; Pancreatic Elastase; Phenotype; RNA-Seq; Single-Cell Analysis; Transcriptome; Vascular smooth muscle cell; Lineage heterogeneity; Single-cell RNA sequencing; Abdominal aortic aneurysm; Macrophage
• DOI: 10.1093/cvr/cvaa214
• ISSN: 0008-6363; 1755-3245

Abstract Aims The artery contains numerous cell types which contribute to multiple vascular diseases. However, the heterogeneity and cellular responses of these vascular cells during abdominal aortic aneurysm (AAA) progression have not been well characterized. Methods and results Single-cell RNA sequencing was performed on the infrarenal abdominal aortas (IAAs) from C57BL/6J mice at Days 7 and 14 post-sham or peri-adventitial elastase-induced AAA. Unbiased clustering analysis of the transcriptional profiles from >4500 aortic cells identified 17 clusters representing nine-cell lineages, encompassing vascular smooth muscle cells (VSMCs), fibroblasts, endothelial cells, immune cells (macrophages, T cells, B cells, and dendritic cells), and two types of rare cells, including neural cells and erythrocyte cells. Seurat clustering analysis identified four smooth muscle cell (SMC) subpopulations and five monocyte/macrophage subpopulations, with distinct transcriptional profiles. During AAA progression, three major SMC subpopulations were proportionally decreased, whereas the small subpopulation was increased, accompanied with down-regulation of SMC contractile markers and up-regulation of pro-inflammatory genes. Another AAA-associated cellular response is immune cell expansion, particularly monocytes/macrophages. Elastase exposure induced significant expansion and activation of aortic resident macrophages, blood-derived monocytes and inflammatory macrophages. We also identified increased blood-derived reparative macrophages expressing anti-inflammatory cytokines suggesting that resolution of inflammation and vascular repair also persist during AAA progression. Conclusion Our data identify AAA disease-relevant transcriptional signatures of vascular cells in the IAA. Furthermore, we characterize the heterogeneity and cellular responses of VSMCs and monocytes/macrophages during AAA progression, which provide insights into their function and the regulation of AAA onset and progression. Graphical Abstract