Exogenous Cytokine-Free Differentiation of Human Pluripotent Stem Cells into Classical Brown Adipocytes

• Published in: Cells (Basel, Switzerland) Vol. 8; no. 4; p. 373
• Main Authors: Oka, Masako, Kobayashi, Norihiko, Matsumura, Kazunori, Nishio, Miwako, Saeki, Kumiko
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.04.2019; MDPI
• Subjects: Adipocytes; Adipocytes, Brown - cytology; Adipocytes, Brown - metabolism; Adipocytes, Brown - physiology; Adrenergic receptors; Animals; Beta cells; brown adipocyte; Cell culture; Cell Culture Techniques - methods; Cell differentiation; Cell Differentiation - physiology; Cell size; Cells, Cultured; cytokine-free; Cytokines; Cytokines - pharmacology; Fibroblasts; Human Embryonic Stem Cells - cytology; Human Embryonic Stem Cells - metabolism; human pluripotent stem cells; Humans; Induced Pluripotent Stem Cells - cytology; Insulin; Insulin secretion; Insulin-Secreting Cells - cytology; Lipids; Medicine; Metabolism; Mice; Mimicry; Mitochondria; Myoblasts; Obesity; Pancreas; Paracrine signalling; Pluripotency; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; size-controlled spheroids; Spheroids; Spheroids, Cellular - metabolism; Stem cell transplantation; Stem cells; Thermogenesis; United States > US; Japan; cytokine-free; human pluripotent stem cells; brown adipocyte; size-controlled spheroids

We previously established a method for a directed differentiation of human pluripotent stem cells into classical brown adipocytes (BA) by forming aggregates via massive floating culture in the presence of a specific cytokine cocktail. However, use of recombinant cytokines requires significant cost....