A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA

• Published in: Applied and environmental microbiology Vol. 84; no. 15
• Main Authors: Nakamura, Yasuyuki, Nishi, Teruyuki, Noguchi, Risa, Ito, Yoichiro, Watanabe, Toru, Nishiyama, Tozo, Aikawa, Shimpei, Hasunuma, Tomohisa, Ishii, Jun, Okubo, Yuji, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.2018
• Subjects: Autonomously replicating sequence; Biotechnology; Chromosome 2; Chromosomes; Data processing; Deoxyribonucleic acid; DNA; Gene expression; Gene sequencing; Genetic engineering; Genetic transformation; Genomes; Harbor facilities; Inverted repeat; Mass production; Nucleotide sequence; Pichia pastoris; Plasmids; Proteins; Replication; Ribonucleic acid; RNA; Yeast; inverted repeat; centromere; plasmid retention; Pichia pastoris; autonomously replicating plasmid

Secretory production of recombinant proteins is the most important application of the methylotrophic yeast Pichia pastoris , a species that permits mass production of heterologous proteins. To date, the genetic engineering of P. pastoris has relied largely on integrative vectors due to the lack of u...