Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy

Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and to be applied in the field of regenerative medicine. The study of the cell-matrix interaction is important for the understanding of cell attachment, proliferation, and migration, as well as for the improvement of th...

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Published inCells (Basel, Switzerland) Vol. 11; no. 24; p. 4137
Main Authors Tikhonova, Tatiana N., Kolmogorov, Vasilii S., Timoshenko, Roman V., Vaneev, Alexander N., Cohen-Gerassi, Dana, Osminkina, Liubov A., Gorelkin, Petr V., Erofeev, Alexander S., Sysoev, Nikolay N., Adler-Abramovich, Lihi, Shirshin, Evgeny A.
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 19.12.2022
MDPI
Subjects
Aqueous solutions
Biocompatibility
Biomedical materials
Cell adhesion
Cell culture
Cell migration
Cell physiology
cells
Composition
Conductance
Cytosol
fibrillation
Gels (Pharmacy)
hydrogel
Hydrogels
Ions
Lasers
Methods
Microscope and microscopy
Microscopy
Microscopy, Fluorescence
peptide self-assembly
Peptides
pH effects
Physiological aspects
Regenerative medicine
Scanning
scanning ion conductance microscopy
Tissue engineering
Topography
Russia
United Kingdom > UK
United States > US
Berlin Germany
Germany
scanning ion conductance microscopy
cells
hydrogel
regenerative medicine
reactive oxygen species
peptide self-assembly
fibrillation
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Abstract Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and to be applied in the field of regenerative medicine. The study of the cell-matrix interaction is important for the understanding of cell attachment, proliferation, and migration, as well as for the improvement of the matrix. Here, we used scanning ion conductance microscopy (SICM) to study the growth of cells on self-assembled peptide-based hydrogels. The hydrogel surface topography, which changes during its formation in an aqueous solution, were studied at nanoscale resolution and compared with fluorescence lifetime imaging microscopy (FLIM). Moreover, SICM demonstrated the ability to map living cells inside the hydrogel. A zwitterionic label-free pH nanoprobe with a sensitivity > 0.01 units was applied for the investigation of pH mapping in the hydrogel to estimate the hydrogel applicability for cell growth. The SICM technique that was applied here to evaluate the cell growth on the peptide-based hydrogel can be used as a tool to study functional living cells.
AbstractList Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and to be applied in the field of regenerative medicine. The study of the cell-matrix interaction is important for the understanding of cell attachment, proliferation, and migration, as well as for the improvement of the matrix. Here, we used scanning ion conductance microscopy (SICM) to study the growth of cells on self-assembled peptide-based hydrogels. The hydrogel surface topography, which changes during its formation in an aqueous solution, were studied at nanoscale resolution and compared with fluorescence lifetime imaging microscopy (FLIM). Moreover, SICM demonstrated the ability to map living cells inside the hydrogel. A zwitterionic label-free pH nanoprobe with a sensitivity > 0.01 units was applied for the investigation of pH mapping in the hydrogel to estimate the hydrogel applicability for cell growth. The SICM technique that was applied here to evaluate the cell growth on the peptide-based hydrogel can be used as a tool to study functional living cells.Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and to be applied in the field of regenerative medicine. The study of the cell-matrix interaction is important for the understanding of cell attachment, proliferation, and migration, as well as for the improvement of the matrix. Here, we used scanning ion conductance microscopy (SICM) to study the growth of cells on self-assembled peptide-based hydrogels. The hydrogel surface topography, which changes during its formation in an aqueous solution, were studied at nanoscale resolution and compared with fluorescence lifetime imaging microscopy (FLIM). Moreover, SICM demonstrated the ability to map living cells inside the hydrogel. A zwitterionic label-free pH nanoprobe with a sensitivity > 0.01 units was applied for the investigation of pH mapping in the hydrogel to estimate the hydrogel applicability for cell growth. The SICM technique that was applied here to evaluate the cell growth on the peptide-based hydrogel can be used as a tool to study functional living cells.
Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and to be applied in the field of regenerative medicine. The study of the cell-matrix interaction is important for the understanding of cell attachment, proliferation, and migration, as well as for the improvement of the matrix. Here, we used scanning ion conductance microscopy (SICM) to study the growth of cells on self-assembled peptide-based hydrogels. The hydrogel surface topography, which changes during its formation in an aqueous solution, were studied at nanoscale resolution and compared with fluorescence lifetime imaging microscopy (FLIM). Moreover, SICM demonstrated the ability to map living cells inside the hydrogel. A zwitterionic label-free pH nanoprobe with a sensitivity > 0.01 units was applied for the investigation of pH mapping in the hydrogel to estimate the hydrogel applicability for cell growth. The SICM technique that was applied here to evaluate the cell growth on the peptide-based hydrogel can be used as a tool to study functional living cells.
Audience Academic
Author Erofeev, Alexander S.
Gorelkin, Petr V.
Osminkina, Liubov A.
Sysoev, Nikolay N.
Timoshenko, Roman V.
Cohen-Gerassi, Dana
Tikhonova, Tatiana N.
Kolmogorov, Vasilii S.
Vaneev, Alexander N.
Adler-Abramovich, Lihi
Shirshin, Evgeny A.
AuthorAffiliation 3 Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia
5 World-Class Research Center “Digital Biodesign and Personalized Healthcare”, Sechenov First Moscow State Medical University, 8-2 Trubetskaya St., 119991 Moscow, Russia
2 Laboratory of Biophysics, National University of Science and Technology “MISiS”, 4 Leninskiy Prospekt, 119049 Moscow, Russia
4 Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, The Center for Nanoscience and Nanotechnology, The Center for the Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv 69978, Israel
1 Department of Physics, M.V. Lomonosov Moscow State University, Leninskie Gory 1/2, 119991 Moscow, Russia
AuthorAffiliation_xml – name: 2 Laboratory of Biophysics, National University of Science and Technology “MISiS”, 4 Leninskiy Prospekt, 119049 Moscow, Russia
– name: 5 World-Class Research Center “Digital Biodesign and Personalized Healthcare”, Sechenov First Moscow State Medical University, 8-2 Trubetskaya St., 119991 Moscow, Russia
– name: 1 Department of Physics, M.V. Lomonosov Moscow State University, Leninskie Gory 1/2, 119991 Moscow, Russia
– name: 4 Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, The Center for Nanoscience and Nanotechnology, The Center for the Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv 69978, Israel
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Issue 24
Keywords scanning ion conductance microscopy
cells
hydrogel
regenerative medicine
reactive oxygen species
peptide self-assembly
fibrillation
Language English
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Snippet Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and to be applied in the field of regenerative medicine. The study of the...
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StartPage 4137
SubjectTerms Aqueous solutions
Biocompatibility
Biomedical materials
Cell adhesion
Cell culture
Cell migration
Cell physiology
cells
Composition
Conductance
Cytosol
fibrillation
Gels (Pharmacy)
hydrogel
Hydrogels
Ions
Lasers
Methods
Microscope and microscopy
Microscopy
Microscopy, Fluorescence
peptide self-assembly
Peptides
pH effects
Physiological aspects
Regenerative medicine
Scanning
scanning ion conductance microscopy
Tissue engineering
Topography
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Title Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy
URI https://www.ncbi.nlm.nih.gov/pubmed/36552900
https://www.proquest.com/docview/2756685152
https://www.proquest.com/docview/2758115856
https://pubmed.ncbi.nlm.nih.gov/PMC9776472
https://doaj.org/article/5ca99d7741174357bf1f59b4abcfe066
Volume 11
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