Graphitic Carbon Nitride Nanoheterostructures as Novel Platforms for the Electrochemical Sensing of the Chemotherapeutic and Immunomodulator Agent MTX

• Published in: Biosensors (Basel) Vol. 13; no. 1; p. 51
• Main Authors: Elfarargy, Reham G, Saleh, Mahmoud A, Abodouh, Mohamed Mahrous, Hamza, Mahmoud A, Allam, Nageh K
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.12.2022; MDPI
• Subjects: Carbon; Carbon - chemistry; Carbon nitride; Chemical bonds; Chromatography; Cost analysis; Density functional theory; DFT; Dihydrofolate reductase; Electrochemical oxidation; electrochemical sensors; Electrochemical Techniques - methods; Electrochemistry; Electrodes; Fourier transforms; Graphite; Graphite - chemistry; graphitic carbon nitride; Immunologic Factors; Immunomodulation; Immunomodulators; Inflammation; Infrared spectroscopy; LOD; Methotrexate; Nanostructure; Nitrogen; Oxidation; Rheumatoid arthritis; Scanning electron microscopy; X-ray diffraction; United States > US; LOD; methotrexate; DFT; electrochemical sensors; graphitic carbon nitride
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13010051

We report on the electrochemical determination of one the most effective and widely used chemotherapeutic, anti-inflammatory, and immunomodulator agents, methotrexate (MTX), using low-cost, green, and facile one-pot prepared graphitic carbon nitride (g-CN ) nanosheets. The g-CN nanosheets have been characterized utilizing Fourier transform infrared spectroscopy, X-ray diffraction(XRD), scanning electron microscopy(SEM), and density functional theory (DFT). In comparison to the bare carbon paste electrode (CPE), the g-CN -modified electrode showed a spectacular enhancement in the electrochemical oxidation and detection abilities of MTX. The proposed material exhibits very low limits of detection (12.45 nM) and quantification (41.5 nM), while possessing a wide linear range of 0.22-1.11 μM and 1.11-27.03 μM under optimized conditions at pH 7.0. Due to the ease of preparation of g-CN, it can be adopted for the cost-effective detection of MTX in industrial and clinical analyses.