Antioxidant Capacity and Radical Scavenging Effect of Polyphenol Rich Mallotus philippenensis Fruit Extract on Human Erythrocytes: An In Vitro Study

• Published in: TheScientificWorld Vol. 2014; no. 2014; pp. 1 - 12
• Main Authors: Goel, R. K., Tripathi, Yamini B., Sharma, Amit Kumar, Gautam, M. K., Gangwar, Mayank, Nath, Gopal
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 2014; John Wiley & Sons, Inc; Wiley
• Subjects: Acetophenones - pharmacology; Antioxidants; Antioxidants - pharmacology; Benzopyrans - pharmacology; Benzothiazoles - metabolism; Biphenyl Compounds - metabolism; Carbohydrates; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Chronic illnesses; Erythrocytes; Erythrocytes - drug effects; Ethanol; Euphorbiaceae; Flavonoids; Free Radical Scavengers - pharmacology; Free radicals; Free radicals (Chemistry); Fruit - chemistry; Fruits; Health aspects; Hemolysis - drug effects; Humans; Hydroxyl Radical; Hydroxyl radicals; Laboratories; Lipid Peroxidation - drug effects; Lipids; Liquid chromatography; Mallotus Plant - chemistry; Materia medica, Vegetable; Medicinal plants; Metabolic disorders; Nitric oxide; Nitric Oxide - metabolism; Oxidation-Reduction - drug effects; Peroxidation; Pharmaceutical sciences; Phenols; Phytochemicals; Phytochemicals - pharmacology; Picrates - metabolism; Plant extracts; Plant Extracts - pharmacology; Polyphenols; Polyphenols - pharmacology; Potassium; Reference Standards; Scholarships & fellowships; Sulfonic Acids - metabolism
• DOI: 10.1155/2014/279451
• ISSN: 2356-6140; 1537-744X

Mallotus philippinensis is an important source of molecules with strong antioxidant activity widely used medicinal plant. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. So, present investigation was designed to evaluate the total antioxidant activity and radical scavenging effect of 50% ethanol fruit glandular hair extract (MPE) and its role on Human Erythrocytes. MPE was tested for phytochemical test followed by its HPLC analysis. Standard antioxidant assays like DPPH, ABTS, hydroxyl, superoxide radical, nitric oxide, and lipid peroxidation assay were determined along with total phenolic and flavonoids content. Results showed that MPE contains the presence of various phytochemicals, with high total phenolic and flavonoid content. HPLC analysis showed the presence of rottlerin, a polyphenolic compound in a very rich quantity. MPE exhibits significant strong scavenging activity on DPPH and ABTS assay. Reducing power showed dose dependent increase in concentration absorption compared to standard, Quercetin. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. Our finding further provides evidence that Mallotus fruit extract is a potential natural source of antioxidants which have a protective role on human Erythrocytes exhibiting minimum hemolytic activity and this justified its uses in folklore medicines.