Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus

• Published in: Life (Basel, Switzerland) Vol. 12; no. 1; p. 72
• Main Authors: Nedeljković, Gordana, Mazija, Hrvoje, Cvetić, Željko, Jergović, Mladen, Bendelja, Krešo, Gottstein, Željko
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.01.2022; MDPI
• Subjects: Animals; Antibodies; Antibody response; Anticoagulants; Cell proliferation; Cell-mediated immunity; chicken; Chickens; Disease; Disease control; Epidemics; Feeds; Flow cytometry; Helper cells; Hemagglutination inhibition; Immune response; Immunization; Immunogenicity; Infections; Influenza; Laboratories; Leukocytes; Lymphocytes; Lymphocytes T; Mortality; Newcastle disease; Poultry; R&D; Research & development; Strains (organisms); Subpopulations; Vaccines; Virulence; Viruses; Weaning; ZG1999HDS strain; Croatia; Newcastle disease; chicken; antibody response; ZG1999HDS strain; immunization; cell-mediated immunity
• DOI: 10.3390/life12010072
• ISSN: 2075-1729

Newcastle disease (ND) is a highly contagious avian disease. Global control of ND is mainly based on vaccination of poultry; however, reported outbreaks of ND in vaccinated flocks indicate a constant need to re-evaluate the existing vaccines and a development of the new ones. In this study, 4-week-old male chickens of the layer commercial hybrid were immunized oculonasally with a commercial NDV live La Sota vaccine (LS group), a suspension of lyophilized NDV strain ZG1999HDS (ZG group), or saline (Control (K) group). Antibody response was determined by haemagglutination inhibition (HI) assay. Cell-mediated immunity (CMI) was characterized by immunophenotyping of leukocyte's and T-lymphocyte's subpopulations (flow cytometry). Applied NDV strains did not cause any adverse reaction in treated chickens. Both strains induced the significantly higher HI antibody response in comparison to the control group, and overall antibody titer was higher in ZG group than in LS group. CMI, manifested as a higher proliferation of B- and T-helper cells, yielded better results in the ZG groups than in the LS group. Based on the obtained results, we conclude that the strain ZG1999HDS is immunogenic and is a suitable candidate for further research and development of poultry vaccines.