Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis

Women are at a significantly higher risk of developing osteoarthritis (OA) compared to males. The pathogenesis of osteoarthritis (OA) in women is poorly understood. Extracellular vesicles (EVs) have been shown to play an essential role in numerous signaling processes during the pathogenesis of age-r...

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Published inLife (Basel, Switzerland) Vol. 10; no. 12; p. 337
Main Authors Kolhe, Ravindra, Owens, Virgenal, Sharma, Ashok, Lee, Tae Jin, Zhi, Wenbo, Ghilzai, Umar, Mondal, Ashis K, Liu, Yutao, Isales, Carlos M, Hamrick, Mark W, Hunter, Monte, Fulzele, Sadanand
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 10.12.2020
MDPI
Subjects
Acetyltransferase
Acids
Age
Age related diseases
Annotations
Apolipoproteins
Arthritis
Biomarkers
Biomedical materials
Brief Report
Cargo
cartilage degeneration
Ceruloplasmin
Diabetes
Disease
exosomes
Gender
Glycoproteins
Haptoglobin
Inflammation
Males
Mass spectrometry
miRNA
Osteoarthritis
Paracrine signalling
Pathogenesis
Patients
Peptides
Protein expression
Proteins
Scientific imaging
Sex
Signaling
Statistical analysis
Synovial fluid
Womens health
United States > US
mass spectrometry
cartilage degeneration
gender
exosomes
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Abstract Women are at a significantly higher risk of developing osteoarthritis (OA) compared to males. The pathogenesis of osteoarthritis (OA) in women is poorly understood. Extracellular vesicles (EVs) have been shown to play an essential role in numerous signaling processes during the pathogenesis of age-related diseases via paracrine signaling. Molecular profiling of the synovial fluid-derived EVs cargo in women may help in the discovery of novel biomarkers and therapeutics for the treatment of OA in women. Previously, we reported that synovial fluid-derived EV miRNA cargo differs in a sex-specific manner. This study aims to characterize synovial fluid-derived EV protein cargo in OA patients. Our data showed sex-specific EVs protein content in OA. We found haptoglobin, orosomucoid, and ceruloplasmin significantly up-regulated, whereas apolipoprotein down-regulated in female OA EVs. In males, we discovered β-2-glycoprotein, and complement component 5 proteins significantly up-regulated and Spt-Ada-Gcn5 acetyltransferase (SAGA)-associated factor 29 down-regulated in male OA EVs. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analysis revealed OA-specific protein involvement in several biological, molecular, and cellular pathways, specifically in inflammatory processes. In conclusion, synovial fluid EV protein content is altered in a sex-specific manner with OA, explaining the increased prevalence and severity of OA in women.
AbstractList Women are at a significantly higher risk of developing osteoarthritis (OA) compared to males. The pathogenesis of osteoarthritis (OA) in women is poorly understood. Extracellular vesicles (EVs) have been shown to play an essential role in numerous signaling processes during the pathogenesis of age-related diseases via paracrine signaling. Molecular profiling of the synovial fluid-derived EVs cargo in women may help in the discovery of novel biomarkers and therapeutics for the treatment of OA in women. Previously, we reported that synovial fluid-derived EV miRNA cargo differs in a sex-specific manner. This study aims to characterize synovial fluid-derived EV protein cargo in OA patients. Our data showed sex-specific EVs protein content in OA. We found haptoglobin, orosomucoid, and ceruloplasmin significantly up-regulated, whereas apolipoprotein down-regulated in female OA EVs. In males, we discovered β-2-glycoprotein, and complement component 5 proteins significantly up-regulated and Spt-Ada-Gcn5 acetyltransferase (SAGA)-associated factor 29 down-regulated in male OA EVs. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analysis revealed OA-specific protein involvement in several biological, molecular, and cellular pathways, specifically in inflammatory processes. In conclusion, synovial fluid EV protein content is altered in a sex-specific manner with OA, explaining the increased prevalence and severity of OA in women.
Author Kolhe, Ravindra
Mondal, Ashis K
Fulzele, Sadanand
Isales, Carlos M
Ghilzai, Umar
Hunter, Monte
Hamrick, Mark W
Owens, Virgenal
Liu, Yutao
Sharma, Ashok
Lee, Tae Jin
Zhi, Wenbo
AuthorAffiliation 7 Institute of Healthy Aging, Augusta University, Augusta, GA 30912, USA
3 Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28203, USA
5 Cell Biology and Anatomy, Augusta University, Augusta, GA 30912, USA; YUTLIU@augusta.edu
4 Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA 30912, USA; ASSHARMA@augusta.edu (A.S.); TALEE@augusta.edu (T.J.L.); WZHI@augusta.edu (W.Z.)
1 Department of Pathology, Augusta University, Augusta, GA 30912, USA; rkolhe@augusta.edu (R.K.); AMONDAL@augusta.edu (A.K.M.); cisales@augusta.edu (C.M.I.); mhamrick@augusta.edu (M.W.H.)
6 Department of Medicine, Augusta University, Augusta, GA 30912, USA
2 Department of Orthopaedic Surgery, Augusta University, Augusta, GA 30912, USA; virgenal.owens@atriumhealth.org (V.O.); UGHILZAI@augusta.edu (U.G.); MOHUNTER@augusta.edu (M.H.)
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– name: 6 Department of Medicine, Augusta University, Augusta, GA 30912, USA
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  surname: Fulzele
  fullname: Fulzele, Sadanand
  organization: Institute of Healthy Aging, Augusta University, Augusta, GA 30912, USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33321751$$D View this record in MEDLINE/PubMed
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cartilage degeneration
gender
exosomes
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Snippet Women are at a significantly higher risk of developing osteoarthritis (OA) compared to males. The pathogenesis of osteoarthritis (OA) in women is poorly...
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SubjectTerms Acetyltransferase
Acids
Age
Age related diseases
Annotations
Apolipoproteins
Arthritis
Biomarkers
Biomedical materials
Brief Report
Cargo
cartilage degeneration
Ceruloplasmin
Diabetes
Disease
exosomes
Gender
Glycoproteins
Haptoglobin
Inflammation
Males
Mass spectrometry
miRNA
Osteoarthritis
Paracrine signalling
Pathogenesis
Patients
Peptides
Protein expression
Proteins
Scientific imaging
Sex
Signaling
Statistical analysis
Synovial fluid
Womens health
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Title Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis
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Volume 10
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