Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylate synthase from melon fruits

• Published in: Plant physiology (Bethesda) Vol. 107; no. 1; pp. 297 - 298
• Main Authors: Miki, T. (Chiba University, Chiba (Japan)), Yamamoto, M, Nakagawa, H, Ogura, N, Mori, H, Imaseki, H, Sato, T
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.01.1995
• Subjects: ACC; ADN; AMINO ACID SEQUENCES; Amino acids; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; CHEMICAL COMPOSITION; CLONE; CLONES; CODE GENETIQUE; CODIGO GENETICO; Complementary DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CUCUMIS MELO; DNA; DNA, Complementary; ENREGISTREMENT; Enzymes and enzyme inhibitors; Ethylene production; Fruit - enzymology; Fruit - genetics; FRUITS; FRUTAS; Fundamental and applied biological sciences. Psychology; genbank/d30805; GENE; GENES; GENETIC CODE; Horticulture; LIGASAS; LIGASE; LIGASES; Lyases; Lyases - genetics; Mesocarp; Messenger RNA; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Plant Gene Register; PLANT GROWTH SUBSTANCES; Plants; REGISTRATION; REGISTRO; Ripening; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; Theology; 1-Aminocyclopropane-1-carboxylate synthase; Fruit; Nucleotide sequence; Enzyme; Injury; Cucurbitaceae; Mesocarp; Homology; Lyases; Gene expression; Ripening; Complementary DNA; Fruit crop; Dicotyledones; Angiospermae; Carbon-sulfur lyases; Spermatophyta; Cucumis melo; Comparative study; Aminoacid sequence
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.107.1.297

Ethylene is a plant hormone that has an essential role in fruit ripening. ACC synthase (S-adenosyl-L-methionine methylethioadenosine-lyase, EC 4.4.1.14), which is encoded by a multigene family, plays a regulatory role in ethylene production. Several genes for ACC synthase have been isolated from tomato, mung bean, winter squash and Arabidopsis. We isolated a cDNA (pMEACS1, 2097 bp) for ACC synthase from wounded mesocarp tissue from melon fruits (Cucumis melo L. cv AMS). Sequence analysis of this cDNA revealed the presence of an open reading frame of 493 amino acids. This polypeptide contained seven sequences that were conserved among other ACC synthases. RNA blot analysis showed that the level of mRNA for the gene increased in the mesocarp tissue of melon fruits after wounding and also during ripening.