Activation of CYP1A1 gene expression during primary culture of mouse hepatocytes

• Published in: Toxicology (Amsterdam) Vol. 216; no. 2; pp. 224 - 231
• Main Authors: Tamaki, Hisako, Sakuma, Tsutomu, Uchida, Yo-ichi, Jaruchotikamol, Atika, Nemoto, Nobuo
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.12.2005; Amsterdam Elsevier Science
• Subjects: Animals; Anti-oxidant; Biological and medical sciences; Cells, Cultured; CYP1A1; CYP1A2; Cytochrome P-450 CYP1A1 - drug effects; Cytochrome P-450 CYP1A1 - genetics; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P-450 CYP1A2 - genetics; Electrophoretic Mobility Shift Assay - methods; Enzyme Inhibitors - pharmacology; Gene Expression Regulation, Enzymologic - drug effects; Genistein - pharmacology; Hepatocytes - enzymology; Hydrogen Peroxide - pharmacology; Hydroquinones - pharmacology; Insulin - pharmacology; Isoflavones - pharmacology; Kinetin - pharmacology; Medical sciences; Mice; Mice, Inbred C57BL; Mouse primary culture; Okadaic acid; Okadaic Acid - pharmacology; Oxidative stress; Phosphorylation - drug effects; Protein kinase inhibitor; Purines - pharmacology; RNA, Messenger - drug effects; Time Factors; Toxicology; Transcriptional Activation; Oxidative stress; SSC; Hsp; Anti-oxidant; Arnt; TCDD; AhR; XRE; Protein kinase inhibitor; DCFH-DA; SDS-PAGE; CYP1A2; NF-1; CYP1A1; Okadaic acid; cDNA; CYP; GAPDH; Mouse primary culture; Protein inhibitor; Digestive system; Enzyme; Liver; Transferases; Cytochrome P450; Rodentia; Antioxidant; Gene expression; In vitro; Vertebrata; Mammalia; Hepatocyte; Mouse; Protein kinase; Animal; Primary culture

Expression of CYP1A1 mRNA in mouse hepatocytes in primary culture was investigated. The expression was obvious on day 3 of culture without addition of any known ligands of the aryl hydrocarbon receptor and increased with culture period. Removal of insulin from and addition of hydrogen peroxide to th...