Loop Swapping in an Antisense RNA/Target RNA Pair Changes Directionality of Helix Progression

• Published in: The Journal of biological chemistry Vol. 278; no. 37; pp. 35558 - 35563
• Main Authors: Slagter-Jäger, Jacoba G., Wagner, E.Gerhart H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.09.2003; American Society for Biochemistry and Molecular Biology
• Subjects: Bacterial Proteins - genetics; Base Pairing; Base Sequence; Binding Sites; Coat Protein Complex I - genetics; DNA Primers; Kinetics; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Antisense - chemistry; RNA, Antisense - genetics

The binding pathway of the natural antisense RNA CopA to its target CopT proceeds through a hierarchical order of steps. It initiates by reversible loop-loop contacts followed by unidirectional helix progression into the upper stems. This involves extensive breakage of intramolecular base pairs and...