Molecular and Biochemical Characterization of Three Anthocyanin Synthetic Enzymes from Gentiana triflora

• Published in: Plant and cell physiology Vol. 37; no. 5; pp. 711 - 716
• Main Authors: Tanaka, Yoshikazu, Yonekura, Keiko, Fukuchi-Mizutani, Masako, Fukui, Yuko, Fujiwara, Hiroyuki, Ashikari, Toshihiko, Kusumi, Takaaki
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.07.1996
• Subjects: Alcohol Oxidoreductases - chemistry; Alcohol Oxidoreductases - genetics; Alcohol Oxidoreductases - metabolism; Amino Acid Sequence; Anthocyanin; Anthocyanins - biosynthesis; Base Sequence; Biological and medical sciences; Cloning; Cytochrome P-450 Enzyme System - chemistry; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; DNA, Complementary; DNA, Plant; Flavonoid; Fundamental and applied biological sciences. Psychology; Gene Expression; Gentian {Gentiana triflora; Glucosyltransferases - chemistry; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Metabolism; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; Molecular Sequence Data; Plant physiology and development; Plant Proteins - genetics; Plants - enzymology; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Storage and secretion, pigments, phytochrome; Anthocyanin; Flower; Enzyme; Homology; Biosynthesis; Metabolism; Dihydrokaempferol 4-reductase; Gene expression; Complementary DNA; Dicotyledones; Angiospermae; Gentianaceae; Pigments; Isolation; Spermatophyta; Oxidoreductases; Aminoacid sequence
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029004

Full length cDNA clones of flavonoid 3′,5′-hydroxylase, dihydroflavonol 4-reductase and flavonoid 3-glucosyltransferase were cloned from petals of Gentiana triflora. Their sequences were homologous to counterparts from other plants. Flavonoid 3′,5′-hydroxylase and flavonoid 3-glucosyltransferase were enzymatically characterized by expressing cDNAs in heterologous expression systems.