Quantification of Leptospira interrogans Survival in Soil and Water Microcosms

• Published in: Applied and environmental microbiology Vol. 84; no. 13; p. e00507-18
• Main Authors: Casanovas-Massana, Arnau, Pedra, Gabriel Ghizzi, Wunder, Elsio A., Diggle, Peter J., Begon, Mike, Ko, Albert I.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.07.2018
• Subjects: Animals; Decay; Decay rate; Deoxyribonucleic acid; Disease transmission; DNA; DNA, Bacterial - analysis; Environmental Microbiology; Etiology; Genetic Markers; Gram-negative bacteria; Humans; Leptospira; Leptospira interrogans; Leptospira interrogans - genetics; Leptospira interrogans - growth & development; Leptospira interrogans - isolation & purification; Leptospira interrogans - pathogenicity; Leptospirosis; Leptospirosis - microbiology; Leptospirosis - transmission; Low concentrations; Markers; Microbial Viability; Microcosms; Polymerase chain reaction; Real-Time Polymerase Chain Reaction; Sewage; Sewage - microbiology; Soil; Soil contamination; Soil Microbiology; Soil pollution; Soil water; Soils; Spring water; Survival; Survival analysis; Time Factors; Urine; Water; Water Microbiology; Water pollution; Waterborne Diseases - microbiology; Waterborne Diseases - transmission; Zoonoses; waterborne pathogens; survival; persistence; statistical modeling; qPCR; soil; soil microbiology; water; sewage; Leptospira

Leptospira interrogans is the etiological agent of leptospirosis, a globally distributed zoonotic disease. Human infection usually occurs through skin exposure with water and soil contaminated with the urine of chronically infected animals. In this study, we aimed to quantitatively characterize the...