Selection of Biophysical Methods for Characterisation of Membrane Proteins

• Published in: International journal of molecular sciences Vol. 20; no. 10; p. 2605
• Main Authors: Kwan, Tristan O C, Reis, Rosana, Siligardi, Giuliano, Hussain, Rohanah, Cheruvara, Harish, Moraes, Isabel
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.05.2019; MDPI
• Subjects: Automation; biophysics; Brownian motion; Cell adhesion & migration; Chromatography; circular dichroism; Crystallography; Diffusion coefficient; Diffusion rate; Drug development; Drugs; dynamic light scattering; Einstein equations; G protein-coupled receptors; Ion channels; Laboratories; LCP-FRAP; Ligands; Light; Lipids; Membrane proteins; Membranes; Methods; MIR; NMR; Nuclear magnetic resonance; Protein structure; Proteins; Quality assessment; Researchers; Review; SEC-MALS; Spectrum analysis; Structure-function relationships; Temperature; Translation; Viscosity; dynamic light scattering; membrane proteins; SEC-MALS; LCP-FRAP; MIR; circular dichroism; biophysics

Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure-function relationships. However, the study of integral membrane p...