Natural occurrence of ochratoxin A in dried figs
Ochratoxin A (OTA) contamination in dried figs was investigated using high performance liquid chromatography (HPLC) with fluorescence detection after extraction with methanol and orthophosphoric acid and clean up by an immunoaffinity column. The limit of detection for OTA was 0.12 μg kg −1. One hund...
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| Published in | Analytica chimica acta Vol. 617; no. 1; pp. 32 - 36 |
|---|---|
| Main Authors | , |
| Format | Journal Article Conference Proceeding |
| Language | English |
| Published |
Amsterdam
Elsevier B.V
09.06.2008
Elsevier |
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| Abstract | Ochratoxin A (OTA) contamination in dried figs was investigated using high performance liquid chromatography (HPLC) with fluorescence detection after extraction with methanol and orthophosphoric acid and clean up by an immunoaffinity column. The limit of detection for OTA was 0.12
μg
kg
−1. One hundred and fifteen samples were taken during the drying stage from 7 different districts in the Aegean Region in 2003 and 2004. Fifty-five (47.2%) of the 115 samples were found to contain detectable levels of ochratoxin A, ranging from 0.12 to 15.31
μg
kg
−1. However, the OTA level for a majority of the samples was low, with only 4 samples containing OTA exceeding 1
μg
kg
−1. The calculated overall median for the OTA level was below the limit of detection and the overall mean was estimated as 0.52
μg
kg
−1. Frequency of ochratoxin A contamination in dried figs harvested in 2003 and 2004 are 47 and 50%, respectively. Highest contamination ratio was determined in dried figs from Erbeyli (60%), followed by Selcuk (56%), and Ortaklar (50%). |
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| AbstractList | Ochratoxin A (OTA) contamination in dried figs was investigated using high performance liquid chromatography (HPLC) with fluorescence detection after extraction with methanol and orthophosphoric acid and clean up by an immunoaffinity column. The limit of detection for OTA was 0.12 mug kg-1. One hundred and fifteen samples were taken during the drying stage from 7 different districts in the Aegean Region in 2003 and 2004. Fifty-five (47.2%) of the 115 samples were found to contain detectable levels of ochratoxin A, ranging from 0.12 to 15.31 mug kg-1. However, the OTA level for a majority of the samples was low, with only 4 samples containing OTA exceeding 1 mug kg-1. The calculated overall median for the OTA level was below the limit of detection and the overall mean was estimated as 0.52 mug kg-1. Frequency of ochratoxin A contamination in dried figs harvested in 2003 and 2004 are 47 and 50%, respectively. Highest contamination ratio was determined in dried figs from Erbeyli (60%), followed by Selcuk (56%), and Ortaklar (50%). Ochratoxin A (OTA) contamination in dried figs was investigated using high performance liquid chromatography (HPLC) with fluorescence detection after extraction with methanol and orthophosphoric acid and clean up by an immunoaffinity column. The limit of detection for OTA was 0.12 μg kg −1. One hundred and fifteen samples were taken during the drying stage from 7 different districts in the Aegean Region in 2003 and 2004. Fifty-five (47.2%) of the 115 samples were found to contain detectable levels of ochratoxin A, ranging from 0.12 to 15.31 μg kg −1. However, the OTA level for a majority of the samples was low, with only 4 samples containing OTA exceeding 1 μg kg −1. The calculated overall median for the OTA level was below the limit of detection and the overall mean was estimated as 0.52 μg kg −1. Frequency of ochratoxin A contamination in dried figs harvested in 2003 and 2004 are 47 and 50%, respectively. Highest contamination ratio was determined in dried figs from Erbeyli (60%), followed by Selcuk (56%), and Ortaklar (50%). Ochratoxin A (OTA) contamination in dried figs was investigated using high performance liquid chromatography (HPLC) with fluorescence detection after extraction with methanol and orthophosphoric acid and clean up by an immunoaffinity column. The limit of detection for OTA was 0.12 microg kg(-1). One hundred and fifteen samples were taken during the drying stage from 7 different districts in the Aegean Region in 2003 and 2004. Fifty-five (47.2%) of the 115 samples were found to contain detectable levels of ochratoxin A, ranging from 0.12 to 15.31 microg kg(-1). However, the OTA level for a majority of the samples was low, with only 4 samples containing OTA exceeding 1 microg kg(-1). The calculated overall median for the OTA level was below the limit of detection and the overall mean was estimated as 0.52 microg kg(-1). Frequency of ochratoxin A contamination in dried figs harvested in 2003 and 2004 are 47 and 50%, respectively. Highest contamination ratio was determined in dried figs from Erbeyli (60%), followed by Selcuk (56%), and Ortaklar (50%). |
| Author | Heperkan, Dilek Karbancıoğlu-Güler, Funda |
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| Keywords | Ochratoxin A Dried figs High performance liquid chromatography Mycotoxin Ochratoxin Fluorescence detector Fig HPLC chromatography Contamination Toxin Sample preparation Detection limit Dried fruit Methanol Drying |
| Language | English |
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| SubjectTerms | Analytical chemistry Biological Products - analysis Biological Products - biosynthesis Chemistry Chromatographic methods and physical methods associated with chromatography Desiccation Dried figs Exact sciences and technology Ficus - chemistry Ficus - metabolism High performance liquid chromatography Mycotoxin Ochratoxin A Ochratoxins - analysis Ochratoxins - biosynthesis Other chromatographic methods Time Factors Water - chemistry |
| Title | Natural occurrence of ochratoxin A in dried figs |
| URI | https://dx.doi.org/10.1016/j.aca.2008.01.009 https://www.ncbi.nlm.nih.gov/pubmed/18486638 https://search.proquest.com/docview/33400344 |
| Volume | 617 |
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