A Real-Time PCR Method for the Authentication of Common Cuttlefish ( Sepia officinalis ) in Food Products

• Published in: Foods Vol. 9; no. 3; p. 286
• Main Authors: Velasco, Amaya, Ramilo-Fernández, Graciela, Sotelo, Carmen G
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.03.2020; MDPI
• Subjects: Amplification; Cephalopods; coi (cytochrome oxidase i); common cuttlefish; Consumers; Cross-reactivity; Cytochrome oxidase I; Cytochromes; Deoxyribonucleic acid; Design; DNA; Food; food authentication; Food resources; Food science; Fraud; Grooves; Identification; Methods; Mitochondria; Phylogenetics; Polymerase chain reaction; Real time; real-time pcr (polymerase chain reaction); Sepia; Sepia officinalis; Species; species identification; Value chain; United States > US; Chicago Illinois; Spain; common cuttlefish; food authentication; real-time PCR (Polymerase Chain Reaction); Sepia officinalis; species identification; Sepia; COI (Cytochrome Oxidase I)

Cephalopods are very relevant food resources. The common cuttlefish ( ) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authe...