iTRAQ-Based Quantitative Proteomic Analysis of Embryogenic and Non-embryogenic Calli Derived from a Maize ( Zea mays L.) Inbred Line Y423

Somatic embryos (SE) have potential to rapidly form a whole plant. Generally, SE is thought to be derived from embryogenic calli (EC). However, in maize, not only embryogenic calli (EC, can generate SE) but also nonembryogenic calli (NEC, can't generate SE) can be induced from immature embryos....

Full description

Saved in:
Bibliographic Details
Published inInternational journal of molecular sciences Vol. 19; no. 12; p. 4004
Main Authors Liu, Beibei, Shan, Xiaohui, Wu, Ying, Su, Shengzhong, Li, Shipeng, Liu, Hongkui, Han, Junyou, Yuan, Yaping
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 12.12.2018
MDPI
Subjects
Agricultural biotechnology
Bioinformatics
Biosynthesis
Callus
Cell cycle
Cell division
Embryos
Gluconeogenesis
Glycolysis
Inbreeding
iTRAQ
Metabolism
Peptides
Proteins
proteomics
pyruvate biosynthesis
Pyruvic acid
Signal transduction
somatic embryogenesis
Somatic embryos
Sucrose
Tricarboxylic acid cycle
Zea mays
iTRAQ
Zea mays
proteomics
pyruvate biosynthesis
somatic embryogenesis
Online AccessGet full text

Cover

Abstract Somatic embryos (SE) have potential to rapidly form a whole plant. Generally, SE is thought to be derived from embryogenic calli (EC). However, in maize, not only embryogenic calli (EC, can generate SE) but also nonembryogenic calli (NEC, can't generate SE) can be induced from immature embryos. In order to understand the differences between EC and NEC and the mechanism of EC, which can easily form SE in maize, differential abundance protein species (DAPS) of EC and NEC from the maize inbred line Y423 were identified by using the isobaric tags for relative and absolute quantification (iTRAQ) proteomic technology. We identified 632 DAPS in EC compared with NEC. The results of bioinformatics analysis showed that EC development might be related to accumulation of pyruvate caused by the DAPS detected in some pathways, such as starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle, fatty acid metabolism and phenylpropanoid biosynthesis. Based on the differentially accumulated proteins in EC and NEC, a series of DAPS related with pyruvate biosynthesis and suppression of acetyl-CoA might be responsible for the differences between EC and NEC cells. Furthermore, we speculate that the decreased abundance of enzymes/proteins involved in phenylpropanoid biosynthesis pathway in the EC cells results in reducing of lignin substances, which might affect the maize callus morphology.
AbstractList Somatic embryos (SE) have potential to rapidly form a whole plant. Generally, SE is thought to be derived from embryogenic calli (EC). However, in maize, not only embryogenic calli (EC, can generate SE) but also nonembryogenic calli (NEC, can't generate SE) can be induced from immature embryos. In order to understand the differences between EC and NEC and the mechanism of EC, which can easily form SE in maize, differential abundance protein species (DAPS) of EC and NEC from the maize inbred line Y423 were identified by using the isobaric tags for relative and absolute quantification (iTRAQ) proteomic technology. We identified 632 DAPS in EC compared with NEC. The results of bioinformatics analysis showed that EC development might be related to accumulation of pyruvate caused by the DAPS detected in some pathways, such as starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle, fatty acid metabolism and phenylpropanoid biosynthesis. Based on the differentially accumulated proteins in EC and NEC, a series of DAPS related with pyruvate biosynthesis and suppression of acetyl-CoA might be responsible for the differences between EC and NEC cells. Furthermore, we speculate that the decreased abundance of enzymes/proteins involved in phenylpropanoid biosynthesis pathway in the EC cells results in reducing of lignin substances, which might affect the maize callus morphology.
Author Han, Junyou
Su, Shengzhong
Shan, Xiaohui
Li, Shipeng
Liu, Beibei
Wu, Ying
Yuan, Yaping
Liu, Hongkui
AuthorAffiliation College of Plant Science, Jilin University, Changchun 130062, China; liubeibei1985@126.com (B.L.); shanxiaohui@jlu.edu.cn (X.S.); wuying@jlu.edu.cn (Y.W.); sushengzhong@jlu.edu.cn (S.S.); lisp@jlu.edu.cn (S.L.); liuhk@jlu.edu.cn (H.L.); hanjy@jlu.edu.cn (J.H.)
AuthorAffiliation_xml – name: College of Plant Science, Jilin University, Changchun 130062, China; liubeibei1985@126.com (B.L.); shanxiaohui@jlu.edu.cn (X.S.); wuying@jlu.edu.cn (Y.W.); sushengzhong@jlu.edu.cn (S.S.); lisp@jlu.edu.cn (S.L.); liuhk@jlu.edu.cn (H.L.); hanjy@jlu.edu.cn (J.H.)
Author_xml – sequence: 1
  givenname: Beibei
  surname: Liu
  fullname: Liu, Beibei
  email: liubeibei1985@126.com
  organization: College of Plant Science, Jilin University, Changchun 130062, China. liubeibei1985@126.com
– sequence: 2
  givenname: Xiaohui
  surname: Shan
  fullname: Shan, Xiaohui
  email: shanxiaohui@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. shanxiaohui@jlu.edu.cn
– sequence: 3
  givenname: Ying
  surname: Wu
  fullname: Wu, Ying
  email: wuying@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. wuying@jlu.edu.cn
– sequence: 4
  givenname: Shengzhong
  surname: Su
  fullname: Su, Shengzhong
  email: sushengzhong@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. sushengzhong@jlu.edu.cn
– sequence: 5
  givenname: Shipeng
  surname: Li
  fullname: Li, Shipeng
  email: lisp@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. lisp@jlu.edu.cn
– sequence: 6
  givenname: Hongkui
  surname: Liu
  fullname: Liu, Hongkui
  email: liuhk@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. liuhk@jlu.edu.cn
– sequence: 7
  givenname: Junyou
  surname: Han
  fullname: Han, Junyou
  email: hanjy@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. hanjy@jlu.edu.cn
– sequence: 8
  givenname: Yaping
  surname: Yuan
  fullname: Yuan, Yaping
  email: yuanyp@jlu.edu.cn
  organization: College of Plant Science, Jilin University, Changchun 130062, China. yuanyp@jlu.edu.cn
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30545080$$D View this record in MEDLINE/PubMed
BookMark eNpdkktvEzEURkeoiD5gxxpZYlMkpvg5Y2-QQigQKTyKygI21vVjgqMZu9iTSuEf8K8ZSKlSVr767tGRLX_H1UFM0VfVY4LPGFP4RVgPhShCOcb8XnVEOKU1xk17sDcfVselrDGmjAr1oDpkWHCBJT6qfoXLz7OL-hUU79DFBuIYRhjDtUefchp9GoJFswj9toSCUofOB5O3aeXjlEN06EOKtd_L5tD3Ab32eVI41OU0IEDvIfz06BR984AG2Ba0PHuGFtHkCVmG6NFXTtnD6n4HffGPbs6T6sub88v5u3r58e1iPlvWlrdyrFvcUmUEN1JRipl3zinWcWUFAWOMk0Yy33JlsLKmaVnXOSE9tZ1kjfFg2Em12HldgrW-ymGAvNUJgv4bpLzSkMdge68pc8RhEBaajmOOjbMtoQDYiE7ZRkyulzvX1cYM3lkfxwz9HendTQzf9Spd64ZRQiSfBKc3gpx-bHwZ9RCK9X0P0adN0ZSItmmlUHJCn_6HrtMmT18zUUJSyTlhbKKe7yibUynZd7eXIVj_6Yve78uEP9l_wC38ryDsN4uOvVM
CitedBy_id crossref_primary_10_1016_j_scienta_2021_110680
crossref_primary_10_3390_ijms20235956
crossref_primary_10_1186_s12864_019_6325_6
crossref_primary_10_3390_ijms20102495
crossref_primary_10_1016_j_bbapap_2020_140561
crossref_primary_10_1016_j_plaphy_2023_02_045
crossref_primary_10_3390_ijms231911556
crossref_primary_10_3390_ijms222111807
crossref_primary_10_3389_fpls_2020_589005
crossref_primary_10_3390_agriculture12030393
crossref_primary_10_1007_s11240_022_02284_7
crossref_primary_10_3390_cimb45030130
crossref_primary_10_3390_cimb45070332
crossref_primary_10_1016_j_jprot_2022_104790
crossref_primary_10_35193_bseufbd_667838
Cites_doi 10.1111/j.1399-3054.2008.01129.x
10.1002/pmic.200300803
10.1007/s11103-017-0681-x
10.1111/j.1440-1835.2004.tb00334.x
10.1080/07352689109382306
10.1038/nrm3772
10.1023/A:1015243600325
10.1016/j.stem.2010.07.011
10.1016/S0168-9452(00)00382-4
10.1038/emboj.2011.401
10.1007/s00299-008-0633-7
10.1016/j.jprot.2014.03.013
10.1007/s00425-006-0238-8
10.1007/BF00251161
10.1023/A:1006033622928
10.3389/fphar.2017.00958
10.1007/BF02942513
10.1007/BF00233157
10.1007/s00299-007-0438-0
10.1007/978-1-61779-818-4_14
10.1016/j.plaphy.2015.04.011
10.1007/BF00194009
10.1111/j.1399-3054.2012.01633.x
10.1126/science.327.5967.797
10.1016/j.stem.2012.11.022
10.1038/nbt0386-219
10.1007/s00299-005-0013-5
10.3389/fpls.2015.01063
10.1007/s002990050588
10.1007/s11240-012-0255-1
10.1093/jxb/erj168
10.1038/nbt0696-745
10.1111/j.1744-7909.2009.00824.x
10.1111/j.1469-8137.2009.03113.x
10.1016/j.jprot.2014.02.003
10.1007/s11240-011-0093-6
10.1007/s00425-011-1554-1
10.1371/journal.pone.0119987
10.1038/nbt.2707
10.1007/BF00224181
ContentType Journal Article
Copyright 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2018 by the authors. 2018
Copyright_xml – notice: 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2018 by the authors. 2018
DBID NPM
AAYXX
CITATION
3V.
7X7
7XB
88E
8FI
8FJ
8FK
8G5
ABUWG
AFKRA
AZQEC
BENPR
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
GUQSH
K9.
M0S
M1P
M2O
MBDVC
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOA
DOI 10.3390/ijms19124004
DatabaseName PubMed
CrossRef
ProQuest Central (Corporate)
ProQuest Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
Research Library (Alumni Edition)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
AUTh Library subscriptions: ProQuest Central
ProQuest One Community College
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
Research Library Prep
ProQuest Health & Medical Complete (Alumni)
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
ProQuest_Research Library
Research Library (Corporate)
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle PubMed
CrossRef
Publicly Available Content Database
Research Library Prep
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
Research Library (Alumni Edition)
ProQuest Central China
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
ProQuest Research Library
ProQuest Medical Library (Alumni)
ProQuest Central Basic
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList
MEDLINE - Academic

PubMed
Publicly Available Content Database
CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: AUTh Library subscriptions: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1422-0067
ExternalDocumentID oai_doaj_org_article_23d1d0a5ca6f4040bdc712aa0b5f9c65
10_3390_ijms19124004
30545080
Genre Journal Article
GrantInformation_xml – fundername: Science and Technology Development projects of Jilin Province
  grantid: 20160203005NY
– fundername: National Key Research and Development Program of China
  grantid: 2016YFD0101203)
GroupedDBID ---
29J
2WC
3V.
53G
5GY
5VS
7X7
88E
8FE
8FG
8FH
8FI
8FJ
8G5
A8Z
AADQD
AAFWJ
ABDBF
ABJCF
ABUWG
ACGFO
ACIHN
ACIWK
ACPRK
ADBBV
ADRAZ
AEAQA
AENEX
AFKRA
AFPKN
AFZYC
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
CCPQU
CS3
D1I
DIK
DU5
DWQXO
E3Z
EBD
EBS
EJD
ESTFP
ESX
F5P
FRP
FYUFA
GNUQQ
GROUPED_DOAJ
GUQSH
GX1
HCIFZ
HH5
HMCUK
HYE
IAO
IPNFZ
KB.
KQ8
LK8
M1P
M2O
M48
M7P
MODMG
M~E
NPM
O5R
O5S
OK1
P2P
PDBOC
PIMPY
PQQKQ
PROAC
PSQYO
RIG
RNS
RPM
TR2
TUS
UKHRP
~8M
AAHBH
AAYXX
ALIPV
CITATION
7XB
8FK
K9.
MBDVC
PQEST
PQUKI
PRINS
Q9U
7X8
5PM
ITC
ID FETCH-LOGICAL-c478t-70729b54b892203eddd93f49c51abbbd8b83e749b09cb673ffd58e2cf836beab3
IEDL.DBID RPM
ISSN 1422-0067
1661-6596
IngestDate Fri Jul 05 11:56:59 EDT 2024
Tue Sep 17 21:11:24 EDT 2024
Wed Jul 17 04:19:19 EDT 2024
Fri Sep 13 02:28:45 EDT 2024
Fri Aug 23 00:39:40 EDT 2024
Thu May 23 23:54:10 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 12
Keywords iTRAQ
Zea mays
proteomics
pyruvate biosynthesis
somatic embryogenesis
Language English
License Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c478t-70729b54b892203eddd93f49c51abbbd8b83e749b09cb673ffd58e2cf836beab3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321184/
PMID 30545080
PQID 2582844133
PQPubID 2032341
ParticipantIDs doaj_primary_oai_doaj_org_article_23d1d0a5ca6f4040bdc712aa0b5f9c65
pubmedcentral_primary_oai_pubmedcentral_nih_gov_6321184
proquest_miscellaneous_2157678598
proquest_journals_2582844133
crossref_primary_10_3390_ijms19124004
pubmed_primary_30545080
PublicationCentury 2000
PublicationDate 20181212
PublicationDateYYYYMMDD 2018-12-12
PublicationDate_xml – month: 12
  year: 2018
  text: 20181212
  day: 12
PublicationDecade 2010
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle International journal of molecular sciences
PublicationTitleAlternate Int J Mol Sci
PublicationYear 2018
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References ref13
ref35
ref12
ref34
ref15
ref37
Landi (ref3) 2002; 47
ref14
ref36
ref31
ref30
ref11
ref10
ref32
ref2
ref1
ref17
ref39
ref16
ref38
ref19
ref18
Dennis (ref6) 1991; 10
ref24
ref23
ref26
ref25
ref20
Gilany (ref22) 2011; 2
ref42
ref41
ref21
ref43
ref28
Overvoorde (ref33) 1994; 35
ref27
ref29
ref8
ref7
ref9
ref4
Garrocho-Villegas (ref5) 2012; 877
ref40
References_xml – ident: ref35
  doi: 10.1111/j.1399-3054.2008.01129.x
– ident: ref15
  doi: 10.1002/pmic.200300803
– ident: ref24
  doi: 10.1007/s11103-017-0681-x
– ident: ref29
  doi: 10.1111/j.1440-1835.2004.tb00334.x
– volume: 47
  start-page: 21
  year: 2002
  ident: ref3
  article-title: Responses and allelic frequency changes associated with recurrent selection for plant regeneration from callus cultures in maize
  publication-title: Maydica
  contributor:
    fullname: Landi
– volume: 10
  start-page: 33
  year: 1991
  ident: ref6
  article-title: Somatic embryogenesis and development of synthetic seed technology
  publication-title: Crit. Rev. Plant Sci.
  doi: 10.1080/07352689109382306
  contributor:
    fullname: Dennis
– ident: ref41
  doi: 10.1038/nrm3772
– ident: ref28
  doi: 10.1023/A:1015243600325
– volume: 2
  start-page: 51
  year: 2011
  ident: ref22
  article-title: Proteomics a Key Tool for a Better Understanding of Endometriosis: A Mini-Review
  publication-title: J. Paramed. Sci.
  contributor:
    fullname: Gilany
– ident: ref42
  doi: 10.1016/j.stem.2010.07.011
– ident: ref43
  doi: 10.1016/S0168-9452(00)00382-4
– ident: ref38
  doi: 10.1038/emboj.2011.401
– ident: ref20
  doi: 10.1007/s00299-008-0633-7
– ident: ref21
  doi: 10.1016/j.jprot.2014.03.013
– volume: 35
  start-page: 135
  year: 1994
  ident: ref33
  article-title: The Role of Calcium and Calmodulin in Carrot Somatic Embryogenesis
  publication-title: Plant Cell Physiol.
  contributor:
    fullname: Overvoorde
– ident: ref16
  doi: 10.1007/s00425-006-0238-8
– ident: ref26
  doi: 10.1007/BF00251161
– ident: ref13
  doi: 10.1023/A:1006033622928
– ident: ref40
  doi: 10.3389/fphar.2017.00958
– ident: ref34
  doi: 10.1007/BF02942513
– ident: ref32
  doi: 10.1007/BF00233157
– ident: ref19
  doi: 10.1007/s00299-007-0438-0
– volume: 877
  start-page: 173
  year: 2012
  ident: ref5
  article-title: Maize somatic embryogenesis: Recent features to improve plant regeneration
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-61779-818-4_14
  contributor:
    fullname: Garrocho-Villegas
– ident: ref8
  doi: 10.1016/j.plaphy.2015.04.011
– ident: ref12
  doi: 10.1007/BF00194009
– ident: ref14
  doi: 10.1111/j.1399-3054.2012.01633.x
– ident: ref1
  doi: 10.1126/science.327.5967.797
– ident: ref39
  doi: 10.1016/j.stem.2012.11.022
– ident: ref4
  doi: 10.1038/nbt0386-219
– ident: ref11
  doi: 10.1007/s00299-005-0013-5
– ident: ref30
  doi: 10.3389/fpls.2015.01063
– ident: ref10
  doi: 10.1007/s002990050588
– ident: ref18
  doi: 10.1007/s11240-012-0255-1
– ident: ref23
  doi: 10.1093/jxb/erj168
– ident: ref27
  doi: 10.1038/nbt0696-745
– ident: ref31
  doi: 10.1111/j.1744-7909.2009.00824.x
– ident: ref37
  doi: 10.1111/j.1469-8137.2009.03113.x
– ident: ref36
  doi: 10.1016/j.jprot.2014.02.003
– ident: ref9
  doi: 10.1007/s11240-011-0093-6
– ident: ref17
  doi: 10.1007/s00425-011-1554-1
– ident: ref25
  doi: 10.1371/journal.pone.0119987
– ident: ref7
  doi: 10.1038/nbt.2707
– ident: ref2
  doi: 10.1007/BF00224181
SSID ssj0023259
Score 2.379602
Snippet Somatic embryos (SE) have potential to rapidly form a whole plant. Generally, SE is thought to be derived from embryogenic calli (EC). However, in maize, not...
SourceID doaj
pubmedcentral
proquest
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 4004
SubjectTerms Agricultural biotechnology
Bioinformatics
Biosynthesis
Callus
Cell cycle
Cell division
Embryos
Gluconeogenesis
Glycolysis
Inbreeding
iTRAQ
Metabolism
Peptides
Proteins
proteomics
pyruvate biosynthesis
Pyruvic acid
Signal transduction
somatic embryogenesis
Somatic embryos
Sucrose
Tricarboxylic acid cycle
Zea mays
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1baxQxGA1SEHwR745WiaCgD2Mnt0ny2NaWKrZYaaH6MuRKR7oZ6e4K6y_wZ_tlZnbZFcEXX5MwZPKdJOeQLycIvaw9U4IKW0aqKQgUL0rDtCpVNNSRmkbSe-kdn9RH5_zDhbhYe-or54QN9sDDwO1Q5omvjHCmjhwQZ72ThBpTWRG1qwf3UiKWYmqUWgxY_ZDmzkDU77TfJlMQJjlfkm9sQL1P_9_I5Z85kmubzuEddHtki3h36OVddCOke-jm8H7k4j761Z593j0t92An8vh0blJ_YwzWL_wp2y_kC8d46TqCu4gPJvZ60QFkoNwkj0-6VIa1sn1zddXidwDKH_DBfPMEG3xs2p8Bv_4aDJ6YxRR_fPsGv0-gpD0GIRvwF6BKD9D54cHZ_lE5Pq1QOi7VrJTZMNwKbpWmtGLBe69Z5NoJYqy1XlnFguTaVtrZWrIYvVCBuqhYbYOx7CHaSl0KjxG2RgRhYSYDGLhj0kggodISpqBQBlmgV8vxbr4PDhoNKI8cl2Y9LgXay8FYtcm-130BoKEZ0dD8Cw0F2l6Gshkn47Sh-WgQaB9jBXqxqoZplM9GTArdHNoQEF5SCa0K9GiI_KonsCRy4LFVgeQGJja6ulmT2sveqrtmAE7Fn_yPf3uKbgFbUzmXhtBttDW7nodnwIhm9nkP_t-QGwsj
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Technology Collection
  dbid: 8FG
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELZKERIXxLuBgowEEhzCxnb8yAm1pcuCaEVRKxUukZ8Q1E3KPpCWX8DPZpxkl12EuNpWZGXGM_PZM98g9FQ4pjjlJg20oABQHE81K1SqgqaWCBpIy6V3dCxGZ_m7c36-hUbLWpiYVrm0ia2hdo2Nd-QDGt93wHczNtAm3gLY2eDV5fc09o-K76x9M40r6CqJnHixZnz4ZgW9GG3bphHwRqnghehS4BkA_kH1bTwF0BJzKfMN59Ry-P8r8Pw7f3LNIQ1voht9JIn3OtHfQlu-vo2udb0lF3fQr-r0495Jug9eyuGTua7bajKwbfhDpGaIxch4yUiCm4APx2ayaECdYFzXDh83derXxg70xUWFX4PC_oAPxqoUrPGRrn56_Pyz13isF1P8_uUL_LYGlO0wgFyPP0EYdRedDQ9PD0Zp33YhtblUs1RGMnHDc6MKSjPmnXMFC3lhOdHGGKeMYl7mhckKa4RkITiuPLVBMWG8Nuwe2q6b2u8gbDT33MApB0XJLZNaQoAqDWEKBqWXCXq2_N_lZceuUQIqiXIp1-WSoP0ojNWayIndDjSTL2V_xErKHHGZ5laLkINtMs5KQrXODA-FFTxBu0tRlv1BnZZ_1CpBT1bTcMTiu4mufTOHNQRAmVS8UAm630l-tRMwlznEuFmC5IZObGx1c6auvrY03oIB-Fb5g_9v6yG6DjGaihk0hO6i7dlk7h9BHDQzj1sV_w21WAei
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals (Open Access)
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6VIlAviDcpBRkJJDikbPyInQNCbWlVEFtR1JUKl8iObUi1m8A-EMsv4GczTjarXeiJqx1Hlucbez55_A3A09QyJagwsacZRYJiRaxZpmLlNS2SlPqk0dLrn6THA_7uXJxvQFdtdLGAk0upXagnNRgPd39-n79Gh38VGCdS9pflxWiCtCNkQ_IrcJVyxgPW-3x5n4Bhg8jatPd_RmzBdcQ8F40y5MrZ1Ej4XxZ3_p0-uXIeHd2EG4tAkuy1lr8FG666Ddfa0pLzO_C7PPu4dxrv4yFlyelMV81jMtzayIegzBDeIpNOkITUnhyOzHheI5qwXVeWnNRV7FbaDvRwWJI3iNcf-MPwKIVo0tflL0eef3aajPR8Qt7vviBvKyTZliDHdeQTRlF3YXB0eHZwHC-qLsQFl2oay6AlbgQ3KqO0x5y1NmOeZ4VItDHGKqOYkzwzvawwqWTeW6EcLbxiqXHasHuwWdWVewDEaOGEQSdHnPCCSS1xkaVJmMJG6WQEz7r1zr-14ho5kpJgonzVRBHsB2MsvwmS2E1DPf6SLzwsp8wmtqdFoVPPcWsytpAJ1bpnhM-KVESw05ky72CW03BriBEhYxE8WXajh4VrE125eobfJMjJpBKZiuB-a_nlTDrkRCDXMLE21fWeqvzaqHinDLm34tv_PfIhbGH0pkJuTUJ3YHM6nrlHGCFNzeMG_H8AeNsR9A
  priority: 102
  providerName: Scholars Portal
Title iTRAQ-Based Quantitative Proteomic Analysis of Embryogenic and Non-embryogenic Calli Derived from a Maize ( Zea mays L.) Inbred Line Y423
URI https://www.ncbi.nlm.nih.gov/pubmed/30545080
https://www.proquest.com/docview/2582844133/abstract/
https://search.proquest.com/docview/2157678598
https://pubmed.ncbi.nlm.nih.gov/PMC6321184
https://doaj.org/article/23d1d0a5ca6f4040bdc712aa0b5f9c65
Volume 19
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LjtMwFLVmBiGxQbwnMERGAgkWaRs7jp3ltLQMiFad0YxU2ER-BYKaZNQHUvkCPpvrNKlaxIpNFrYTWb7n2vfE18cIvY4NFYwwFWQkIUBQDAskTUQgMkl0GJMsrLX0xpP44ib6NGOzI8TaszB10r5WeaecF50y_17nVt4WutvmiXWn40FMgbaIqHuMjjmlLUVvWBaFgH6b4U6Bz3fzH8USOIlLlXR38AC8I1aLQO4tQ7Va_79CzL8zJfeWntEDdL-JGfH5tm8P0ZEtH6G721skN4_R7_z66vwy6MN6ZPDlWpb1uTGYxfDUiTC4Y8e41R7BVYaHhVpsKgAOlMvS4ElVBnavbCDn8xy_B2j-hA-68ydY4rHMf1n89quVuJCbJf7ceYc_lsCnDQY6a_EXCJieoJvR8HpwETQXLAQ64mIVcCcbrlikREJIj1pjTEKzKNEslEopI5SglkeJ6iVaxZxmmWHCEp0JGisrFX2KTsqqtKcIK8ksU-DPAIlIUy45DDJXIRVQyC330Jt2vNPbrY5GCvzDmSjdN5GH-s4YuzZO_bouqBbf0gYDKaEmND3JtIyzCGYhZTQPiZQ9xbJEx8xDZ60p08YllylxG4QQ_FHqoVe7anAmt0MiS1utoU0I9IsLlggPPdtafteTFjke4geYOOjqYQ3gtxbsbvD6_L_ffIHuQaAmXBpNSM7QyWqxti8hGFopH1xgxuEpRh98dKc_nEyv_PrHgu8WKQbPcST82kX-AG8rEQM
link.rule.ids 230,315,733,786,790,870,891,2115,2236,12083,12792,21416,24346,27957,27958,31754,31755,33408,33409,33779,33780,43345,43635,43840,53827,53829,74102,74392,74659
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1bb9MwFLZgCMEL4k5ggJFAggezxPc8oW1s6qCtGOqkjpfIjh2WaU1GL0jlF_CzOU7TrkWIV9uKrJzrZx9_B6HX0jEtqLCkoCkFgOIEMSzVRBeG5omkRdJw6fX6snPCPw3FsD1wm7RllUuf2DhqV-fhjHyHhvsdiN2Mfbj8QULXqHC72rbQuI5ucMZ4KOlTwyvAxWjTLC2BGESkSOWi8J0BzN8pz0cTgCqhgpJvhKSGuf9f6ebfVZNrYejwLrrT5o94dyHwe-iar-6jm4uOkvMH6Hc5-Lp7TPYgNjl8PDNV84YMPBr-EggZwhNkvOQhwXWBD0Z2PK9BiWDcVA7364r4tbF9c3FR4o-gpj_hg-EtCja4Z8pfHr_95g0emfkEd9-_w0cVYGuHAdp6fArJ00N0cngw2O-QttkCybnSU6IChbgV3OqU0ph551zKCp7mIjHWWqetZl7x1MZpbqViReGE9jQvNJPWG8seoa2qrvwThK0RXliwbVAPnjNlFKSlyiZMw6DyKkJvlv87u1xwamSARYJcsnW5RGgvCGO1JjBhNwP1-HvWGlZGmUtcbERuZMHBI1mXq4QaE1tRpLkUEdpeijJrzXOSXSlThF6tpsGwwm2JqXw9gzUJQDGlRaoj9Hgh-dVOwElyyGzjCKkNndjY6uZMVZ415N2SAeTW_On_t_US3eoMet2se9T__AzdhixNhxqahG6jrel45p9DJjS1Lxp1_wOc-QbQ
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1bb9MwFLZgE4gXxJ3AACOBBA-hiS-x84TWrdUGW9VNmzR4iXyFoDUZvSCVX8DP5jhNS4sQr8dRZOVcv_j4Owi9yiyVnHAde5ITACiWx4rmMpZeEZNmxKcNl97xIDs4Zx8u-EXb_zRp2yqXMbEJ1LY24R95h4TzHcjdlHZ82xYx3O-_v_oehwlS4aS1HadxHW0LlnGw8O1ubzA8XcEvSprRaSlkpBjWs0UbPAXQ3ym_jSYAXEI_JdtIUA2P_7-Kz797KNeSUv8Out1Wk3h3of676Jqr7qEbi_mS8_voV3l2unsSdyFTWXwyU1VzowziGx4GeoZwIRkvWUlw7XFvpMfzGkwK5KqyeFBXsVuT7anLyxLvg9H-gBeGmylY4WNV_nT4zWen8EjNJ_jo3Vt8WAHSthiArsOfoJR6gM77vbO9g7gdvRAbJuQ0FoFQXHOmZU5IQp21Nqee5YanSmttpZbUCZbrJDc6E9R7y6Ujxkuaaac0fYi2qrpyjxHWijuuwdPBWJihQgkoUoVOqQShcCJCr5ffu7haMGwUgEyCXop1vUSoG5SxeibwYjeCevylaN2sINSmNlHcqMwziE_aGpESpRLNfW4yHqGdpSqL1lknxR_TitDL1TK4WTg7UZWrZ_BMCsBMSJ7LCD1aaH61EwiZDOrcJEJiwyY2trq5UpVfGyrvjAIAl-zJ_7f1At0EWy-ODgcfn6JbULLJ0FCTkh20NR3P3DMoi6b6eWvvvwF6sAxz
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=iTRAQ-Based+Quantitative+Proteomic+Analysis+of+Embryogenic+and+Non-embryogenic+Calli+Derived+from+a+Maize+%28Zea+mays+L.%29+Inbred+Line+Y423&rft.jtitle=International+journal+of+molecular+sciences&rft.au=Liu%2C+Beibei&rft.au=Shan%2C+Xiaohui&rft.au=Wu%2C+Ying&rft.au=Su%2C+Shengzhong&rft.date=2018-12-12&rft.pub=MDPI&rft.eissn=1422-0067&rft.volume=19&rft.issue=12&rft_id=info:doi/10.3390%2Fijms19124004&rft_id=info%3Apmid%2F30545080&rft.externalDBID=PMC6321184
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1422-0067&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1422-0067&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1422-0067&client=summon