Splice Variants of the Castor WRI1 Gene Upregulate Fatty Acid and Oil Biosynthesis When Expressed in Tobacco Leaves
The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean ( L.), an important industrial oil crop. Here, we repor...
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Published in | International journal of molecular sciences Vol. 19; no. 1; p. 146 |
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Main Authors | , , , , , |
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Language | English |
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Abstract | The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean (
L.), an important industrial oil crop. Here, we report the identification of two alternatively spliced transcripts of
, designated as
and
. The open reading frames of
(1341 bp) and
(1332 bp) differ by a stretch of 9 bp, such that the predicted RcWRI1-B lacks the three amino acid residues "VYL" that are present in RcWRI1-A. The
transcript is present in flowers, leaves, pericarps and developing seeds, while the
mRNA is only detectable in developing seeds. When the two isoforms were individually introduced into an
loss-of-function mutant, total fatty acid content was almost restored to the wild-type level, and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the
mutant line. Transient expression of each
splice isoform in
leaves upregulated the expression of the WRI1 target genes, and consequently increased the oil content by 4.3-4.9 fold when compared with the controls, and
appeared to be more active than
. Both
and
can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass. |
---|---|
AbstractList | The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean (Ricinus connunis L.), an important industrial oil crop. Here, we report the identification of two alternatively spliced transcripts of RcWRI1, designated as RcWRI1-A and RcWRI1-B. The open reading frames of RcWRI1-A (1341 bp) and RcWRI1-B (1332 bp) differ by a stretch of 9 bp, such that the predicted RcWRI1-B lacks the three amino acid residues “VYL” that are present in RcWRI1-A. The RcWRI1-A transcript is present in flowers, leaves, pericarps and developing seeds, while the RcWRI1-B mRNA is only detectable in developing seeds. When the two isoforms were individually introduced into an Arabidopsis wri1-1 loss-of-function mutant, total fatty acid content was almost restored to the wild-type level, and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the wri1-1 mutant line. Transient expression of each RcWRI1 splice isoform in N. benthamiana leaves upregulated the expression of the WRI1 target genes, and consequently increased the oil content by 4.3–4.9 fold when compared with the controls, and RcWRI1-B appeared to be more active than RcWRI1-A. Both RcWRI1-A and RcWRI1-B can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass. The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean (Ricinus connunis L.), an important industrial oil crop. Here, we report the identification of two alternatively spliced transcripts of RcWRI1, designated as RcWRI1-A and RcWRI1-B. The open reading frames of RcWRI1-A (1341 bp) and RcWRI1-B (1332 bp) differ by a stretch of 9 bp, such that the predicted RcWRI1-B lacks the three amino acid residues "VYL" that are present in RcWRI1-A. The RcWRI1-A transcript is present in flowers, leaves, pericarps and developing seeds, while the RcWRI1-B mRNA is only detectable in developing seeds. When the two isoforms were individually introduced into an Arabidopsiswri1-1 loss-of-function mutant, total fatty acid content was almost restored to the wild-type level, and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the wri1-1 mutant line. Transient expression of each RcWRI1 splice isoform in N. benthamiana leaves upregulated the expression of the WRI1 target genes, and consequently increased the oil content by 4.3-4.9 fold when compared with the controls, and RcWRI1-B appeared to be more active than RcWRI1-A. Both RcWRI1-A and RcWRI1-B can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass. The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean ( Ricinus connunis L.), an important industrial oil crop. Here, we report the identification of two alternatively spliced transcripts of RcWRI1 , designated as RcWRI1-A and RcWRI1-B . The open reading frames of RcWRI1-A (1341 bp) and RcWRI1-B (1332 bp) differ by a stretch of 9 bp, such that the predicted RcWRI1-B lacks the three amino acid residues “VYL” that are present in RcWRI1-A. The RcWRI1-A transcript is present in flowers, leaves, pericarps and developing seeds, while the RcWRI1-B mRNA is only detectable in developing seeds. When the two isoforms were individually introduced into an Arabidopsis wri1-1 loss-of-function mutant, total fatty acid content was almost restored to the wild-type level, and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the wri1-1 mutant line. Transient expression of each RcWRI1 splice isoform in N. benthamiana leaves upregulated the expression of the WRI1 target genes, and consequently increased the oil content by 4.3–4.9 fold when compared with the controls, and RcWRI1-B appeared to be more active than RcWRI1-A . Both RcWRI1-A and RcWRI1-B can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass. The plant-specific WRINKLED1 (WRI1) is a member of the AP2/EREBP class of transcription factors that positively regulate oil biosynthesis in plant tissues. Limited information is available for the role of WRI1 in oil biosynthesis in castor bean ( L.), an important industrial oil crop. Here, we report the identification of two alternatively spliced transcripts of , designated as and . The open reading frames of (1341 bp) and (1332 bp) differ by a stretch of 9 bp, such that the predicted RcWRI1-B lacks the three amino acid residues "VYL" that are present in RcWRI1-A. The transcript is present in flowers, leaves, pericarps and developing seeds, while the mRNA is only detectable in developing seeds. When the two isoforms were individually introduced into an loss-of-function mutant, total fatty acid content was almost restored to the wild-type level, and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the mutant line. Transient expression of each splice isoform in leaves upregulated the expression of the WRI1 target genes, and consequently increased the oil content by 4.3-4.9 fold when compared with the controls, and appeared to be more active than . Both and can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass. |
Author | Liu, Bao-Ling Xue, Jin-Ai Mao, Xue Hao, Qing-Ting Ji, Xia-Jie Li, Run-Zhi |
AuthorAffiliation | Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China; jixiajie@stu.sxau.edu.cn (X.-J.J.); maoxue@sxau.edu.cn (X.M.); haoqingting@stu.sxau.edu.cn (Q.-T.H.); liubaoling@stu.sxau.edu.cn (B.-L.L.); xuejinai@sxau.edu.cn (J.-A.X.) |
AuthorAffiliation_xml | – name: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China; jixiajie@stu.sxau.edu.cn (X.-J.J.); maoxue@sxau.edu.cn (X.M.); haoqingting@stu.sxau.edu.cn (Q.-T.H.); liubaoling@stu.sxau.edu.cn (B.-L.L.); xuejinai@sxau.edu.cn (J.-A.X.) |
Author_xml | – sequence: 1 givenname: Xia-Jie surname: Ji fullname: Ji, Xia-Jie email: jixiajie@stu.sxau.edu.cn organization: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China. jixiajie@stu.sxau.edu.cn – sequence: 2 givenname: Xue surname: Mao fullname: Mao, Xue email: maoxue@sxau.edu.cn organization: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China. maoxue@sxau.edu.cn – sequence: 3 givenname: Qing-Ting surname: Hao fullname: Hao, Qing-Ting email: haoqingting@stu.sxau.edu.cn organization: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China. haoqingting@stu.sxau.edu.cn – sequence: 4 givenname: Bao-Ling surname: Liu fullname: Liu, Bao-Ling email: liubaoling@stu.sxau.edu.cn organization: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China. liubaoling@stu.sxau.edu.cn – sequence: 5 givenname: Jin-Ai surname: Xue fullname: Xue, Jin-Ai email: xuejinai@sxau.edu.cn organization: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China. xuejinai@sxau.edu.cn – sequence: 6 givenname: Run-Zhi surname: Li fullname: Li, Run-Zhi email: lirunzhi@sxau.edu.cn organization: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu 030801, China. lirunzhi@sxau.edu.cn |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29303957$$D View this record in MEDLINE/PubMed |
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Keywords | tobacco (Nicotiana benthamiana L.) alternative splice form castor (Ricinus communis L.) RcWRI1 fatty acid and oil biosynthesis |
Language | English |
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SubjectTerms | alternative splice form Alternative Splicing Biosynthesis castor (Ricinus communis L.) Castor Oil - biosynthesis Castor Oil - genetics fatty acid and oil biosynthesis Fatty acids Fatty Acids - biosynthesis Fatty Acids - genetics Flowers Gene expression Gene Expression Regulation, Plant Isoforms Leaves Nicotiana - genetics Nicotiana - metabolism Nicotiana tabacum Oilseeds Open reading frames Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism RcWRI1 Ricinus - genetics Ricinus communis Seeds Tobacco tobacco (Nicotiana benthamiana L.) Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transgenes Up-Regulation |
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Title | Splice Variants of the Castor WRI1 Gene Upregulate Fatty Acid and Oil Biosynthesis When Expressed in Tobacco Leaves |
URI | https://www.ncbi.nlm.nih.gov/pubmed/29303957 https://www.proquest.com/docview/2109459278/abstract/ https://search.proquest.com/docview/1989537600 https://pubmed.ncbi.nlm.nih.gov/PMC5796095 https://doaj.org/article/512af52e6a2a407e95070e5e8df76351 |
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