' Candidatus Liberibacter Asiaticus' SDE1 Effector Induces Huanglongbing Chlorosis by Downregulating Host DDX3 Gene

• Published in: International journal of molecular sciences Vol. 21; no. 21; p. 7996
• Main Authors: Zhou, Yinghui, Wei, Xiangying, Li, Yanjiao, Liu, Zhiqin, Duan, Yongping, Zou, Huasong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.10.2020; MDPI
• Subjects: Amino acids; Apoptosis; Bacterial Proteins - metabolism; Candidatus Liberibacter asiaticus; Cell death; Cell Membrane - metabolism; Cell membranes; Chlorosis; Citrus - genetics; Citrus - metabolism; Citrus - microbiology; Citrus fruits; Cloning; CsDDX3; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; DNA helicase; Effectors; Gene expression; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Plant; Gene Silencing; Genetic modification; Genome editing; Genotype & phenotype; Leaves; Liberibacter - metabolism; Liberibacter - pathogenicity; Mimicry; NbDDX3; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - microbiology; Nicotiana benthamiana; Pathogens; Plant bacterial diseases; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - microbiology; Plant Necrosis and Chlorosis - genetics; Plant Necrosis and Chlorosis - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Binding; Proteins; RNA helicase; RNA-Dependent RNA Polymerase - metabolism; SDE1; Yellowing; NbDDX3; Candidatus Liberibacter asiaticus; CsDDX3; SDE1; chlorosis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21217996

' Liberibacter asiaticus' (CLas) is the pathogenic bacterium that causes the disease Huanglongbing (HLB) in citrus and some model plants, such as . After infection, CLas releases a set of effectors to modulate host responses. One of these critical effectors is Sec-delivered effector 1 (SDE1), which induces chlorosis and cell death in . In this study, we revealed the DEAD-box RNA helicase (DDX3) interacts with SDE1. Gene silencing study revealed that knockdown of the gene triggers leaf chlorosis, mimicking the primary symptom of CLas infection in . The interactions between SDE1 and NbDDX3 were localized in the cell membrane. Overexpression of resulted in suppression of gene expression in , which suggests a critical role of in modulating expression. Furthermore, we verified the interaction of SDE1 with citrus DDX3 (CsDDX3), and demonstrated that the expression of the gene was significantly reduced in HLB-affected yellowing and mottled leaves of citrus. Thus, we provide molecular evidence that the downregulation of the host gene is a crucial mechanism of leaf chlorosis in HLB-affected plants. The identification of CsDDX3 as a critical target of SDE1 and its association with HLB symptom development indicates that the gene is an important target for gene editing, to interrupt the interaction between DDX3 and SDE1, and therefore interfere host susceptibility.