Human Plasminogen Exacerbates Clostridioides difficile Enteric Disease and Alters the Spore Surface

• Published in: Gastroenterology (New York, N.Y. 1943) Vol. 159; no. 4; pp. 1431 - 1443.e6
• Main Authors: Awad, Milena M., Hutton, Melanie L., Quek, Adam J., Klare, William P., Mileto, Steven J., Mackin, Kate, Ly, Diane, Oorschot, Viola, Bosnjak, Marijana, Jenkin, Grant, Conroy, Paul J., West, Nick, Fulcher, Alex, Costin, Adam, Day, Christopher J., Jennings, Michael P., Medcalf, Robert L., Sanderson-Smith, Martina, Cordwell, Stuart J., Law, Ruby H.P., Whisstock, James C., Lyras, Dena
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2020
• Subjects: Animals; Clostridioides difficile - drug effects; Disease Models, Animal; Enterocolitis, Pseudomembranous - etiology; Enterocolitis, Pseudomembranous - pathology; Fibrinolytic System; Humans; Intestine, Small; Liver Enzyme; Mice; Mice, Inbred C57BL; Microbe; Pathogenic Bacteria; Plasminogen - pharmacology; Spores, Bacterial - drug effects; PBS; TBS; tPA; Pathogenic Bacteria; Fibrinolytic System; STED; uPA; CDI; BMDM; mPLG; Liver Enzyme; ECM; huPA; LC-MS/MS; Microbe; hPLG; WT

The protease plasmin is an important wound healing factor, but it is not clear how it affects gastrointestinal infection–mediated damage, such as that resulting from Clostridioides difficile. We investigated the role of plasmin in C difficile–associated disease. This bacterium produces a spore form...