Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G - Lin, Baixue; Fan, Keqiang; Zhao, Jian; Ji, Junjie; Wu, Linjun; Yang, Keqian; Tao, Yong (Proceedings of the National Academy of Sciences - PNAS, 2015)
Keywords: Acetates - metabolism; Biocatalysis - drug effects; Biochemistry; Biological Sciences; Catalysis; Cephalosporins - chemistry; Cephalosporins - metabolism; Chemical pollution; Chemical reactions; Citric Acid Cycle - drug effects; E coli; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Knockout Techniques; Genes, Bacterial; Glucose - pharmacology; Glyoxylates - metabolism; Intramolecular Transferases - metabolism; Metabolic Engineering - methods; Mutation - genetics; Organic chemicals; Penicillin; Penicillin G - chemistry; Penicillin G - metabolism; Penicillin-Binding Proteins - metabolism; Streptomyces - drug effects; Streptomyces - enzymology; Time Factors; reconstitution; whole-cell catalyst; G-7-ADCA; TCA cycle; DAOCS