Salmonella Infection Drives Promiscuous B Cell Activation Followed by Extrafollicular Affinity Maturation

• Published in: Immunity (Cambridge, Mass.) Vol. 43; no. 1; pp. 120 - 131
• Main Authors: Di Niro, Roberto, Lee, Seung-Joo, Vander Heiden, Jason A., Elsner, Rebecca A., Trivedi, Nikita, Bannock, Jason M., Gupta, Namita T., Kleinstein, Steven H., Vigneault, Francois, Gilbert, Tamara J., Meffre, Eric, McSorley, Stephen J., Shlomchik, Mark J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.07.2015; Elsevier Limited
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antigens; B-Lymphocytes - immunology; Clonal Selection, Antigen-Mediated - genetics; Clonal Selection, Antigen-Mediated - immunology; Experiments; Germinal Center - immunology; Immunoglobulin G - biosynthesis; Immunoglobulin G - immunology; Infections; Lymphocyte Activation - immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Mutation; Receptors, Antigen, B-Cell - genetics; Receptors, Antigen, B-Cell - immunology; Salmonella; Salmonella Infections - immunology; Salmonella Infections - microbiology; Salmonella typhimurium; Salmonella typhimurium - immunology; Somatic Hypermutation, Immunoglobulin - genetics; Somatic Hypermutation, Immunoglobulin - immunology; Spleen - cytology; Spleen - immunology; T cell receptors

The B cell response to Salmonella typhimurium (STm) occurs massively at extrafollicular sites, without notable germinal centers (GCs). Little is known in terms of its specificity. To expand the knowledge of antigen targets, we screened plasmablast (PB)-derived monoclonal antibodies (mAbs) for Salmon...