ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells

• Published in: Nucleic acids research Vol. 44; no. 17; pp. 8165 - 8178
• Main Authors: Riso, Vincenzo, Cammisa, Marco, Kukreja, Harpreet, Anvar, Zahra, Verde, Gaetano, Sparago, Angela, Acurzio, Basilia, Lad, Shraddha, Lonardo, Enza, Sankar, Aditya, Helin, Kristian, Feil, Robert, Fico, Annalisa, Angelini, Claudia, Grimaldi, Giovanna, Riccio, Andrea
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 30.09.2016
• Subjects: Animals; Binding Sites - genetics; Biochemistry, Molecular Biology; Cell Differentiation - genetics; CpG Islands - genetics; Development Biology; Epigenesis, Genetic; Gene Expression Regulation, Developmental; Gene regulation, Chromatin and Epigenetics; Genetic Loci; Genetics; Genomic Imprinting; Histones - metabolism; Life Sciences; Lysine - metabolism; Methylation; Mice; Models, Genetic; Mouse Embryonic Stem Cells - metabolism; Nuclear Proteins - metabolism; Repressor Proteins - metabolism

ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-lo...