Generation of trophoblast-like cells from the amnion in vitro: A novel cellular model for trophoblast development

• Published in: Placenta (Eastbourne) Vol. 51; pp. 28 - 37
• Main Authors: Wei, Yanxing, Zhou, Xiaohua, Huang, Wenhao, Long, Ping, Xiao, Lu, Zhang, Tian, Zhong, Mei, Pan, Guangjin, Ma, Yanlin, Yu, Yanhong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.03.2017
• Subjects: Adult; Amnion; Amnion - cytology; Amnion - drug effects; Amnion - metabolism; BMP4; Bone Morphogenetic Protein 4 - pharmacology; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cell Line; Differentiation; Female; Humans; Induced pluripotent stem cell; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - drug effects; Induced Pluripotent Stem Cells - metabolism; Internal Medicine; Obstetrics and Gynecology; Pregnancy; Transcriptome - drug effects; Trophoblast; Trophoblasts - cytology; Trophoblasts - drug effects; Trophoblasts - metabolism; BMP4; Amnion; Induced pluripotent stem cell; Trophoblast; Differentiation

Despite the high incidence of trophoblast-related diseases, the molecular mechanism of inadequate early trophoblast development is still unclear due to the lack of an appropriate cellular model in vitro. In the present study, we reprogrammed the amniotic cells to be induced pluripotent stem cells (i...