Lipid changes after leaf wounding in Arabidopsis thaliana: expanded lipidomic data form the basis for lipid co‐occurrence analysis

• Published in: The Plant journal : for cell and molecular biology Vol. 80; no. 4; pp. 728 - 743
• Main Authors: Vu, Hieu Sy, Shiva, Sunitha, Roth, Mary R, Tamura, Pamela, Zheng, Lianqing, Li, Maoyin, Sarowar, Sujon, Honey, Samuel, McEllhiney, Dedan, Hinkes, Paul, Seib, Lawrence, Williams, Todd D, Gadbury, Gary, Wang, Xuemin, Shah, Jyoti, Welti, Ruth
• Format: Journal Article
• Language: English
• Published: England Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology 01.11.2014; Blackwell Publishing Ltd
• Subjects: acids; Arabidopsis - metabolism; Arabidopsis thaliana; dendrogram; direct‐infusion triple–quadrupole mass spectrometry; Enzymes; galactolipids; Galactolipids - analysis; Galactolipids - metabolism; glycerolipids; ionization; leaf wounding; Leaves; lipid clustering; lipidomics; Lipids; Lipids - analysis; Lipids - chemistry; Mass spectrometry; metabolism; monitoring; oxidized lipids; Phosphatidic Acids - analysis; Phosphatidic Acids - metabolism; phosphatidylglycerols; Phospholipids - analysis; Plant biology; Plant Leaves - chemistry; Plant Leaves - metabolism; Spearman's correlation; Spectrometry, Mass, Electrospray Ionization - methods; sphingolipids; sterol derivatives; Sterols; technical advance; Arabidopsis thaliana; glycerolipids; lipid clustering; sterol derivatives; direct-infusion triple-quadrupole mass spectrometry; technical advance; Spearman's correlation; leaf wounding; oxidized lipids; dendrogram; lipidomics

A direct‐infusion electrospray ionization triple–quadrupole mass spectrometry method with multiple reaction monitoring (MRM) was employed to measure 264 lipid analytes extracted from leaves of Arabidopsis thaliana subjected to mechanical wounding. The method provided precise measurements with an ave...