Identification of gel-separated tumor marker proteins by mass spectrometry

• Published in: Electrophoresis Vol. 21; no. 3; pp. 679 - 686
• Main Authors: Bergman, Ann-Charlotte, Benjamin, Timothy, Alaiya, Ayodele, Waltham, Mark, Sakaguchi, Kazuyazu, Franzén, Bo, Linder, Stig, Bergman, Tomas, Auer, Gert, Appella, Ettore, Wirth, Peter J., Jörnvall, Hans
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.02.2000
• Subjects: Biomarkers, Tumor - analysis; Blotting, Western; Breast Neoplasms - chemistry; Cell Extracts - chemistry; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Lung Neoplasms - chemistry; Mass spectrometry; Mass Spectrometry - methods; Ovarian Neoplasms - chemistry; Tumor markers; Two-dimensional gel electrophoresis

Two‐dimensional gel electrophoresis with subsequent analysis by mass spectrometry was applied to study differences in protein expression between benign and malignant solid tumors from human beast, lung and ovary cells. Cells from freshly resected clinical material were lysed and the extracts were su...