Co‐culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells

• Published in: Journal of cellular and molecular medicine Vol. 14; no. 1‐2; pp. 337 - 350
• Main Authors: Walenda, Thomas, Bork, Simone, Horn, Patrick, Wein, Frederik, Saffrich, Rainer, Diehlmann, Anke, Eckstein, Volker, Ho, Anthony D., Wagner, Wolfgang
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.2010; John Wiley & Sons, Inc
• Subjects: adhesion proteins; ADP-ribosyl Cyclase 1 - metabolism; Antibodies; Antigens, CD34 - metabolism; Biotechnology; Carboxyfluorescein diacetate; CD13 antigen; CD34 antigen; CD38 antigen; CD44 antigen; CD45 antigen; CD56 antigen; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell culture; Cell Culture Techniques; Cell differentiation; Cell Differentiation - physiology; Cell division; Cell Proliferation; Cell self-renewal; Cells, Cultured; Cellular Senescence - physiology; Cloning; Coculture Techniques; Cord blood; co‐culture; Fetal Blood - cytology; Flow cytometry; Genetics; Genotype & phenotype; haematopoietic stem cells; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - physiology; Hemopoiesis; Human subjects; Humans; immunophenotype; Immunophenotyping; Kinases; Laboratories; MAP kinase; Mesenchymal stem cells; mesenchymal stromal cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Mitogen-Activated Protein Kinase 1 - antagonists & inhibitors; Mitogen-Activated Protein Kinase 1 - genetics; Mitogen-Activated Protein Kinase 1 - metabolism; N-Cadherin; Progenitor cells; proliferation; Proteins; replicative senescence; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; siRNA; stem cell niche; Stem cells; Stromal cells; Stromal Cells - cytology; Stromal Cells - physiology; Studies; Umbilical cord; United States > US; Germany

Mesenchymal stromal cells (MSC) have been suggested to provide a suitable cellular environment for in vitro expansion of haematopoietic stem and progenitor cells (HPC) from umbilical cord blood. In this study, we have simultaneously analysed the cell division history and immunophenotypic differentia...