DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos

• Published in: Development (Cambridge) Vol. 142; no. 3; pp. 510 - 521
• Main Authors: Jacob, Vinitha, Chernyavskaya, Yelena, Chen, Xintong, Tan, Poh Seng, Kent, Brandon, Hoshida, Yujin, Sadler, Kirsten C.
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists 01.02.2015
• Subjects: Animals; Apoptosis - physiology; Bromodeoxyuridine; Cell Cycle Checkpoints - physiology; Danio rerio; DNA Methylation - physiology; DNA Replication - physiology; Embryo, Nonmammalian - physiology; Epigenesis, Genetic - physiology; Gene Expression Profiling; In Situ Nick-End Labeling; Liver - embryology; Statistics, Nonparametric; Trans-Activators - genetics; Zebrafish - embryology; Zebrafish - genetics; Zebrafish Proteins - genetics; DNA methylation; Hepatic outgrowth; Zebrafish; DNA replication; UHRF1; Liver development

UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is essential for maintaining DNA methylation. uhrf1 mutant zebrafish have global DNA hypomethylation and display embryonic defects, including a small liver, and they die as l...