RNA Interference Screen to Identify Pathways That Enhance or Reduce Nonviral Gene Transfer During Lipofection

• Published in: Molecular therapy Vol. 16; no. 9; pp. 1602 - 1608
• Main Authors: Barker, Gregory A, Diamond, Scott L
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2008; Elsevier Limited
• Subjects: Aorta - cytology; Aorta - metabolism; Candidates; Cells, Cultured; Efficiency; Electroporation; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Flow Cytometry; Gene Silencing; Gene therapy; Gene Transfer Techniques; Genetic Vectors - genetics; Genetic Vectors - pharmacology; Genome, Human; Genomes; Humans; Lipids - chemistry; Luciferases - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA, Messenger - antagonists & inhibitors; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - pharmacology; Signal Transduction; Transfection; United States > US; Pennsylvania

Some barriers to DNA lipofection are well characterized; however, there is as yet no method of finding unknown pathways that impact the process. A druggable genome small-interfering RNA (siRNA) screen against 5,520 genes was tested for its effect on lipofection of human aortic endothelial cells (HAE...