Microbial community of a mesophilic propionate-degrading methanogenic consortium in chemostat cultivation analyzed based on 16S rRNA and acetate kinase genes

• Published in: Applied microbiology and biotechnology Vol. 72; no. 2; pp. 401 - 415
• Main Authors: Shigematsu, Toru, Era, Shinobu, Mizuno, Yuko, Ninomiya, Kana, Kamegawa, Yukiko, Morimura, Shigeru, Kida, Kenji
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01.09.2006; Springer; Springer Nature B.V
• Subjects: Acetate Kinase - genetics; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biological and medical sciences; Bioreactors - microbiology; Biotechnology; Cloning; Euryarchaeota - classification; Euryarchaeota - genetics; Euryarchaeota - metabolism; Firmicutes; Fundamental and applied biological sciences. Psychology; Genes; In Situ Hybridization, Fluorescence; Kinases; Methanoculleus; Methanosaeta; Microbiology; Microscopy, Phase-Contrast - methods; Molecular Sequence Data; Phylogeny; Propionates - metabolism; RNA, Archaeal - genetics; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Enzyme; Transferases; Degradation; Bioreactor; Gene; Ribosomal RNA; Mesophily; Methanogenesis; 16S-RNA; Acetate kinase; Chemostat; Microbial community; Culture

We constructed a mesophilic anaerobic chemostat that was continuously fed with synthetic wastewater containing propionate as the sole source of carbon and energy. Steady-state conditions were achieved below the critical dilution rate of 0.3 d -¹ with almost complete substrate degradation. The propio...