En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs

• Published in: Nucleic acids research Vol. 40; no. 20; pp. 10394 - 10407
• Main Authors: Masuda, Yuji, Suzuki, Miki, Kawai, Hidehiko, Hishiki, Asami, Hashimoto, Hiroshi, Masutani, Chikahide, Hishida, Takashi, Suzuki, Fumio, Kamiya, Kenji
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.11.2012
• Subjects: DNA biosynthesis; DNA repair; DNA-Binding Proteins - metabolism; Humans; Nucleic Acid Enzymes; Polyubiquitin - metabolism; Post-replication; Proliferating cell nuclear antigen; Proliferating Cell Nuclear Antigen - metabolism; Replication; Transcription Factors - metabolism; Ubiquitin; Ubiquitin-Conjugating Enzymes - metabolism; Ubiquitin-protein ligase; Ubiquitin-Protein Ligases - metabolism; Ubiquitination

Post-replication DNA repair in eukaryotes is regulated by ubiquitination of proliferating cell nuclear antigen (PCNA). Monoubiquitination catalyzed by RAD6-RAD18 (an E2-E3 complex) stimulates translesion DNA synthesis, whereas polyubiquitination, promoted by additional factors such as MMS2-UBC13 (a...