Isolation of Hyperactive Mutants of Mammalian Target of Rapamycin

• Published in: The Journal of biological chemistry Vol. 283; no. 46; pp. 31861 - 31870
• Main Authors: Ohne, Yoichiro, Takahara, Terunao, Hatakeyama, Riko, Matsuzaki, Tomoko, Noda, Makoto, Mizushima, Noboru, Maeda, Tatsuya
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.11.2008; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Autophagy; Cell Line; Cell Size; Enzyme Activation; Gene Expression Regulation, Enzymologic; Humans; Mechanistic Target of Rapamycin Complex 1; Mice; Multiprotein Complexes; Mutation - genetics; Protein Kinases - genetics; Protein Kinases - metabolism; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Signal Transduction; TOR Serine-Threonine Kinases; Transcription Factors - metabolism

The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as protein synthesis, cell sizing, and autophagy. mTOR forms two functionally distinct complexes, termed the mTOR complex 1 (mTORC1) and 2 (mTORC...