Isolation of Hyperactive Mutants of Mammalian Target of Rapamycin

The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as protein synthesis, cell sizing, and autophagy. mTOR forms two functionally distinct complexes, termed the mTOR complex 1 (mTORC1) and 2 (mTORC...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of biological chemistry Vol. 283; no. 46; pp. 31861 - 31870
Main Authors Ohne, Yoichiro, Takahara, Terunao, Hatakeyama, Riko, Matsuzaki, Tomoko, Noda, Makoto, Mizushima, Noboru, Maeda, Tatsuya
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.11.2008
American Society for Biochemistry and Molecular Biology
Subjects
Online AccessGet full text

Cover

Loading…
Abstract The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as protein synthesis, cell sizing, and autophagy. mTOR forms two functionally distinct complexes, termed the mTOR complex 1 (mTORC1) and 2 (mTORC2); only the former of which is inhibited by rapamycin. Based on the similarity between the cellular responses caused by rapamycin treatment and by nutrient starvation, it has been widely accepted that modulation in the mTORC1 activity in response to nutrient status directs these cellular responses, although direct evidence has been scarce. Here we report isolation of hyperactive mutants of mTOR. The isolated mTOR mutants exhibited enhanced kinase activity in vitro and rendered cells refractory to the dephosphorylation of the mTORC1 substrates upon amino acid starvation. Cells expressing the hyperactive mTOR mutant displayed larger cell size in a normal growing condition and were resistant to cell size reduction and autophagy induction in an amino acid-starved condition. These results indicate that the activity of mTORC1 actually directs these cellular processes in response to nutrient status and confirm the biological functions of mTORC1, which had been proposed solely from loss-of-function analyses using rapamycin and (molecular)genetic techniques. Additionally, the hyperactive mTOR mutant did not induce cellular transformation of NIH/3T3 cells, suggesting that concomitant activation of additional pathways is required for tumorigenesis. This hyperactive mTOR mutant will be a valuable tool for establishing physiological consequences of mTOR activation in cells as well as in organisms.
AbstractList The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as protein synthesis, cell sizing, and autophagy. mTOR forms two functionally distinct complexes, termed the mTOR complex 1 (mTORC1) and 2 (mTORC2); only the former of which is inhibited by rapamycin. Based on the similarity between the cellular responses caused by rapamycin treatment and by nutrient starvation, it has been widely accepted that modulation in the mTORC1 activity in response to nutrient status directs these cellular responses, although direct evidence has been scarce. Here we report isolation of hyperactive mutants of mTOR. The isolated mTOR mutants exhibited enhanced kinase activity in vitro and rendered cells refractory to the dephosphorylation of the mTORC1 substrates upon amino acid starvation. Cells expressing the hyperactive mTOR mutant displayed larger cell size in a normal growing condition and were resistant to cell size reduction and autophagy induction in an amino acid-starved condition. These results indicate that the activity of mTORC1 actually directs these cellular processes in response to nutrient status and confirm the biological functions of mTORC1, which had been proposed solely from loss-of-function analyses using rapamycin and (molecular)genetic techniques. Additionally, the hyperactive mTOR mutant did not induce cellular transformation of NIH/3T3 cells, suggesting that concomitant activation of additional pathways is required for tumorigenesis. This hyperactive mTOR mutant will be a valuable tool for establishing physiological consequences of mTOR activation in cells as well as in organisms.
The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as protein synthesis, cell sizing, and autophagy. mTOR forms two functionally distinct complexes, termed the mTOR complex 1 (mTORC1) and 2 (mTORC2); only the former of which is inhibited by rapamycin. Based on the similarity between the cellular responses caused by rapamycin treatment and by nutrient starvation, it has been widely accepted that modulation in the mTORC1 activity in response to nutrient status directs these cellular responses, although direct evidence has been scarce. Here we report isolation of hyperactive mutants of mTOR. The isolated mTOR mutants exhibited enhanced kinase activity in vitro and rendered cells refractory to the dephosphorylation of the mTORC1 substrates upon amino acid starvation. Cells expressing the hyperactive mTOR mutant displayed larger cell size in a normal growing condition and were resistant to cell size reduction and autophagy induction in an amino acid-starved condition. These results indicate that the activity of mTORC1 actually directs these cellular processes in response to nutrient status and confirm the biological functions of mTORC1, which had been proposed solely from loss-of-function analyses using rapamycin and (molecular)genetic techniques. Additionally, the hyperactive mTOR mutant did not induce cellular transformation of NIH/3T3 cells, suggesting that concomitant activation of additional pathways is required for tumorigenesis. This hyperactive mTOR mutant will be a valuable tool for establishing physiological consequences of mTOR activation in cells as well as in organisms.
Author Hatakeyama, Riko
Maeda, Tatsuya
Noda, Makoto
Mizushima, Noboru
Ohne, Yoichiro
Takahara, Terunao
Matsuzaki, Tomoko
Author_xml – sequence: 1
  givenname: Yoichiro
  surname: Ohne
  fullname: Ohne, Yoichiro
  organization: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
– sequence: 2
  givenname: Terunao
  surname: Takahara
  fullname: Takahara, Terunao
  organization: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
– sequence: 3
  givenname: Riko
  surname: Hatakeyama
  fullname: Hatakeyama, Riko
  organization: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
– sequence: 4
  givenname: Tomoko
  surname: Matsuzaki
  fullname: Matsuzaki, Tomoko
  organization: Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
– sequence: 5
  givenname: Makoto
  surname: Noda
  fullname: Noda, Makoto
  organization: Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
– sequence: 6
  givenname: Noboru
  surname: Mizushima
  fullname: Mizushima, Noboru
  organization: Department of Physiology and Cell Biology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan
– sequence: 7
  givenname: Tatsuya
  surname: Maeda
  fullname: Maeda, Tatsuya
  email: maeda@iam.u-tokyo.ac.jp
  organization: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
BackLink https://www.ncbi.nlm.nih.gov/pubmed/18812319$$D View this record in MEDLINE/PubMed
BookMark eNp1kE1LAzEQhoMoWqtXj9KDeNuabHaT7FHEL2gRpIK3MMnO2sjupiZbpf_elBY8OZeBmecdhueUHPa-R0IuGJ0yKoubT2Onc0VZWYic0gMyYlTxjJfs_ZCMKM1ZVuWlOiGnMX7SVEXFjskJU4rlnFUjcvscfQuD8_3EN5OnzQoD2MF942S-HqAf4nY8h66D1kE_WUD4wGE7e4UVdBvr-jNy1EAb8Xzfx-Tt4X5x95TNXh6f725nmS2kHDJbgRFQMAVCGAmGc9sUORRK0BwVr0tUIBspjUorpYQ1hldWVSUvuayx4mNyvbu7Cv5rjXHQnYsW2xZ69OuoRSVlyRM_JtMdaIOPMWCjV8F1EDaaUb2VppM0_SctBS73l9emw_oP31tKwNUOWLqP5Y8LqI3zdomdzhXXhdCcKcESpnYYJg3fDoOO1mFvsU4RO-jau_9e-AVet4eq
CitedBy_id crossref_primary_10_1038_nature25023
crossref_primary_10_1021_cb900193e
crossref_primary_10_1074_jbc_M115_661702
crossref_primary_10_1165_rcmb_2019_0255OC
crossref_primary_10_3390_ijms22136897
crossref_primary_10_3389_fnmol_2014_00028
crossref_primary_10_1182_blood_2010_02_269837
crossref_primary_10_1016_j_semcdb_2014_09_024
crossref_primary_10_1186_1476_4598_11_85
crossref_primary_10_1016_j_cellsig_2021_110072
crossref_primary_10_1038_s41598_019_38730_4
crossref_primary_10_1016_j_celrep_2022_110721
crossref_primary_10_1016_j_yexcr_2014_06_022
crossref_primary_10_1111_j_1365_2443_2012_01618_x
crossref_primary_10_1016_j_semcancer_2019_07_003
crossref_primary_10_1038_leu_2011_20
crossref_primary_10_1038_onc_2010_28
crossref_primary_10_1146_annurev_biochem_060614_034335
crossref_primary_10_1534_genetics_113_154005
crossref_primary_10_1093_hmg_ddz042
crossref_primary_10_1016_j_celrep_2014_04_048
crossref_primary_10_1534_genetics_111_133363
crossref_primary_10_1158_2159_8290_CD_13_0353
crossref_primary_10_1152_ajpcell_00081_2017
crossref_primary_10_3109_10409238_2011_618113
crossref_primary_10_1016_j_chom_2018_04_006
crossref_primary_10_1096_fj_201901341R
crossref_primary_10_1111_j_1365_2443_2010_01482_x
crossref_primary_10_1186_s13059_014_0433_z
crossref_primary_10_1371_journal_pone_0109410
crossref_primary_10_18632_oncotarget_10805
crossref_primary_10_1074_jbc_RA120_014994
crossref_primary_10_1093_jb_mvt047
crossref_primary_10_1074_jbc_M115_694331
crossref_primary_10_1038_nature12122
crossref_primary_10_1042_CS20150149
crossref_primary_10_1002_pros_23425
crossref_primary_10_1074_jbc_M112_399485
crossref_primary_10_1371_journal_pone_0037366
crossref_primary_10_1111_cas_12828
crossref_primary_10_1128_MCB_00075_17
crossref_primary_10_1186_s12881_015_0240_8
crossref_primary_10_1038_s41388_019_0787_5
crossref_primary_10_1038_s41379_021_00896_6
crossref_primary_10_3390_genes12111746
crossref_primary_10_1007_s00428_020_02900_x
crossref_primary_10_1038_s41598_019_48172_7
crossref_primary_10_1074_jbc_M111_246397
crossref_primary_10_1038_s41467_022_33669_z
crossref_primary_10_1016_j_isci_2018_06_006
crossref_primary_10_18632_oncotarget_1548
crossref_primary_10_1007_s40259_014_0090_5
crossref_primary_10_1158_1078_0432_CCR_10_1102
crossref_primary_10_1002_ana_24444
crossref_primary_10_1371_journal_pone_0040817
Cites_doi 10.1093/genetics/122.1.19
10.1016/j.molcel.2006.03.029
10.1016/S0076-6879(02)50957-5
10.1073/pnas.0608510104
10.1038/sj.onc.1207542
10.1016/S0092-8674(02)00833-4
10.1073/pnas.93.24.13780
10.1101/gad.1461206
10.1534/genetics.105.051979
10.1016/S1097-2765(03)00114-X
10.1093/emboj/19.21.5720
10.1038/ng1362
10.1016/S1097-2765(02)00636-6
10.1074/jbc.M603566200
10.1111/j.1356-9597.2004.00727.x
10.1016/0092-8674(93)90144-F
10.1038/ncb839
10.1073/pnas.0308061100
10.1074/jbc.M306337200
10.1128/MMBR.69.1.79-100.2005
10.1016/j.dnarep.2004.04.002
10.1016/j.biocel.2004.02.002
10.1101/gad.1199104
10.1074/jbc.M603107200
10.4161/auto.4600
10.1038/ncb847
10.1073/pnas.98.1.136
10.1074/jbc.272.42.26457
10.1016/S0092-8674(02)00741-9
10.1016/j.cell.2006.01.016
10.1126/science.1147379
10.1128/MCB.22.17.6247-6260.2002
10.1126/science.1106148
10.1083/jcb.150.6.1507
10.1016/j.cub.2006.08.001
10.1074/jbc.M606087200
10.1016/j.cub.2004.06.054
10.1016/S0092-8674(02)00808-5
10.1074/jbc.M505553200
10.1101/gad.10.3.279
10.1074/jbc.M003633200
10.1016/j.molcel.2007.06.028
10.1101/gad.995802
10.1038/ncb1183
10.1016/j.molmed.2005.06.007
10.1016/j.cell.2006.08.033
10.1101/gad.1314605
10.1038/345544a0
ContentType Journal Article
Copyright 2008 © 2008 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
Copyright_xml – notice: 2008 © 2008 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
DBID 6I.
AAFTH
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1074/jbc.M801546200
DatabaseName ScienceDirect Open Access Titles
Elsevier:ScienceDirect:Open Access
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE


Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Chemistry
EISSN 1083-351X
EndPage 31870
ExternalDocumentID 10_1074_jbc_M801546200
18812319
283_46_31861
S0021925820569317
Genre Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: Welfide Medicinal Research Foundation
– fundername: Naito Foundation
– fundername: Japan Society for the Promotion of Science
– fundername: Mochida Memorial Foundation for Medical and Pharmaceutical Research
GroupedDBID ---
-DZ
-ET
-~X
.55
0SF
186
18M
29J
2WC
34G
39C
4.4
53G
5BI
5GY
5RE
5VS
6I.
79B
85S
AAEDW
AAFTH
AAFWJ
AARDX
AAXUO
ABDNZ
ABOCM
ABPPZ
ABRJW
ACGFO
ACNCT
ADBBV
ADIYS
ADNWM
AENEX
AEXQZ
AFFNX
AFMIJ
AFOSN
AFPKN
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
AOIJS
BAWUL
BTFSW
C1A
CJ0
CS3
DIK
DU5
E3Z
EBS
EJD
F20
F5P
FDB
FRP
GROUPED_DOAJ
GX1
HH5
HYE
IH2
KQ8
L7B
MVM
N9A
OHT
OK1
P-O
P0W
P2P
R.V
RHF
RHI
RNS
ROL
RPM
SJN
TBC
TN5
TR2
UHB
UKR
UPT
UQL
VQA
W8F
WH7
WOQ
X7M
XFK
XSW
Y6R
YQT
YSK
YWH
YZZ
ZA5
ZE2
~02
~KM
-
02
55
AAWZA
ABFLS
ABPTK
ABUFD
ABZEH
ADACO
ADCOW
AEILP
AIZTS
DL
DZ
ET
FH7
H13
KM
LI
LI0
MYA
O0-
X
XHC
0R~
AALRI
ADVLN
AITUG
AKRWK
CGR
CUY
CVF
ECM
EIF
NPM
.GJ
3O-
41~
6TJ
AAYJJ
AAYOK
AAYXX
ABFSI
ABTAH
ACSFO
ACYGS
AI.
CITATION
E.L
FA8
J5H
NHB
QZG
VH1
WHG
XJT
YYP
ZGI
ZY4
7X8
ID FETCH-LOGICAL-c477t-c9ab6a418a66b7ab33cf42a48602e83d5e8a7f77b8b33886cbb39c8953537de93
ISSN 0021-9258
IngestDate Fri Oct 25 05:46:28 EDT 2024
Fri Dec 06 01:40:29 EST 2024
Sat Sep 28 08:40:20 EDT 2024
Tue Jan 05 14:52:23 EST 2021
Fri Feb 23 02:45:00 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 46
Language English
License This is an open access article under the CC BY license.
http://creativecommons.org/licenses/by/4.0
https://www.elsevier.com/tdm/userlicense/1.0
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c477t-c9ab6a418a66b7ab33cf42a48602e83d5e8a7f77b8b33886cbb39c8953537de93
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://dx.doi.org/10.1074/jbc.M801546200
PMID 18812319
PQID 69775395
PQPubID 23479
PageCount 10
ParticipantIDs proquest_miscellaneous_69775395
crossref_primary_10_1074_jbc_M801546200
pubmed_primary_18812319
highwire_biochem_283_46_31861
elsevier_sciencedirect_doi_10_1074_jbc_M801546200
ProviderPackageCode RHF
RHI
PublicationCentury 2000
PublicationDate 2008-11-14
PublicationDateYYYYMMDD 2008-11-14
PublicationDate_xml – month: 11
  year: 2008
  text: 2008-11-14
  day: 14
PublicationDecade 2000
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle The Journal of biological chemistry
PublicationTitleAlternate J Biol Chem
PublicationYear 2008
Publisher Elsevier Inc
American Society for Biochemistry and Molecular Biology
Publisher_xml – name: Elsevier Inc
– name: American Society for Biochemistry and Molecular Biology
References Sarbassov, Ali, Sengupta, Sheen, Hsu, Bagley, Markhard, Sabatini (bib18) 2006; 22
Kanazawa, Taneike, Akaishi, Yoshizawa, Furuya, Fujimura, Kadowaki (bib33) 2004; 279
Yang, Inoki, Ikenoue, Guan (bib10) 2006; 20
Ravikumar, Vacher, Berger, Davies, Luo, Oroz, Scaravilli, Easton, Duden, O'Kane, Rubinsztein (bib40) 2004; 36
Abraham (bib3) 2004; 3
Meijer, Codogno (bib31) 2004; 36
Jacinto, Loewith, Schmidt, Lin, Rüegg, Hall, Hall (bib9) 2004; 6
Schmidt, Kunz, Hall (bib22) 1996; 93
Sikorski, Hieter (bib26) 1989; 122
Urano, Sato, Matsuo, Otsubo, Yamamoto, Tamanoi (bib30) 2007; 104
Mizushima, Yoshimori (bib34) 2007; 3
Kim, Sarbassov, Ali, Latek, Guntur, Erdjument-Bromage, Tempst, Sabatini (bib7) 2003; 11
Chen, Ma, Li, Eliseenkova, Xu, Neubert, Miller, Mohammadi (bib37) 2007; 27
Hara, Maruki, Long, Yoshino, Oshiro, Hidayat, Tokunaga, Avruch, Yonezawa (bib5) 2002; 110
Wullschleger, Loewith, Oppliger, Hall (bib14) 2005; 280
Zhang, Billington, Pan, Neufeld (bib16) 2006; 172
Hara, Yonezawa, Kozlowski, Sugimoto, Andrabi, Weng, Kasuga, Nishimoto, Avruch (bib27) 1997; 272
Inoki, Li, Zhu, Wu, Guan (bib43) 2002; 4
Gietz, Woods (bib28) 2002; 350
Wang, Rhodes, Lawrence (bib15) 2006; 281
Loewith, Jacinto, Wullschleger, Lorberg, Crespo, Bonenfant, Oppliger, Jenoe, Hall (bib4) 2002; 10
Kim, Sarbassov, Ali, King, Latek, Erdjument-Bromage, Tempst, Sabatini (bib6) 2002; 110
Aoki, Blazek, Vogt (bib41) 2001; 98
Sarbassov, Guertin, Ali, Sabatini (bib21) 2005; 307
Shaw, Kosmatka, Bardeesy, Hurley, Witters, DePinho, Cantley (bib45) 2004; 101
Kabeya, Mizushima, Ueno, Yamamoto, Kirisako, Noda, Kominami, Ohsumi, Yoshimori (bib35) 2000; 19
Sarbassov, Ali, Kim, Guertin, Latek, Erdjument-Bromage, Tempst, Sabatini (bib8) 2004; 14
Scheid, Marignani, Woodgett (bib48) 2002; 22
Frias, Thoreen, Jaffe, Schroder, Sculley, Carr, Sabatini (bib12) 2006; 16
Bai, Ma, Liu, Shen, Wang, Liu, Jiang (bib38) 2007; 318
Fingar, Salama, Tsou, Harlow, Blenis (bib39) 2002; 16
Lazaris-Karatzas, Montine, Sonenberg (bib42) 1990; 345
Gao, Zhang, Arrazola, Hino, Kobayashi, Yeung, Ru, Pan (bib44) 2002; 4
Inoki, Ouyang, Li, Guan (bib1) 2005; 69
Manning, Logsdon, Lipovsky, Abbott, Kwiatkowski, Cantley (bib47) 2005; 19
Fingar, Blenis (bib19) 2004; 23
Kunz, Henriquez, Schneider, Deuter-Reinhard, Movva, Hall (bib25) 1993; 73
Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi (bib20) 2000; 150
Oshiro, Yoshino, Hidayat, Tokunaga, Hara, Eguchi, Avruch, Yonezawa (bib17) 2004; 9
Corradetti, Inoki, Bardeesy, DePinho, Guan (bib46) 2004; 18
Alarcon, Cardenas, Heitman (bib24) 1996; 10
Reinke, Chen, Aronova, Powers (bib29) 2006; 281
Mordier, Deval, Béchet, Tassa, Ferrara (bib32) 2000; 275
Huse, Kuriyan (bib36) 2002; 109
Guertin, Sabatini (bib23) 2005; 11
Jacinto, Facchinetti, Liu, Soto, Wei, Jung, Huang, Qin, Su (bib11) 2006; 127
Takahara, Hara, Yonezawa, Sorimachi, Maeda (bib13) 2006; 281
Wullschleger, Loewith, Hall (bib2) 2006; 124
Jacinto (10.1074/jbc.M801546200_bib9) 2004; 6
Reinke (10.1074/jbc.M801546200_bib29) 2006; 281
Wullschleger (10.1074/jbc.M801546200_bib14) 2005; 280
Kabeya (10.1074/jbc.M801546200_bib35) 2000; 19
Schmidt (10.1074/jbc.M801546200_bib22) 1996; 93
Sarbassov (10.1074/jbc.M801546200_bib21) 2005; 307
Kanazawa (10.1074/jbc.M801546200_bib33) 2004; 279
Chen (10.1074/jbc.M801546200_bib37) 2007; 27
Takahara (10.1074/jbc.M801546200_bib13) 2006; 281
Kamada (10.1074/jbc.M801546200_bib20) 2000; 150
Aoki (10.1074/jbc.M801546200_bib41) 2001; 98
Wullschleger (10.1074/jbc.M801546200_bib2) 2006; 124
Mizushima (10.1074/jbc.M801546200_bib34) 2007; 3
Corradetti (10.1074/jbc.M801546200_bib46) 2004; 18
Sikorski (10.1074/jbc.M801546200_bib26) 1989; 122
Sarbassov (10.1074/jbc.M801546200_bib8) 2004; 14
Oshiro (10.1074/jbc.M801546200_bib17) 2004; 9
Kunz (10.1074/jbc.M801546200_bib25) 1993; 73
Inoki (10.1074/jbc.M801546200_bib43) 2002; 4
Fingar (10.1074/jbc.M801546200_bib39) 2002; 16
Ravikumar (10.1074/jbc.M801546200_bib40) 2004; 36
Zhang (10.1074/jbc.M801546200_bib16) 2006; 172
Mordier (10.1074/jbc.M801546200_bib32) 2000; 275
Inoki (10.1074/jbc.M801546200_bib1) 2005; 69
Yang (10.1074/jbc.M801546200_bib10) 2006; 20
Abraham (10.1074/jbc.M801546200_bib3) 2004; 3
Kim (10.1074/jbc.M801546200_bib6) 2002; 110
Jacinto (10.1074/jbc.M801546200_bib11) 2006; 127
Kim (10.1074/jbc.M801546200_bib7) 2003; 11
Bai (10.1074/jbc.M801546200_bib38) 2007; 318
Frias (10.1074/jbc.M801546200_bib12) 2006; 16
Shaw (10.1074/jbc.M801546200_bib45) 2004; 101
Hara (10.1074/jbc.M801546200_bib5) 2002; 110
Fingar (10.1074/jbc.M801546200_bib19) 2004; 23
Hara (10.1074/jbc.M801546200_bib27) 1997; 272
Lazaris-Karatzas (10.1074/jbc.M801546200_bib42) 1990; 345
Manning (10.1074/jbc.M801546200_bib47) 2005; 19
Sarbassov (10.1074/jbc.M801546200_bib18) 2006; 22
Gietz (10.1074/jbc.M801546200_bib28) 2002; 350
Meijer (10.1074/jbc.M801546200_bib31) 2004; 36
Huse (10.1074/jbc.M801546200_bib36) 2002; 109
Wang (10.1074/jbc.M801546200_bib15) 2006; 281
Alarcon (10.1074/jbc.M801546200_bib24) 1996; 10
Guertin (10.1074/jbc.M801546200_bib23) 2005; 11
Gao (10.1074/jbc.M801546200_bib44) 2002; 4
Urano (10.1074/jbc.M801546200_bib30) 2007; 104
Loewith (10.1074/jbc.M801546200_bib4) 2002; 10
Scheid (10.1074/jbc.M801546200_bib48) 2002; 22
References_xml – volume: 14
  start-page: 1296
  year: 2004
  end-page: 1302
  ident: bib8
  publication-title: Curr. Biol.
  contributor:
    fullname: Sabatini
– volume: 16
  start-page: 1865
  year: 2006
  end-page: 1870
  ident: bib12
  publication-title: Curr. Biol.
  contributor:
    fullname: Sabatini
– volume: 22
  start-page: 6247
  year: 2002
  end-page: 6260
  ident: bib48
  publication-title: Mol. Cell. Biol.
  contributor:
    fullname: Woodgett
– volume: 110
  start-page: 177
  year: 2002
  end-page: 189
  ident: bib5
  publication-title: Cell
  contributor:
    fullname: Yonezawa
– volume: 272
  start-page: 26457
  year: 1997
  end-page: 26463
  ident: bib27
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Avruch
– volume: 11
  start-page: 895
  year: 2003
  end-page: 904
  ident: bib7
  publication-title: Mol. Cell
  contributor:
    fullname: Sabatini
– volume: 307
  start-page: 1098
  year: 2005
  end-page: 1101
  ident: bib21
  publication-title: Science
  contributor:
    fullname: Sabatini
– volume: 122
  start-page: 19
  year: 1989
  end-page: 27
  ident: bib26
  publication-title: Genetics
  contributor:
    fullname: Hieter
– volume: 3
  start-page: 883
  year: 2004
  end-page: 887
  ident: bib3
  publication-title: DNA Repair (Amst)
  contributor:
    fullname: Abraham
– volume: 22
  start-page: 159
  year: 2006
  end-page: 168
  ident: bib18
  publication-title: Mol. Cell
  contributor:
    fullname: Sabatini
– volume: 98
  start-page: 136
  year: 2001
  end-page: 141
  ident: bib41
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  contributor:
    fullname: Vogt
– volume: 281
  start-page: 28605
  year: 2006
  end-page: 28614
  ident: bib13
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Maeda
– volume: 279
  start-page: 8452
  year: 2004
  end-page: 8459
  ident: bib33
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Kadowaki
– volume: 19
  start-page: 5720
  year: 2000
  end-page: 5728
  ident: bib35
  publication-title: EMBO J.
  contributor:
    fullname: Yoshimori
– volume: 9
  start-page: 359
  year: 2004
  end-page: 366
  ident: bib17
  publication-title: Genes Cells
  contributor:
    fullname: Yonezawa
– volume: 27
  start-page: 717
  year: 2007
  end-page: 730
  ident: bib37
  publication-title: Mol. Cell
  contributor:
    fullname: Mohammadi
– volume: 16
  start-page: 1472
  year: 2002
  end-page: 1487
  ident: bib39
  publication-title: Genes Dev.
  contributor:
    fullname: Blenis
– volume: 19
  start-page: 1773
  year: 2005
  end-page: 1778
  ident: bib47
  publication-title: Genes Dev.
  contributor:
    fullname: Cantley
– volume: 345
  start-page: 544
  year: 1990
  end-page: 547
  ident: bib42
  publication-title: Nature
  contributor:
    fullname: Sonenberg
– volume: 4
  start-page: 648
  year: 2002
  end-page: 657
  ident: bib43
  publication-title: Nat. Cell Biol.
  contributor:
    fullname: Guan
– volume: 101
  start-page: 3329
  year: 2004
  end-page: 3335
  ident: bib45
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  contributor:
    fullname: Cantley
– volume: 3
  start-page: 542
  year: 2007
  end-page: 545
  ident: bib34
  publication-title: Autophagy
  contributor:
    fullname: Yoshimori
– volume: 10
  start-page: 279
  year: 1996
  end-page: 288
  ident: bib24
  publication-title: Genes Dev.
  contributor:
    fullname: Heitman
– volume: 10
  start-page: 457
  year: 2002
  end-page: 468
  ident: bib4
  publication-title: Mol. Cell
  contributor:
    fullname: Hall
– volume: 36
  start-page: 2445
  year: 2004
  end-page: 2462
  ident: bib31
  publication-title: Int. J. Biochem. Cell Biol.
  contributor:
    fullname: Codogno
– volume: 172
  start-page: 355
  year: 2006
  end-page: 362
  ident: bib16
  publication-title: Genetics
  contributor:
    fullname: Neufeld
– volume: 350
  start-page: 87
  year: 2002
  end-page: 96
  ident: bib28
  publication-title: Methods Enzymol.
  contributor:
    fullname: Woods
– volume: 281
  start-page: 24293
  year: 2006
  end-page: 24303
  ident: bib15
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Lawrence
– volume: 20
  start-page: 2820
  year: 2006
  end-page: 2832
  ident: bib10
  publication-title: Genes Dev.
  contributor:
    fullname: Guan
– volume: 124
  start-page: 471
  year: 2006
  end-page: 484
  ident: bib2
  publication-title: Cell
  contributor:
    fullname: Hall
– volume: 93
  start-page: 13780
  year: 1996
  end-page: 13785
  ident: bib22
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  contributor:
    fullname: Hall
– volume: 150
  start-page: 1507
  year: 2000
  end-page: 1513
  ident: bib20
  publication-title: J. Cell Biol.
  contributor:
    fullname: Ohsumi
– volume: 69
  start-page: 79
  year: 2005
  end-page: 100
  ident: bib1
  publication-title: Microbiol. Mol. Biol. Rev.
  contributor:
    fullname: Guan
– volume: 104
  start-page: 3514
  year: 2007
  end-page: 3519
  ident: bib30
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  contributor:
    fullname: Tamanoi
– volume: 23
  start-page: 3151
  year: 2004
  end-page: 3171
  ident: bib19
  publication-title: Oncogene
  contributor:
    fullname: Blenis
– volume: 318
  start-page: 977
  year: 2007
  end-page: 980
  ident: bib38
  publication-title: Science
  contributor:
    fullname: Jiang
– volume: 4
  start-page: 699
  year: 2002
  end-page: 704
  ident: bib44
  publication-title: Nat. Cell Biol.
  contributor:
    fullname: Pan
– volume: 73
  start-page: 585
  year: 1993
  end-page: 596
  ident: bib25
  publication-title: Cell
  contributor:
    fullname: Hall
– volume: 11
  start-page: 353
  year: 2005
  end-page: 361
  ident: bib23
  publication-title: Trends Mol. Med.
  contributor:
    fullname: Sabatini
– volume: 109
  start-page: 275
  year: 2002
  end-page: 282
  ident: bib36
  publication-title: Cell
  contributor:
    fullname: Kuriyan
– volume: 6
  start-page: 1122
  year: 2004
  end-page: 1128
  ident: bib9
  publication-title: Nat. Cell Biol.
  contributor:
    fullname: Hall
– volume: 127
  start-page: 125
  year: 2006
  end-page: 137
  ident: bib11
  publication-title: Cell
  contributor:
    fullname: Su
– volume: 280
  start-page: 30697
  year: 2005
  end-page: 30704
  ident: bib14
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Hall
– volume: 18
  start-page: 1533
  year: 2004
  end-page: 1538
  ident: bib46
  publication-title: Genes Dev.
  contributor:
    fullname: Guan
– volume: 36
  start-page: 585
  year: 2004
  end-page: 595
  ident: bib40
  publication-title: Nat. Genet.
  contributor:
    fullname: Rubinsztein
– volume: 110
  start-page: 163
  year: 2002
  end-page: 175
  ident: bib6
  publication-title: Cell
  contributor:
    fullname: Sabatini
– volume: 281
  start-page: 31616
  year: 2006
  end-page: 31626
  ident: bib29
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Powers
– volume: 275
  start-page: 29900
  year: 2000
  end-page: 29906
  ident: bib32
  publication-title: J. Biol. Chem.
  contributor:
    fullname: Ferrara
– volume: 122
  start-page: 19
  year: 1989
  ident: 10.1074/jbc.M801546200_bib26
  publication-title: Genetics
  doi: 10.1093/genetics/122.1.19
  contributor:
    fullname: Sikorski
– volume: 22
  start-page: 159
  year: 2006
  ident: 10.1074/jbc.M801546200_bib18
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2006.03.029
  contributor:
    fullname: Sarbassov
– volume: 350
  start-page: 87
  year: 2002
  ident: 10.1074/jbc.M801546200_bib28
  publication-title: Methods Enzymol.
  doi: 10.1016/S0076-6879(02)50957-5
  contributor:
    fullname: Gietz
– volume: 104
  start-page: 3514
  year: 2007
  ident: 10.1074/jbc.M801546200_bib30
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  doi: 10.1073/pnas.0608510104
  contributor:
    fullname: Urano
– volume: 23
  start-page: 3151
  year: 2004
  ident: 10.1074/jbc.M801546200_bib19
  publication-title: Oncogene
  doi: 10.1038/sj.onc.1207542
  contributor:
    fullname: Fingar
– volume: 110
  start-page: 177
  year: 2002
  ident: 10.1074/jbc.M801546200_bib5
  publication-title: Cell
  doi: 10.1016/S0092-8674(02)00833-4
  contributor:
    fullname: Hara
– volume: 93
  start-page: 13780
  year: 1996
  ident: 10.1074/jbc.M801546200_bib22
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  doi: 10.1073/pnas.93.24.13780
  contributor:
    fullname: Schmidt
– volume: 20
  start-page: 2820
  year: 2006
  ident: 10.1074/jbc.M801546200_bib10
  publication-title: Genes Dev.
  doi: 10.1101/gad.1461206
  contributor:
    fullname: Yang
– volume: 172
  start-page: 355
  year: 2006
  ident: 10.1074/jbc.M801546200_bib16
  publication-title: Genetics
  doi: 10.1534/genetics.105.051979
  contributor:
    fullname: Zhang
– volume: 11
  start-page: 895
  year: 2003
  ident: 10.1074/jbc.M801546200_bib7
  publication-title: Mol. Cell
  doi: 10.1016/S1097-2765(03)00114-X
  contributor:
    fullname: Kim
– volume: 19
  start-page: 5720
  year: 2000
  ident: 10.1074/jbc.M801546200_bib35
  publication-title: EMBO J.
  doi: 10.1093/emboj/19.21.5720
  contributor:
    fullname: Kabeya
– volume: 36
  start-page: 585
  year: 2004
  ident: 10.1074/jbc.M801546200_bib40
  publication-title: Nat. Genet.
  doi: 10.1038/ng1362
  contributor:
    fullname: Ravikumar
– volume: 10
  start-page: 457
  year: 2002
  ident: 10.1074/jbc.M801546200_bib4
  publication-title: Mol. Cell
  doi: 10.1016/S1097-2765(02)00636-6
  contributor:
    fullname: Loewith
– volume: 281
  start-page: 24293
  year: 2006
  ident: 10.1074/jbc.M801546200_bib15
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M603566200
  contributor:
    fullname: Wang
– volume: 9
  start-page: 359
  year: 2004
  ident: 10.1074/jbc.M801546200_bib17
  publication-title: Genes Cells
  doi: 10.1111/j.1356-9597.2004.00727.x
  contributor:
    fullname: Oshiro
– volume: 73
  start-page: 585
  year: 1993
  ident: 10.1074/jbc.M801546200_bib25
  publication-title: Cell
  doi: 10.1016/0092-8674(93)90144-F
  contributor:
    fullname: Kunz
– volume: 4
  start-page: 648
  year: 2002
  ident: 10.1074/jbc.M801546200_bib43
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb839
  contributor:
    fullname: Inoki
– volume: 101
  start-page: 3329
  year: 2004
  ident: 10.1074/jbc.M801546200_bib45
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  doi: 10.1073/pnas.0308061100
  contributor:
    fullname: Shaw
– volume: 279
  start-page: 8452
  year: 2004
  ident: 10.1074/jbc.M801546200_bib33
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M306337200
  contributor:
    fullname: Kanazawa
– volume: 69
  start-page: 79
  year: 2005
  ident: 10.1074/jbc.M801546200_bib1
  publication-title: Microbiol. Mol. Biol. Rev.
  doi: 10.1128/MMBR.69.1.79-100.2005
  contributor:
    fullname: Inoki
– volume: 3
  start-page: 883
  year: 2004
  ident: 10.1074/jbc.M801546200_bib3
  publication-title: DNA Repair (Amst)
  doi: 10.1016/j.dnarep.2004.04.002
  contributor:
    fullname: Abraham
– volume: 36
  start-page: 2445
  year: 2004
  ident: 10.1074/jbc.M801546200_bib31
  publication-title: Int. J. Biochem. Cell Biol.
  doi: 10.1016/j.biocel.2004.02.002
  contributor:
    fullname: Meijer
– volume: 18
  start-page: 1533
  year: 2004
  ident: 10.1074/jbc.M801546200_bib46
  publication-title: Genes Dev.
  doi: 10.1101/gad.1199104
  contributor:
    fullname: Corradetti
– volume: 281
  start-page: 31616
  year: 2006
  ident: 10.1074/jbc.M801546200_bib29
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M603107200
  contributor:
    fullname: Reinke
– volume: 3
  start-page: 542
  year: 2007
  ident: 10.1074/jbc.M801546200_bib34
  publication-title: Autophagy
  doi: 10.4161/auto.4600
  contributor:
    fullname: Mizushima
– volume: 4
  start-page: 699
  year: 2002
  ident: 10.1074/jbc.M801546200_bib44
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb847
  contributor:
    fullname: Gao
– volume: 98
  start-page: 136
  year: 2001
  ident: 10.1074/jbc.M801546200_bib41
  publication-title: Proc. Natl. Acad. Sci. U. S. A
  doi: 10.1073/pnas.98.1.136
  contributor:
    fullname: Aoki
– volume: 272
  start-page: 26457
  year: 1997
  ident: 10.1074/jbc.M801546200_bib27
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.272.42.26457
  contributor:
    fullname: Hara
– volume: 109
  start-page: 275
  year: 2002
  ident: 10.1074/jbc.M801546200_bib36
  publication-title: Cell
  doi: 10.1016/S0092-8674(02)00741-9
  contributor:
    fullname: Huse
– volume: 124
  start-page: 471
  year: 2006
  ident: 10.1074/jbc.M801546200_bib2
  publication-title: Cell
  doi: 10.1016/j.cell.2006.01.016
  contributor:
    fullname: Wullschleger
– volume: 318
  start-page: 977
  year: 2007
  ident: 10.1074/jbc.M801546200_bib38
  publication-title: Science
  doi: 10.1126/science.1147379
  contributor:
    fullname: Bai
– volume: 22
  start-page: 6247
  year: 2002
  ident: 10.1074/jbc.M801546200_bib48
  publication-title: Mol. Cell. Biol.
  doi: 10.1128/MCB.22.17.6247-6260.2002
  contributor:
    fullname: Scheid
– volume: 307
  start-page: 1098
  year: 2005
  ident: 10.1074/jbc.M801546200_bib21
  publication-title: Science
  doi: 10.1126/science.1106148
  contributor:
    fullname: Sarbassov
– volume: 150
  start-page: 1507
  year: 2000
  ident: 10.1074/jbc.M801546200_bib20
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.150.6.1507
  contributor:
    fullname: Kamada
– volume: 16
  start-page: 1865
  year: 2006
  ident: 10.1074/jbc.M801546200_bib12
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2006.08.001
  contributor:
    fullname: Frias
– volume: 281
  start-page: 28605
  year: 2006
  ident: 10.1074/jbc.M801546200_bib13
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M606087200
  contributor:
    fullname: Takahara
– volume: 14
  start-page: 1296
  year: 2004
  ident: 10.1074/jbc.M801546200_bib8
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2004.06.054
  contributor:
    fullname: Sarbassov
– volume: 110
  start-page: 163
  year: 2002
  ident: 10.1074/jbc.M801546200_bib6
  publication-title: Cell
  doi: 10.1016/S0092-8674(02)00808-5
  contributor:
    fullname: Kim
– volume: 280
  start-page: 30697
  year: 2005
  ident: 10.1074/jbc.M801546200_bib14
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M505553200
  contributor:
    fullname: Wullschleger
– volume: 10
  start-page: 279
  year: 1996
  ident: 10.1074/jbc.M801546200_bib24
  publication-title: Genes Dev.
  doi: 10.1101/gad.10.3.279
  contributor:
    fullname: Alarcon
– volume: 275
  start-page: 29900
  year: 2000
  ident: 10.1074/jbc.M801546200_bib32
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M003633200
  contributor:
    fullname: Mordier
– volume: 27
  start-page: 717
  year: 2007
  ident: 10.1074/jbc.M801546200_bib37
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2007.06.028
  contributor:
    fullname: Chen
– volume: 16
  start-page: 1472
  year: 2002
  ident: 10.1074/jbc.M801546200_bib39
  publication-title: Genes Dev.
  doi: 10.1101/gad.995802
  contributor:
    fullname: Fingar
– volume: 6
  start-page: 1122
  year: 2004
  ident: 10.1074/jbc.M801546200_bib9
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb1183
  contributor:
    fullname: Jacinto
– volume: 11
  start-page: 353
  year: 2005
  ident: 10.1074/jbc.M801546200_bib23
  publication-title: Trends Mol. Med.
  doi: 10.1016/j.molmed.2005.06.007
  contributor:
    fullname: Guertin
– volume: 127
  start-page: 125
  year: 2006
  ident: 10.1074/jbc.M801546200_bib11
  publication-title: Cell
  doi: 10.1016/j.cell.2006.08.033
  contributor:
    fullname: Jacinto
– volume: 19
  start-page: 1773
  year: 2005
  ident: 10.1074/jbc.M801546200_bib47
  publication-title: Genes Dev.
  doi: 10.1101/gad.1314605
  contributor:
    fullname: Manning
– volume: 345
  start-page: 544
  year: 1990
  ident: 10.1074/jbc.M801546200_bib42
  publication-title: Nature
  doi: 10.1038/345544a0
  contributor:
    fullname: Lazaris-Karatzas
SSID ssj0000491
Score 2.2419963
Snippet The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as...
The mammalian target of rapamycin (mTOR) is a Ser/Thr kinase that plays essential roles in the regulation of a wide array of growth-related processes such as...
SourceID proquest
crossref
pubmed
highwire
elsevier
SourceType Aggregation Database
Index Database
Publisher
StartPage 31861
SubjectTerms Animals
Autophagy
Cell Line
Cell Size
Enzyme Activation
Gene Expression Regulation, Enzymologic
Humans
Mechanistic Target of Rapamycin Complex 1
Mice
Multiprotein Complexes
Mutation - genetics
Protein Kinases - genetics
Protein Kinases - metabolism
Proteins
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction
TOR Serine-Threonine Kinases
Transcription Factors - metabolism
Title Isolation of Hyperactive Mutants of Mammalian Target of Rapamycin
URI https://dx.doi.org/10.1074/jbc.M801546200
http://www.jbc.org/content/283/46/31861.abstract
https://www.ncbi.nlm.nih.gov/pubmed/18812319
https://search.proquest.com/docview/69775395
Volume 283
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Nb9MwFLfKOMAFwcZHgUEOCE4pJHb8cawmUAEVCamTysmyHUcrUxLUJYftxJ_Os52PDqgEu0StrSau3y_2z_Z7v4fQK5WmeZpYFlssdExEwWOdUBEDOS-osDklPhZm-YUuTsmndbaeTH7ueC21jZ6Zq7_GldzEqlAGdnVRsv9h2eGmUACfwb5wBQvD9Z9s_BFuP1C-BawofciTSyfUNqN_S1mGvYyVd_r26iMwQ5aXphPd_j4CZoeeBnWmoB_SJ4UbdmTPwj7ot3pjzjbbelz-nysnAO1BYLdtpepxhGsUjBeqDGR1cz7ULFVz0V6pkD57VZd1V9VvRXAXk5eMW5FDjMw1F87gBJIGgfaZDcMsED8XQ7DeHYfTkNGmAxzZHVZh4AmS7d0cDd9DupE_JgBgRG4C0Ga25I4e0iCE-ruotjujTlyjUiCAAjjULXTbSSm67Aufv45687B-CjkXu__Qy34y8vb6M_bRmkF1ev8KxjOZ1X10r7NxNA94eoAmtjpER_NKNXV5Gb2OvFOwP205RHdOetsfofkAt6guoh24RR3cXPEAtyjAzZUNcHuITj-8X50s4i4DR2wIY01shNJUkYQrSjVTGmNTkFT5xGWW4zyzXLGCMc2hinNqtMbCcJHhDLPcCvwIHVR1ZZ-giIg8ywl_pzJjSM4SwRPCODEJNsCeCjpFb_oOlD-C0Ir0DhKMSOhqOXb1FCV9_8qOJgb6JwEEe39z3BtCwvvj3hsJeJOESo-tKXrZW0dCr7pDM1XZur2QFBZIGRbZFD0ORhtbx4Ecwwz29AbteYbuji_Qc3TQbFt7DOy20S88AH8BbeuiDQ
link.rule.ids 314,780,784,27924,27925
linkProvider Colorado Alliance of Research Libraries
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Isolation+of+Hyperactive+Mutants+of+Mammalian+Target+of+Rapamycin&rft.jtitle=The+Journal+of+biological+chemistry&rft.au=Ohne%2C+Yoichiro&rft.au=Takahara%2C+Terunao&rft.au=Hatakeyama%2C+Riko&rft.au=Matsuzaki%2C+Tomoko&rft.date=2008-11-14&rft.pub=Elsevier+Inc&rft.issn=0021-9258&rft.eissn=1083-351X&rft.volume=283&rft.issue=46&rft.spage=31861&rft.epage=31870&rft_id=info:doi/10.1074%2Fjbc.M801546200&rft.externalDocID=S0021925820569317
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-9258&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-9258&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-9258&client=summon