Gene Targeting by Linear Duplex DNA Frequently Occurs by Assimilation of a Single Strand that is Subject to Preferential Mismatch Correction

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 94; no. 13; pp. 6851 - 6856
• Main Authors: W.-Y. Leung, Malkova, A., Haber, J. E.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 24.06.1997; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences of the USA
• Subjects: 3-Isopropylmalate Dehydrogenase; Alcohol Oxidoreductases - genetics; Alleles; Annealing; Biological Sciences; Chromosomes; Deoxyribonucleic acid; DNA; DNA - genetics; DNA mismatch repair; DNA, Single-Stranded - genetics; Gene conversion; Gene Targeting; Genes; Genetic mutation; Genetics; Nucleic acid heteroduplexes; Plasmids; Recombination, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics

To study targeted recombination, a single linear 2-kb fragment of LEU2 DNA was liberated from a chromosomal site within the nucleus of Saccharomyces cerevisiae, by expression of the site-specific HO endonuclease. Gene targeting was scored by gene conversion of a chromosomal leu2 mutant allele by the...