Effect of nanoparticle coating on the immunogenicity of plasmid DNA vaccine encoding P. yoelii MSP-1 C-terminal

• Published in: Vaccine Vol. 29; no. 17; pp. 3239 - 3247
• Main Authors: Shuaibu, M.N, Cherif, M.S, Kurosaki, T, Helegbe, G.K, Kikuchi, M, Yanagi, T, Sasaki, H, Hirayama, K
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 12.04.2011
• Subjects: Allergy and Immunology; Animals; Antibodies, Protozoan - blood; blood serum; CD4-positive T-lymphocytes; CD4-Positive T-Lymphocytes - immunology; coatings; Cytokines; Cytokines - secretion; DNA vaccination; Drug Carriers - administration & dosage; Drug Carriers - chemistry; enzyme-linked immunosorbent assay; erythrocytes; Female; IgG subtypes; immune response; Immunoassay; immunoglobulin G; Immunoglobulin G - blood; Injections, Intravenous; interferon-gamma; interleukin-4; Intravenous; intravenous injection; Malaria; Malaria - immunology; Malaria - prevention & control; Malaria Vaccines - administration & dosage; Malaria Vaccines - genetics; Malaria Vaccines - immunology; Merozoite Surface Protein 1 - genetics; Merozoite Surface Protein 1 - immunology; Mice; Mice, Inbred C57BL; Nanoparticle; nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; plasmids; Plasmodium yoelii; Plasmodium yoelii - genetics; Plasmodium yoelii - immunology; polyglutamic acid; recombinant vaccines; splenocytes; Survival Analysis; Th2 Cells - immunology; vaccination; Vaccination - methods; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Western blotting; DNA vaccination; Malaria; Cytokines; Nanoparticle; Intravenous; IgG subtypes

Abstract In order to assess a new strategy for DNA vaccine formulation and delivery, plasmid encoding Plasmodium yoelii MSP-1 C-terminal was formulated with newly designed nanoparticle—an anionic ternary complex of polyethylenimine and γ-polyglutamic acid (pVAX-MSP-1/PEI/γ-PGA), and intravenously ad...