Otoferlin interacts with myosin VI: implications for maintenance of the basolateral synaptic structure of the inner hair cell
Otoferlin has been proposed to be the Ca2+ sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and synaptotagmin-2. In the present study, yeast two-hybrid assays reveal myosin VI as a novel otoferlin binding partner. Co-immunoprecipitation assay an...
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Published in | Human molecular genetics Vol. 18; no. 15; pp. 2779 - 2790 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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Oxford
Oxford University Press
01.08.2009
Oxford Publishing Limited (England) |
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Abstract | Otoferlin has been proposed to be the Ca2+ sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and synaptotagmin-2. In the present study, yeast two-hybrid assays reveal myosin VI as a novel otoferlin binding partner. Co-immunoprecipitation assay and co-expression suggest an interaction of both proteins within the basolateral part of inner hair cells (IHCs). Comparison of otoferlin mutants and myosin VI mutant mice indicates non-complementary and complementary roles of myosin VI and otoferlin for synaptic maturation: (i) IHCs from otoferlin mutant mice exhibited a decoupling of CtBP2/RIBEYE and CaV1.3 and severe reduction of exocytosis. (ii) Myosin VI mutant IHCs failed to transport BK channels to the membrane of the apical cell regions, and the exocytotic Ca2+ efficiency did not mature. (iii) Otoferlin and myosin VI mutant IHCs showed a reduced basolateral synaptic surface area and altered active zone topography. Membrane infoldings in otoferlin mutant IHCs indicated disturbed transport of endocytotic membranes and link the above morphological changes to a complementary role of otoferlin and myosin VI in transport of intracellular compartments to the basolateral IHC membrane. |
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AbstractList | Otoferlin has been proposed to be the Ca2+ sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and synaptotagmin-2. In the present study, yeast two-hybrid assays reveal myosin VI as a novel otoferlin binding partner. Co-immunoprecipitation assay and co-expression suggest an interaction of both proteins within the basolateral part of inner hair cells (IHCs). Comparison of otoferlin mutants and myosin VI mutant mice indicates non-complementary and complementary roles of myosin VI and otoferlin for synaptic maturation: (i) IHCs from otoferlin mutant mice exhibited a decoupling of CtBP2/RIBEYE and CaV1.3 and severe reduction of exocytosis. (ii) Myosin VI mutant IHCs failed to transport BK channels to the membrane of the apical cell regions, and the exocytotic Ca2+ efficiency did not mature. (iii) Otoferlin and myosin VI mutant IHCs showed a reduced basolateral synaptic surface area and altered active zone topography. Membrane infoldings in otoferlin mutant IHCs indicated disturbed transport of endocytotic membranes and link the above morphological changes to a complementary role of otoferlin and myosin VI in transport of intracellular compartments to the basolateral IHC membrane. Otoferlin has been proposed to be the Ca(2+) sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and synaptotagmin-2. In the present study, yeast two-hybrid assays reveal myosin VI as a novel otoferlin binding partner. Co-immunoprecipitation assay and co-expression suggest an interaction of both proteins within the basolateral part of inner hair cells (IHCs). Comparison of otoferlin mutants and myosin VI mutant mice indicates non-complementary and complementary roles of myosin VI and otoferlin for synaptic maturation: (i) IHCs from otoferlin mutant mice exhibited a decoupling of CtBP2/RIBEYE and Ca(V)1.3 and severe reduction of exocytosis. (ii) Myosin VI mutant IHCs failed to transport BK channels to the membrane of the apical cell regions, and the exocytotic Ca(2+) efficiency did not mature. (iii) Otoferlin and myosin VI mutant IHCs showed a reduced basolateral synaptic surface area and altered active zone topography. Membrane infoldings in otoferlin mutant IHCs indicated disturbed transport of endocytotic membranes and link the above morphological changes to a complementary role of otoferlin and myosin VI in transport of intracellular compartments to the basolateral IHC membrane. Otoferlin has been proposed to be the Ca[sup]2+ sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and synaptotagmin-2. In the present study, yeast two-hybrid assays reveal myosin VI as a novel otoferlin binding partner. Co-immunoprecipitation assay and co-expression suggest an interaction of both proteins within the basolateral part of inner hair cells (IHCs). Comparison of otoferlin mutants and myosin VI mutant mice indicates non-complementary and complementary roles of myosin VI and otoferlin for synaptic maturation: (i) IHCs from otoferlin mutant mice exhibited a decoupling of CtBP2/RIBEYE and Ca[sub]V1.3 and severe reduction of exocytosis. (ii) Myosin VI mutant IHCs failed to transport BK channels to the membrane of the apical cell regions, and the exocytotic Ca[sup]2+ efficiency did not mature. (iii) Otoferlin and myosin VI mutant IHCs showed a reduced basolateral synaptic surface area and altered active zone topography. Membrane infoldings in otoferlin mutant IHCs indicated disturbed transport of endocytotic membranes and link the above morphological changes to a complementary role of otoferlin and myosin VI in transport of intracellular compartments to the basolateral IHC membrane. |
Author | Franz, Christoph Kuhn, Stephanie Duncker, Susanne V. Blin, Nikolaus Zimmermann, Ulrike Heidrych, Paulina Hirt, Bernhard Ruth, Peter Engel, Jutta Pusch, Carsten M. Pfister, Markus Marcotti, Walter Knipper, Marlies |
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Cites_doi | 10.1086/303049 10.1016/j.cell.2006.08.040 10.1371/journal.pgen.1000207 10.1016/j.bbrc.2007.11.150 10.1016/j.conb.2008.09.006 10.1034/j.1600-0854.2002.31201.x 10.1113/jphysiol.2004.074740 10.1016/S0955-0674(00)00120-4 10.1006/dbio.1999.9424 10.1038/7693 10.1074/jbc.M803605200 10.1002/(SICI)1096-9861(19990705)409:3<424::AID-CNE7>3.0.CO;2-L 10.1074/jbc.R100052200 10.1242/dev.125.18.3709 10.1523/JNEUROSCI.0785-08.2008 10.1523/JNEUROSCI.23-34-10832.2003 10.1093/hmg/ddn279 10.1086/323156 10.1523/JNEUROSCI.3162-04.2004 10.1523/JNEUROSCI.3965-06.2007 10.1126/science.1126308 10.1523/JNEUROSCI.17-23-09133.1997 10.1093/emboj/cdg055 10.1073/pnas.0402660101 10.1523/JNEUROSCI.10-11-03664.1990 10.1523/JNEUROSCI.5364-06.2007 10.1086/375122 10.1136/jmg.40.1.45 10.1083/jcb.200608126 10.1523/JNEUROSCI.22-05-01648.2002 10.1016/S0092-8674(00)00013-1 10.1073/pnas.97.2.883 10.1038/46835 20026583 10.1101/gr.3826505 10.1111/j.1460-9568.2006.05225.x 10.1152/jn.2000.83.5.3101 10.1006/nbdi.2002.0488 10.1523/JNEUROSCI.3411-05.2005 10.1146/annurev.cellbio.19.110701.161425 10.1523/JNEUROSCI.1124-08.2008 10.1038/ng1295-369 10.1083/jcb.127.2.425 10.1016/S0092-8674(00)80225-1 10.1146/annurev.cellbio.20.012103.094243 20026397 10.1016/j.heares.2007.09.005 10.1111/j.1600-0854.2004.00224.x 10.1523/JNEUROSCI.17-18-06961.1997 10.1091/mbc.E07-09-0902 10.1093/emboj/20.14.3676 10.1093/hmg/6.8.1225 |
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References | Ahmed (15_17591918) 2003; 72 Knipper (54_6131371) 1998; 125 Longo-Guess (18_29904491) 2007; 234 (57_35238522) 2006; 143 Melchionda (16_11261253) 2001; 69 Avraham (14_16878183) 1997; 6 (46_31845245) 2008; 17 (17_19305831) 2005; 15 Buss (19_30691805) 2008; 369 Pyott (26_18492304) 2004; 24 (25_35205173) 2005; 130 Schug (7_23543378) 2006; 24 (40_35018816) 2008; 57 Johnson (9_31553663) 2008; 28 (24_19687269) 2004; 101 Dance (29_18430315) 2004; 5 Spicer (48_10862164) 1999; 409 Hertzano (43_32077442) 2008; 4 Wells (21_10993316) 1999; 401 Platzer (30_10418534) 2000; 102 (6_32604905) 2009; 284 Avraham (11_16094790) 1995; 11 Knirsch (28_28836998) 2007; 27 (38_33072856) 2008; 59 Brandt (31_17933084) 2003; 23 Buss (22_18464596) 2004; 20 Brandt (35_21145172) 2005; 25 Varga (3_17468585) 2003; 40 (20_27987290) 2007; 177 Yasunaga (4_10430050) 2000; 67 Knipper (55_10310767) 2000; 83 Naraghi (37_5720563) 1997; 17 Griesinger (51_17060033) 2002; 22 Nakamura (47_16780040) 1997; 89 Roux (5_22876918) 2006; 127 Roberts (32_9213711) 1990; 10 (49_30494199) 2008; 19 (27_18679199) 2005; 563 Gras (50_17130275) 2002; 22 (41_21896927) 2006; 312 (42_33072860) 2007; 53 Buss (23_17350869) 2002; 3 Meinrenken (36_16945390) 2002; 22 Goodyear (45_32029927) 2008; 28 Muth (53_17867623) 2003; 19 Glowatzki (10_32061758) 2008; 18 Moser (34_6439082) 2000; 97 Buss (56_11229865) 2001; 20 Martinez-Dunst (33_5800212) 1997; 17 Hasson (13_15627607) 1994; 127 (39_19552694) 2002; 277 Yasunaga (1_10774599) 1999; 21 Self (12_11002493) 1999; 214 Mirghomizadeh (2_17156732) 2002; 10 Mostov (52_10373657) 2000; 12 Brandt (8_27936203) 2007; 27 Warner (44_17482705) 2003; 22 |
References_xml | – volume: 67 start-page: 591 issn: 0002-9297 issue: 3 year: 2000 ident: 4_10430050 publication-title: American journal of human genetics doi: 10.1086/303049 contributor: fullname: Yasunaga – volume: 127 start-page: 277 issn: 0092-8674 issue: 2 year: 2006 ident: 5_22876918 publication-title: Cell doi: 10.1016/j.cell.2006.08.040 contributor: fullname: Roux – volume: 4 start-page: e1000207 issn: 1553-7390 issue: 10 year: 2008 ident: 43_32077442 doi: 10.1371/journal.pgen.1000207 contributor: fullname: Hertzano – volume: 369 start-page: 165 issn: 0006-291X issue: 1 year: 2008 ident: 19_30691805 publication-title: Biochemical and biophysical research communications doi: 10.1016/j.bbrc.2007.11.150 contributor: fullname: Buss – volume: 18 start-page: 389 issn: 0959-4388 issue: 4 year: 2008 ident: 10_32061758 publication-title: Current opinion in neurobiology doi: 10.1016/j.conb.2008.09.006 contributor: fullname: Glowatzki – volume: 3 start-page: 851 issn: 1398-9219 issue: 12 year: 2002 ident: 23_17350869 publication-title: Traffic (Copenhagen, Denmark) doi: 10.1034/j.1600-0854.2002.31201.x contributor: fullname: Buss – volume: 563 start-page: 177 issn: 0022-3751 issue: 1 year: 2005 ident: 27_18679199 publication-title: The Journal of Physiology doi: 10.1113/jphysiol.2004.074740 – volume: 53 start-page: 563 issn: 1097-4199 year: 2007 ident: 42_33072860 – volume: 143 start-page: 837 issn: 1873-7544 year: 2006 ident: 57_35238522 – volume: 12 start-page: 483 issn: 0955-0674 issue: 4 year: 2000 ident: 52_10373657 publication-title: Current opinion in cell biology doi: 10.1016/S0955-0674(00)00120-4 contributor: fullname: Mostov – volume: 214 start-page: 331 issn: 0012-1606 issue: 2 year: 1999 ident: 12_11002493 publication-title: Developmental biology doi: 10.1006/dbio.1999.9424 contributor: fullname: Self – volume: 21 start-page: 363 issn: 1061-4036 issue: 4 year: 1999 ident: 1_10774599 publication-title: Nature genetics doi: 10.1038/7693 contributor: fullname: Yasunaga – volume: 284 start-page: 1364 issn: 0021-9258 issue: 3 year: 2009 ident: 6_32604905 publication-title: Journal of Biological Chemistry doi: 10.1074/jbc.M803605200 – volume: 409 start-page: 424 issn: 0021-9967 issue: 3 year: 1999 ident: 48_10862164 publication-title: The Journal of comparative neurology doi: 10.1002/(SICI)1096-9861(19990705)409:3<424::AID-CNE7>3.0.CO;2-L contributor: fullname: Spicer – volume: 277 start-page: 7629 issn: 0021-9258 issue: 10 year: 2002 ident: 39_19552694 publication-title: Journal of Biological Chemistry doi: 10.1074/jbc.R100052200 – volume: 125 start-page: 3709 issn: 0950-1991 issue: 18 year: 1998 ident: 54_6131371 publication-title: Development doi: 10.1242/dev.125.18.3709 contributor: fullname: Knipper – volume: 28 start-page: 7670 issn: 0270-6474 issue: 30 year: 2008 ident: 9_31553663 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.0785-08.2008 contributor: fullname: Johnson – volume: 23 start-page: 10832 issn: 0270-6474 issue: 34 year: 2003 ident: 31_17933084 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.23-34-10832.2003 contributor: fullname: Brandt – volume: 17 start-page: 3814 issn: 0964-6906 issue: 23 year: 2008 ident: 46_31845245 publication-title: Human Molecular Genetics doi: 10.1093/hmg/ddn279 – volume: 69 start-page: 635 issn: 0002-9297 issue: 3 year: 2001 ident: 16_11261253 publication-title: American journal of human genetics doi: 10.1086/323156 contributor: fullname: Melchionda – volume: 24 start-page: 9469 issn: 0270-6474 issue: 43 year: 2004 ident: 26_18492304 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.3162-04.2004 contributor: fullname: Pyott – volume: 27 start-page: 3174 issn: 0270-6474 issue: 12 year: 2007 ident: 8_27936203 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.3965-06.2007 contributor: fullname: Brandt – volume: 312 start-page: 1051 issn: 0036-8075 issue: 5776 year: 2006 ident: 41_21896927 publication-title: Science doi: 10.1126/science.1126308 – volume: 17 start-page: 9133 issn: 0270-6474 issue: 23 year: 1997 ident: 33_5800212 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.17-23-09133.1997 contributor: fullname: Martinez-Dunst – volume: 22 start-page: 569 issn: 0261-4189 issue: 3 year: 2003 ident: 44_17482705 publication-title: The EMBO Journal doi: 10.1093/emboj/cdg055 contributor: fullname: Warner – volume: 101 start-page: 12922 issn: 0027-8424 issue: 35 year: 2004 ident: 24_19687269 publication-title: PNAS doi: 10.1073/pnas.0402660101 – volume: 10 start-page: 3664 issn: 0270-6474 issue: 11 year: 1990 ident: 32_9213711 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.10-11-03664.1990 contributor: fullname: Roberts – volume: 27 start-page: 6442 issn: 0270-6474 issue: 24 year: 2007 ident: 28_28836998 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.5364-06.2007 contributor: fullname: Knirsch – volume: 72 start-page: 1315 issn: 0002-9297 issue: 5 year: 2003 ident: 15_17591918 publication-title: American journal of human genetics doi: 10.1086/375122 contributor: fullname: Ahmed – volume: 40 start-page: 45 issn: 0022-2593 issue: 1 year: 2003 ident: 3_17468585 publication-title: Journal of Medical Genetics doi: 10.1136/jmg.40.1.45 contributor: fullname: Varga – volume: 177 start-page: 103 issn: 0021-9525 issue: 1 year: 2007 ident: 20_27987290 publication-title: The Journal of Cell Biology doi: 10.1083/jcb.200608126 – volume: 22 start-page: 1648 issn: 0270-6474 issue: 5 year: 2002 ident: 36_16945390 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.22-05-01648.2002 contributor: fullname: Meinrenken – volume: 102 start-page: 89 issn: 0092-8674 issue: 1 year: 2000 ident: 30_10418534 publication-title: Cell doi: 10.1016/S0092-8674(00)00013-1 contributor: fullname: Platzer – volume: 97 start-page: 883 issn: 0027-8424 issue: 2 year: 2000 ident: 34_6439082 publication-title: PNAS doi: 10.1073/pnas.97.2.883 contributor: fullname: Moser – volume: 401 start-page: 505 issn: 1476-4687 issue: 6752 year: 1999 ident: 21_10993316 publication-title: Nature; Physical Science (London) doi: 10.1038/46835 contributor: fullname: Wells – volume: 59 start-page: 861 issn: 1097-4199 year: 2008 ident: 38_33072856 – volume: 22 start-page: 5442 issn: 0270-6474 issue: 13 year: 2002 ident: 50_17130275 publication-title: Journal of Neuroscience doi: 20026583 contributor: fullname: Gras – volume: 15 start-page: 1095 issn: 1088-9051 issue: 8 year: 2005 ident: 17_19305831 publication-title: Genome Research doi: 10.1101/gr.3826505 – volume: 24 start-page: 3372 issn: 0953-816X issue: 12 year: 2006 ident: 7_23543378 publication-title: The European journal of neuroscience doi: 10.1111/j.1460-9568.2006.05225.x contributor: fullname: Schug – volume: 83 start-page: 3101 issn: 0022-3077 issue: 5 year: 2000 ident: 55_10310767 publication-title: Journal of Neurophysiology doi: 10.1152/jn.2000.83.5.3101 contributor: fullname: Knipper – volume: 130 start-page: 475 issn: 1873-7544 year: 2005 ident: 25_35205173 – volume: 57 start-page: 536 issn: 1097-4199 year: 2008 ident: 40_35018816 – volume: 10 start-page: 157 issn: 0969-9961 issue: 2 year: 2002 ident: 2_17156732 publication-title: Neurobiology of disease doi: 10.1006/nbdi.2002.0488 contributor: fullname: Mirghomizadeh – volume: 25 start-page: 11577 issn: 0270-6474 issue: 50 year: 2005 ident: 35_21145172 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.3411-05.2005 contributor: fullname: Brandt – volume: 19 start-page: 333 issn: 1081-0706 year: 2003 ident: 53_17867623 publication-title: Annual review of cell and developmental biology doi: 10.1146/annurev.cellbio.19.110701.161425 contributor: fullname: Muth – volume: 28 start-page: 9939 issn: 0270-6474 issue: 40 year: 2008 ident: 45_32029927 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.1124-08.2008 contributor: fullname: Goodyear – volume: 11 start-page: 369 issn: 1061-4036 issue: 4 year: 1995 ident: 11_16094790 publication-title: Nature genetics doi: 10.1038/ng1295-369 contributor: fullname: Avraham – volume: 127 start-page: 425 issn: 0021-9525 issue: 2 year: 1994 ident: 13_15627607 publication-title: The Journal of Cell Biology doi: 10.1083/jcb.127.2.425 contributor: fullname: Hasson – volume: 89 start-page: 445 issn: 0092-8674 issue: 3 year: 1997 ident: 47_16780040 publication-title: Cell doi: 10.1016/S0092-8674(00)80225-1 contributor: fullname: Nakamura – volume: 20 start-page: 649 issn: 1081-0706 year: 2004 ident: 22_18464596 publication-title: Annual review of cell and developmental biology doi: 10.1146/annurev.cellbio.20.012103.094243 contributor: fullname: Buss – volume: 22 start-page: 3939 issn: 0270-6474 issue: 10 year: 2002 ident: 51_17060033 publication-title: Journal of Neuroscience doi: 20026397 contributor: fullname: Griesinger – volume: 234 start-page: 21 issn: 0378-5955 issue: 1-2 year: 2007 ident: 18_29904491 publication-title: Hearing research doi: 10.1016/j.heares.2007.09.005 contributor: fullname: Longo-Guess – volume: 5 start-page: 798 issn: 1398-9219 issue: 10 year: 2004 ident: 29_18430315 publication-title: Traffic (Copenhagen, Denmark) doi: 10.1111/j.1600-0854.2004.00224.x contributor: fullname: Dance – volume: 17 start-page: 6961 issn: 0270-6474 issue: 18 year: 1997 ident: 37_5720563 publication-title: Journal of Neuroscience doi: 10.1523/JNEUROSCI.17-18-06961.1997 contributor: fullname: Naraghi – volume: 19 start-page: 2059 issn: 1059-1524 issue: 5 year: 2008 ident: 49_30494199 publication-title: Molecular Biology of the Cell doi: 10.1091/mbc.E07-09-0902 – volume: 20 start-page: 3676 issn: 0261-4189 issue: 14 year: 2001 ident: 56_11229865 publication-title: The EMBO Journal doi: 10.1093/emboj/20.14.3676 contributor: fullname: Buss – volume: 6 start-page: 1225 issn: 0964-6906 issue: 8 year: 1997 ident: 14_16878183 publication-title: Human Molecular Genetics doi: 10.1093/hmg/6.8.1225 contributor: fullname: Avraham |
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Snippet | Otoferlin has been proposed to be the Ca2+ sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and... Otoferlin has been proposed to be the Ca(2+) sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and... Otoferlin has been proposed to be the Ca[sup]2+ sensor in hair cell exocytosis, compensating for the classical synaptic fusion proteins synaptotagmin-1 and... |
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SubjectTerms | Amino Acid Sequence Animals Biological and medical sciences Calcium - metabolism Deafness - genetics Deafness - metabolism Disease Models, Animal Exocytosis Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Hair Cells, Auditory, Inner - chemistry Hair Cells, Auditory, Inner - metabolism Humans Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mice, Transgenic Molecular and cellular biology Molecular Sequence Data Myosin Heavy Chains - chemistry Myosin Heavy Chains - genetics Myosin Heavy Chains - metabolism Protein Binding Protein Transport Synapses - chemistry Synapses - metabolism Two-Hybrid System Techniques |
Title | Otoferlin interacts with myosin VI: implications for maintenance of the basolateral synaptic structure of the inner hair cell |
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