Isolation and characterization of PRA1, a trypsin-like protease from the biocontrol agent Trichoderma harzianum CECT 2413 displaying nematicidal activity

• Published in: Applied microbiology and biotechnology Vol. 65; no. 1; pp. 46 - 55
• Main Authors: Suarez, B, Rey, M, Castillo, P, Monte, E, Llobell, A
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.07.2004; Springer Nature B.V
• Subjects: Amino Acid Sequence; analysis; Animals; Antinematodal Agents; Antinematodal Agents - isolation & purification; Antinematodal Agents - pharmacology; Biological and medical sciences; Biological control; biological control agents; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Cell culture; Cell walls; Chitin; classification; Culture media; Derepression; Endopeptidases; Endopeptidases - analysis; Endopeptidases - classification; Endopeptidases - isolation & purification; Endopeptidases - pharmacology; Enzymes; enzymology; Fundamental and applied biological sciences. Psychology; Fungi; genetics; Homogeneity; isolation & purification; Meloidogyne incognita; methods; microbiology; Miscellaneous; Mission oriented research; Molecular Sequence Data; nematicidal properties; Nematoda; Oligonucleotides; Pest control; Pest Control, Biological; Pest Control, Biological - methods; Pests; pharmacology; Phylogeny; Plant Diseases; Plant Diseases - microbiology; plant parasitic nematodes; Protease; proteinases; Proteins; Proteolysis; Substrate inhibition; Substrate specificity; Synergistic effect; Trichoderma; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma harzianum; Trypsin; Nematicide; Serine endopeptidases; Enzyme; Biological control; Pesticides; Biological agent; Fungi; Characterization; Peptidases; Trichoderma harzianum; Trypsin; Hydrolases; Isolation; Fungi Imperfecti; Thallophyta

Mycoparasitic Trichoderma strains secrete a complex set of hydrolytic enzymes under conditions related to antagonism. Several proteins with proteolytic activity were detected in culture filtrates from T. harzianum CECT 2413 grown in fungal cell walls or chitin and the protein responsible for the mai...