Trimmomatic: a flexible trimmer for Illumina sequence data

• Published in: Bioinformatics (Oxford, England) Vol. 30; no. 15; pp. 2114 - 2120
• Main Authors: Bolger, Anthony M., Lohse, Marc, Usadel, Bjoern
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.08.2014
• Subjects: Computational Biology; Databases, Genetic; High-Throughput Nucleotide Sequencing - methods; Original Papers; Software
• ISSN: 1367-4803; 1367-4811; 1367-4811
• DOI: 10.1093/bioinformatics/btu170

Motivation: Although many next-generation sequencing (NGS) read preprocessing tools already existed, we could not find any tool or combination of tools that met our requirements in terms of flexibility, correct handling of paired-end data and high performance. We have developed Trimmomatic as a more...