Speciation of coagulase negative staphylococci, isolated from indoor air, using SDS page gel bands of expressed proteins followed by MALDI TOF MS and MALDI TOF-TOF MS-MS analysis of tryptic peptides

• Published in: Journal of microbiological methods Vol. 84; no. 2; pp. 243 - 250
• Main Authors: Fox, Karen, Fox, Alvin, Rose, John, Walla, Michael
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.2011; Elsevier
• Subjects: aconitate hydratase; air; Air Microbiology; Bacterial Proteins - analysis; Bacteriological methods and techniques used in bacteriology; Bacteriological Techniques - methods; Bacteriology; Biological and medical sciences; coagulase negative staphylococci; conserved sequences; digestion; Electrophoresis, Polyacrylamide Gel - methods; environmental assessment; Fundamental and applied biological sciences. Psychology; genome; MALDI-TOF MS; MALDI-TOFTOF MS-MS; Mass spectrometry; Microbiology; peptides; polyacrylamide gel electrophoresis; protein synthesis; Proteome - analysis; Proteomics; Schools; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Staphylococcus - chemistry; Staphylococcus - classification; Staphylococcus - isolation & purification; Tandem mass spectrometry; MALDI-TOFTOF MS-MS; Tandem mass spectrometry; MALDI-TOF MS; Mass spectrometry; Proteomics; Peptides; Protein; Staphylococcus; Mass spectrometry MS/MS; Bacteria; Micrococcales; Micrococcaceae; Speciation

A group of coagulase negative staphylococcal strains isolated from indoor air of occupied school rooms were the subject of this study. Conventional MALDI TOF MS profiling of cellular extracts and physiological tests (including API STAPH) provided incomplete identification of the set of strains. Afte...