Determination of Serum Lipid Peroxide Levels in Healthy and Diseased Subjects Use of a Methylene Blue Derivative
By using a newly developed assay method for lipid peroxides based on their reaction with a leucomethylene blue derivative, we investigated serum lipid peroxides (LPO) in healthy and in diseased subjects. In 116 healthy subjects, the mean LPO level obtained was 1.45±1.37nmol/ml and showed significant...
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| Published in | Journal of Clinical Biochemistry and Nutrition Vol. 7; no. 2; pp. 169 - 174 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Gifu
SOCIETY FOR FREE RADICAL RESEARCH JAPAN
1989
Japan Science and Technology Agency |
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| ISSN | 0912-0009 1880-5086 |
| DOI | 10.3164/jcbn.7.169 |
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| Abstract | By using a newly developed assay method for lipid peroxides based on their reaction with a leucomethylene blue derivative, we investigated serum lipid peroxides (LPO) in healthy and in diseased subjects. In 116 healthy subjects, the mean LPO level obtained was 1.45±1.37nmol/ml and showed significant correlations with total bilirubin, direct bilirubin, phospholipid, total cholesterol, high density lipoprotein-cholesterol, and low density lipoprotein-cholesterol. In 12 hypertensive patients, the LPO value was significantly higher (2.60±2.08nmol/ml) compared with the healthy control, and LPO showed a significant correlation with serum globulin. In 16 hyperlipidemic subjects, the value was 1.50±0.86nmol/ml and correlated with fibrinogen, white blood cells, and nonesterified fatty acids. In 19 diabetics, the level was not significantly different with that in the healthy control, and correlated with fasting blood sugar, total protein, and urea nitrogen. In 10 patients with liver dysfunction, LPO was 1.60±1.33nmol/ml and correlated with white blood cells, chloride, and nonesterified fatty acids. |
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| AbstractList | By using a newly developed assay method for lipid peroxides based on their reaction with a leucomethylene blue derivative, we investigated serum lipid peroxides (LPO) in healthy and in diseased subjects. In 116 healthy subjects, the mean LPO level obtained was 1.45±1.37nmol/ml and showed significant correlations with total bilirubin, direct bilirubin, phospholipid, total cholesterol, high density lipoprotein-cholesterol, and low density lipoprotein-cholesterol. In 12 hypertensive patients, the LPO value was significantly higher (2.60±2.08nmol/ml) compared with the healthy control, and LPO showed a significant correlation with serum globulin. In 16 hyperlipidemic subjects, the value was 1.50±0.86nmol/ml and correlated with fibrinogen, white blood cells, and nonesterified fatty acids. In 19 diabetics, the level was not significantly different with that in the healthy control, and correlated with fasting blood sugar, total protein, and urea nitrogen. In 10 patients with liver dysfunction, LPO was 1.60±1.33nmol/ml and correlated with white blood cells, chloride, and nonesterified fatty acids. |
| Author | TATEISHI, Tohru YOSHIMINE, Noboru KUZUYA, Fumio |
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| References | 4. Suematsu, T., Kaneda, T., Abe, H., Kikuchi, S., and Yagi, K. (1977): Serum lipoperoxide level in patients suffering from liver diseases. Clin. Chim. Acta, 79, 267-270. 7. Yagi, K. (1976): A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Med., 15, 212-216. 17. Poubelle, P., Chaintreuil, J., Bensadoun, F., Blotman, F., Simon, L., and De Paulet, A.C. (1982): Plasma lipoperoxides and aging. Biomedicine, 36, 164-166. 12. Tateishi, T., Yoshimine, N., and Kuzuya, F. (1988): Serum lipid peroxide levels and their distributions in the lipoprotein fractions by a new method. J. Jpn. Atheroscler. Soc., 15, 1585-1586. 14. Yagi, K., Yamauchi, T., Kobayashi, S., Komura, S., Ohishi, N., Kayahara, N., and Tatano, T. (1987): Examination of lipid peroxide assay by the hemoglobin-methylene blue method. Lipid Peroxide Res., 11, 38. 15. Yoshida, K., Kaku, H., Nakajima, S., Okazaki, K., and Inaba, G. (1987): Correlation between serum lipid peroxide and serum iron in rheumatoid arthritis determined by a new Yagi colorimetric method. Igakunoayumi, 140, 171-172. 2. Harman, D. (1982): The free-radical theory of aging, in Free Radicals in Biology, ed. by Pryor, W. A., Academic Press, New York, Vol. 5, pp. 255-275. 3. Iwakami, M. (1965): Peroxides as a factor of atherosclerosis. Nagoya J. Med. Sci., 28, 50-66. 1. Pryor, W. A. (1976): The role of free radical reactions in biological systems, in Free Radicals in Biology, ed. by Pryor, W. A., Academic Press, New York, Vol. 1, pp. 1-49. 10. Yagi, K., Kiuchi, K., Saito, Y., Miike, A., Kayahara, N., Tatano, T., and Ohishi, N. (1986): Use of a new methylene blue derivative for determination of lipid hydroperoxide in foods. Biochem. Int., 12, 367-371. 16. Yoshimine, N., Sato, Y., and Kuzuya, F. (1977): Lipid peroxide and aging, with special reference to vascular damage. Jpn. J. Geriatr., 15, 201-204. 9. Ohishi, N., Ohkawa, H., Miike, A., Tatano, T., and Yagi, K. (1985): A new assay method for lipid peroxides using a methylene blue derivative. Biochem. Int., 10, 205-211. 11. Tanaka, Y., Nishikawa, Y., Utagawa, A., Horikawa, M., Tomotake, A., Suganuma, H., Kanda, T., and Suematsu, T. (1986): Lipid peroxide measurement by the enzyme method and its clinical significance. Rinshobyori, 34, 120. 6. Asakawa, T., and Matsushita, S. (1980): Thiobarbituric acid test for detecting lipid peroxides. Lipids, 14, 401-406. 5. Sato, Y., Hotta, N., Sakamoto, N., Matsuoka, S., Ohishi, N., and Yagi, K. (1979): Lipid peroxide level in plasma of diabetic patients. Biochem. Med., 21, 104-107. 13. Tateishi, T., Yoshimine, N., and Kuzuya, F. (1987): Serum lipid peroxide assayed by a new colorimetric method. Exp. Gerontol., 22, 103-111. 8. Ohkawa, H., Ohishi, N., and Yagi, K. (1978): Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J. Lipid Res., 19, 1053-1057. 18. Hata, Y., Aihara, K., and Miyazaki, K. (1978): A study of the relation between atherosclerosis and lipid peroxide. Jpn. J. Geriatr., 15, 192-200. |
| References_xml | – reference: 5. Sato, Y., Hotta, N., Sakamoto, N., Matsuoka, S., Ohishi, N., and Yagi, K. (1979): Lipid peroxide level in plasma of diabetic patients. Biochem. Med., 21, 104-107. – reference: 1. Pryor, W. A. (1976): The role of free radical reactions in biological systems, in Free Radicals in Biology, ed. by Pryor, W. A., Academic Press, New York, Vol. 1, pp. 1-49. – reference: 10. Yagi, K., Kiuchi, K., Saito, Y., Miike, A., Kayahara, N., Tatano, T., and Ohishi, N. (1986): Use of a new methylene blue derivative for determination of lipid hydroperoxide in foods. Biochem. Int., 12, 367-371. – reference: 18. Hata, Y., Aihara, K., and Miyazaki, K. (1978): A study of the relation between atherosclerosis and lipid peroxide. Jpn. J. Geriatr., 15, 192-200. – reference: 2. Harman, D. (1982): The free-radical theory of aging, in Free Radicals in Biology, ed. by Pryor, W. A., Academic Press, New York, Vol. 5, pp. 255-275. – reference: 17. Poubelle, P., Chaintreuil, J., Bensadoun, F., Blotman, F., Simon, L., and De Paulet, A.C. (1982): Plasma lipoperoxides and aging. Biomedicine, 36, 164-166. – reference: 7. Yagi, K. (1976): A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Med., 15, 212-216. – reference: 13. Tateishi, T., Yoshimine, N., and Kuzuya, F. (1987): Serum lipid peroxide assayed by a new colorimetric method. Exp. Gerontol., 22, 103-111. – reference: 6. Asakawa, T., and Matsushita, S. (1980): Thiobarbituric acid test for detecting lipid peroxides. Lipids, 14, 401-406. – reference: 8. Ohkawa, H., Ohishi, N., and Yagi, K. (1978): Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J. Lipid Res., 19, 1053-1057. – reference: 14. Yagi, K., Yamauchi, T., Kobayashi, S., Komura, S., Ohishi, N., Kayahara, N., and Tatano, T. (1987): Examination of lipid peroxide assay by the hemoglobin-methylene blue method. Lipid Peroxide Res., 11, 38. – reference: 11. Tanaka, Y., Nishikawa, Y., Utagawa, A., Horikawa, M., Tomotake, A., Suganuma, H., Kanda, T., and Suematsu, T. (1986): Lipid peroxide measurement by the enzyme method and its clinical significance. Rinshobyori, 34, 120. – reference: 16. Yoshimine, N., Sato, Y., and Kuzuya, F. (1977): Lipid peroxide and aging, with special reference to vascular damage. Jpn. J. Geriatr., 15, 201-204. – reference: 15. Yoshida, K., Kaku, H., Nakajima, S., Okazaki, K., and Inaba, G. (1987): Correlation between serum lipid peroxide and serum iron in rheumatoid arthritis determined by a new Yagi colorimetric method. Igakunoayumi, 140, 171-172. – reference: 4. Suematsu, T., Kaneda, T., Abe, H., Kikuchi, S., and Yagi, K. (1977): Serum lipoperoxide level in patients suffering from liver diseases. Clin. Chim. Acta, 79, 267-270. – reference: 9. Ohishi, N., Ohkawa, H., Miike, A., Tatano, T., and Yagi, K. (1985): A new assay method for lipid peroxides using a methylene blue derivative. Biochem. Int., 10, 205-211. – reference: 3. Iwakami, M. (1965): Peroxides as a factor of atherosclerosis. Nagoya J. Med. Sci., 28, 50-66. – reference: 12. Tateishi, T., Yoshimine, N., and Kuzuya, F. (1988): Serum lipid peroxide levels and their distributions in the lipoprotein fractions by a new method. J. Jpn. Atheroscler. Soc., 15, 1585-1586. |
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| Title | Determination of Serum Lipid Peroxide Levels in Healthy and Diseased Subjects Use of a Methylene Blue Derivative |
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