Determination of Serum Lipid Peroxide Levels in Healthy and Diseased Subjects Use of a Methylene Blue Derivative

• Published in: Journal of Clinical Biochemistry and Nutrition Vol. 7; no. 2; pp. 169 - 174
• Main Authors: TATEISHI, Tohru, KUZUYA, Fumio, YOSHIMINE, Noboru
• Format: Journal Article
• Language: English
• Published: Gifu SOCIETY FOR FREE RADICAL RESEARCH JAPAN 1989; Japan Science and Technology Agency
• Subjects: Cholesterol; clinical parameters; Fatty acids; lipid peroxides; methylene blue derivative; Urea
• ISSN: 0912-0009; 1880-5086
• DOI: 10.3164/jcbn.7.169

By using a newly developed assay method for lipid peroxides based on their reaction with a leucomethylene blue derivative, we investigated serum lipid peroxides (LPO) in healthy and in diseased subjects. In 116 healthy subjects, the mean LPO level obtained was 1.45±1.37nmol/ml and showed significant correlations with total bilirubin, direct bilirubin, phospholipid, total cholesterol, high density lipoprotein-cholesterol, and low density lipoprotein-cholesterol. In 12 hypertensive patients, the LPO value was significantly higher (2.60±2.08nmol/ml) compared with the healthy control, and LPO showed a significant correlation with serum globulin. In 16 hyperlipidemic subjects, the value was 1.50±0.86nmol/ml and correlated with fibrinogen, white blood cells, and nonesterified fatty acids. In 19 diabetics, the level was not significantly different with that in the healthy control, and correlated with fasting blood sugar, total protein, and urea nitrogen. In 10 patients with liver dysfunction, LPO was 1.60±1.33nmol/ml and correlated with white blood cells, chloride, and nonesterified fatty acids.