Divergent Impact of Actin Isoforms on Division of Epithelial Cells
We investigated distribution and functions of beta- and gamma-cytoplasmic actins (CYAs) at different stages of non-neoplastic epithelial cell division using laser scanning microscopy (LSM). Here, we demonstrated that beta- and gamma-CYAs are spatially segregated in the early prophase, anaphase, telo...
Saved in:
| Published in | Biochemistry (Moscow) Vol. 85; no. 9; pp. 1072 - 1081 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Moscow
Pleiades Publishing
01.09.2020
Springer |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | We investigated distribution and functions of beta- and gamma-cytoplasmic actins (CYAs) at different stages of non-neoplastic epithelial cell division using laser scanning microscopy (LSM). Here, we demonstrated that beta- and gamma-CYAs are spatially segregated in the early prophase, anaphase, telophase, and cytokinesis. Small interfering RNA (siRNA) experiments revealed that in both beta-CYA- and gamma-CYA-depleted cells, the number of cells was significantly reduced compared with the siRNA controls. Beta-CYA depletion resulted in an enlargement of the cell area in metaphase and high percentage of polynuclear cells compared with the siRNA control, indicating a potential failure of cytokinesis. Gamma-CYA depletion resulted in a reduced percentage of mitotic cells. We also observed the interdependence between the actin isoforms and the microtubule system in mitosis: (i) a decrease in the gamma-CYA led to impaired mitotic spindle organization; (ii) suppression of tubulin polymerization caused impaired beta-CYA reorganization, as incubation with colcemid blocked the transfer of short beta-actin polymers from the basal to the cortical compartment. We conclude that both actin isoforms are essential for proper cell division, but each isoform has its own specific functional role in this process. |
|---|---|
| AbstractList | We investigated distribution and functions of beta- and gamma-cytoplasmic actins (CYAs) at different stages of non-neoplastic epithelial cell division using laser scanning microscopy (LSM). Here, we demonstrated that beta- and gamma-CYAs are spatially segregated in the early prophase, anaphase, telophase, and cytokinesis. Small interfering RNA (siRNA) experiments revealed that in both beta-CYA- and gamma-CYA-depleted cells, the number of cells was significantly reduced compared with the siRNA controls. Beta-CYA depletion resulted in an enlargement of the cell area in metaphase and high percentage of polynuclear cells compared with the siRNA control, indicating a potential failure of cytokinesis. Gamma-CYA depletion resulted in a reduced percentage of mitotic cells. We also observed the interdependence between the actin isoforms and the microtubule system in mitosis: (i) a decrease in the gamma-CYA led to impaired mitotic spindle organization; (ii) suppression of tubulin polymerization caused impaired beta-CYA reorganization, as incubation with colcemid blocked the transfer of short beta-actin polymers from the basal to the cortical compartment. We conclude that both actin isoforms are essential for proper cell division, but each isoform has its own specific functional role in this process. |
| Audience | Academic |
| Author | Alieva, I. B. Chaponnier, C. Dugina, V. B. Shagieva, G. S. |
| Author_xml | – sequence: 1 givenname: G. S. surname: Shagieva fullname: Shagieva, G. S. organization: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University – sequence: 2 givenname: I. B. surname: Alieva fullname: Alieva, I. B. email: irina_alieva@belozersky.msu.ru organization: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Federal Research and Clinical Center of Physical-Chemical Medicine of the Federal Medical Biological Agency – sequence: 3 givenname: C. surname: Chaponnier fullname: Chaponnier, C. organization: Department of Pathology and Immunology, Faculty of Medicine, University of Geneva – sequence: 4 givenname: V. B. surname: Dugina fullname: Dugina, V. B. organization: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33050852$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kU1rGzEQhkVJaBw3P6CXstBLLpuMPla7OrpuPgyGHJKehSyPXIVdyZXWhfz7yDgplISggzR6n3cY3jklRyEGJOQrhQtKubi8BwDJVKsYgAJo2ScyoRK6moOAIzLZy_VePyGnOT-WkoHin8kJ59BA17AJ-fHT_8W0wTBWi2Fr7FhFV83s6EO1yNHFNOQqhqpQPvvyKOrV1o-_sfemr-bY9_kLOXamz3j2ck_Jr-urh_ltvby7Wcxny9qKth1r2XTKyY4CVQalaa1gYFawFkI23LRSWmtXQim3ggadY80a0HJpKHZMtJLyKTk_9N2m-GeHedSDz7ZMYALGXdZMNCWVEoQq6PcDujE9ah9cHJOxe1zPJJdMqoZ1hbp4hypnjYO3JWrny_9_Bnow2BRzTuj0NvnBpCdNQe83ot9spHi-vUy9Ww24_ud4XUEB2AHIRQobTPox7lIoSX7Q9RnmUJNp |
| CitedBy_id | crossref_primary_10_3390_molecules26082151 crossref_primary_10_1016_j_ejcb_2023_151315 crossref_primary_10_1016_j_jdermsci_2022_01_006 crossref_primary_10_3389_fphar_2022_895703 crossref_primary_10_3389_fcell_2022_1007614 crossref_primary_10_3390_ijms22157836 |
| Cites_doi | 10.1002/cm.21071 10.1016/j.cub.2007.11.068 10.1080/15384101.2018.1553337 10.1126/science.1081412 10.1038/s41467-017-01231-x 10.1073/pnas.0603704103 10.1083/jcb.34.2.535 10.1038/sj.onc.1203987 10.1083/jcb.34.2.525 10.1038/s41580-018-0067-1 10.1095/biolreprod.111.091736 10.1016/j.cub.2020.02.023 10.1126/science.aal1647 10.1242/jcs.041970 10.1016/j.devcel.2011.07.018 10.1016/s0248-4900(02)01202-9 10.1016/j.devcel.2006.12.010 10.1007/164_2016_43 10.18632/oncotarget.12236 10.1080/15384101.2015.1120920 10.1098/rstb.2000.0632 10.1083/jcb.201603025 10.1074/jbc.M405319200 10.1016/j.devcel.2013.03.014 10.1002/bies.20477 10.1016/S0092-8674(04)00341-1 10.1083/jcb.106.3.761 10.1038/nature01148 10.1101/cshperspect.a018267 10.18632/oncotarget.3989 10.1016/0092-8674(92)90163-7 10.1201/9780203833445 |
| ContentType | Journal Article |
| Copyright | Pleiades Publishing, Ltd. 2020 COPYRIGHT 2020 Springer |
| Copyright_xml | – notice: Pleiades Publishing, Ltd. 2020 – notice: COPYRIGHT 2020 Springer |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
| DOI | 10.1134/S0006297920090072 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
| DatabaseTitleList | MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry Biology |
| EISSN | 1608-3040 |
| EndPage | 1081 |
| ExternalDocumentID | A636269528 10_1134_S0006297920090072 33050852 |
| Genre | Journal Article |
| GeographicLocations | Germany |
| GeographicLocations_xml | – name: Germany |
| GroupedDBID | -56 -5G -BR -EM -Y2 -~C -~X .86 .VR 06C 06D 0R~ 0VY 1N0 23N 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 36B 3V. 4.4 408 409 40D 40E 5GY 5VS 67N 67Z 6J9 6NX 78A 7X7 88A 88E 88I 8AO 8C1 8CJ 8FE 8FH 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X A8Z AAAVM AABHQ AAFGU AAHNG AAIAL AAJKR AANXM AANZL AAPBV AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABBBX ABBXA ABDBF ABDZT ABECU ABFGW ABFTV ABHLI ABHQN ABJNI ABJOX ABKAS ABKCH ABKTR ABMNI ABMQK ABNWP ABPTK ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFO ACGFS ACGOD ACHSB ACHXU ACIGE ACIPQ ACKNC ACMDZ ACMLO ACOKC ACOMO ACPRK ACREN ACTTH ACVWB ACWMK ADBBV ADHHG ADHIR ADINQ ADKNI ADKPE ADMDM ADOXG ADRFC ADTPH ADURQ ADYFF ADYOE ADZKW AEBTG AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESTI AETLH AEVLU AEVTX AEXYK AFFNX AFGCZ AFKRA AFLOW AFNRJ AFQWF AFRAH AFWTZ AFYQB AFZKB AGAYW AGDGC AGGBP AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMTXH AMXSW AMYLF AMYQR AOCGG ARMRJ ASPBG AVWKF AXYYD AZFZN AZQEC B-. B0M BA0 BBNVY BDATZ BENPR BGNMA BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CSCUP D1J DDRTE DL5 DNIVK DPUIP DU5 DWQXO EAD EAP EBD EBLON EBS EIOEI EJD EMB EMK EMOBN EN4 EPL ESBYG ESTFP ESX F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNUQQ GNWQR GQ6 GQ7 GQ8 GX1 GXS HCIFZ HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ H~9 IAO IEA IHE IHR IJ- IKXTQ INH INR ITC ITM IWAJR IXC IZIGR I~X I~Z J-C JBSCW JCJTX JZLTJ KDC KOV KPH LAK LK8 LLZTM M0L M1P M2P M4Y M7P MA- N2Q NB0 NPVJJ NQJWS NU0 O9- O93 O9I O9J OAM OVD P2P PF0 PQQKQ PROAC PSQYO PT4 Q2X QOR QOS R89 R9I RNI RNS ROL RPX RSV RZC RZE S16 S1Z S27 S3A S3B SAP SBL SDH SHX SISQX SJN SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW STPWE SV3 SZN T13 TEORI TSG TSK TSV TUC TUS U2A U9L UG4 UKHRP UNUBA UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WH7 WJK WK8 XU3 YLTOR Z7U Z7V Z7W Z87 ZGI ZMTXR ZOVNA ~8M ~A9 ~KM AACDK AAJBT AASML AAYZH ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AGRTI AIGIU ALIPV CGR CUY CVF ECM EIF H13 NPM AAYXX ABQSL CITATION 7X8 |
| ID | FETCH-LOGICAL-c477t-6589f681019ae6a7c420ab0d44653a766cccb499fb05eff25d0ec36a1e8247613 |
| IEDL.DBID | AGYKE |
| ISSN | 0006-2979 |
| IngestDate | Fri Oct 25 11:36:44 EDT 2024 Tue Nov 19 21:03:36 EST 2024 Tue Nov 12 23:01:58 EST 2024 Fri Dec 06 02:42:16 EST 2024 Wed Oct 16 00:44:15 EDT 2024 Sat Dec 16 12:02:19 EST 2023 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Keywords | cell division microtubules cytoplasmic actin mitosis |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c477t-6589f681019ae6a7c420ab0d44653a766cccb499fb05eff25d0ec36a1e8247613 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 33050852 |
| PQID | 2451130909 |
| PQPubID | 23479 |
| PageCount | 10 |
| ParticipantIDs | proquest_miscellaneous_2451130909 gale_infotracmisc_A636269528 gale_infotracacademiconefile_A636269528 crossref_primary_10_1134_S0006297920090072 pubmed_primary_33050852 springer_journals_10_1134_S0006297920090072 |
| PublicationCentury | 2000 |
| PublicationDate | 2020-09-01 |
| PublicationDateYYYYMMDD | 2020-09-01 |
| PublicationDate_xml | – month: 09 year: 2020 text: 2020-09-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | Moscow |
| PublicationPlace_xml | – name: Moscow – name: United States |
| PublicationTitle | Biochemistry (Moscow) |
| PublicationTitleAbbrev | Biochemistry Moscow |
| PublicationTitleAlternate | Biochemistry (Mosc) |
| PublicationYear | 2020 |
| Publisher | Pleiades Publishing Springer |
| Publisher_xml | – name: Pleiades Publishing – name: Springer |
| References | Etienne-Manneville, Hall (CR9) 2002; 420 Mogessie, Schuh (CR21) 2017; 357 Po’uha, Kavallaris (CR15) 2015; 14 Chen, Wang, Svitkina, Bi (CR23) 2020; 30 Uzbekov, Kireyev, Prigent (CR18) 2002; 94 Lancaster, LeBerre, Dimitracopoulos, Bonazzi, Zlotek-Zlotkiewicz, Picone, Duke, Piel, Baum (CR19) 2013; 25 Borisy, Taylor (CR12) 1967; 34 Borisy, Taylor (CR13) 1967; 34 Jordan, Canman (CR22) 2012; 69 Dugina, Zwaenepoel, Gabbiani, Clement, Chaponnier, Clément, Chaponnier (CR2) 2009; 122 Svitkina (CR24) 2018; 10 Xiao, Verdier-Pinard, Fernandez-Fuentes, Burd, Angeletti, Fiser, Horwitz, Orr (CR11) 2006; 103 Takaishi, Sasaki, Kameyama, Tsukita, Tsukita, Takai (CR28) 1995; 11 Bement, Miller, Von Dassow (CR10) 2006; 28 Piekny, Glotzer (CR25) 2008; 18 Ridley, Hall (CR26) 1992; 70 Sandquist, Kita, Bement (CR17) 2011; 21 Dugina, Alieva, Khromova, Kireev, Gunning, Kopnin (CR3) 2016; 7 Rosenblatt, Cramer, Baum, McGee (CR20) 2004; 117 Kosako, Yoshida, Matsumura, Ishizaki, Narumiya, Inagaki (CR29) 2000; 19 CR7 Halet, Carroll (CR31) 2007; 12 Dogterom, Koenderink (CR4) 2019; 20 Boukamp, Petrussevska, Breitkreutz, Hornung, Markham, Fusenig (CR5) 1988; 106 Brockmann, Huarte, Dugina, Challet, Rey, Conne, Swetloff, Nef, Chaponnier, Vassalli (CR14) 2011; 85 Kovács, Tóth, Hetényi, Málnási-Csizmadia, Sellers (CR6) 2004; 279 Dugina, Shagieva, Khromova, Kopnin (CR33) 2018; 17 Wagner, Glotzer (CR27) 2016; 213 Dugina, Khromova, Rybko, Blizniukov, Shagieva, Chaponnier, Kopnin, Kopnin (CR32) 2015; 6 Chen, Arora, McCulloch, Wilde (CR16) 2017; 8 Ampe, Van Troys (CR1) 2017; 235 Straight, Cheung, Limouze, Chen, Westwood, Sellers, Mitchison (CR8) 2003; 299 Hall, Nobes (CR30) 2000; 355 G. Halet (2063_CR31) 2007; 12 M. Dogterom (2063_CR4) 2019; 20 V. Dugina (2063_CR2) 2009; 122 K. Takaishi (2063_CR28) 1995; 11 A. F. Straight (2063_CR8) 2003; 299 S. T. Po’uha (2063_CR15) 2015; 14 R. Uzbekov (2063_CR18) 2002; 94 E. Wagner (2063_CR27) 2016; 213 J. C. Sandquist (2063_CR17) 2011; 21 V. Dugina (2063_CR3) 2016; 7 W. M. Bement (2063_CR10) 2006; 28 B. Mogessie (2063_CR21) 2017; 357 C. Brockmann (2063_CR14) 2011; 85 V. Dugina (2063_CR33) 2018; 17 S. Etienne-Manneville (2063_CR9) 2002; 420 A. J. Ridley (2063_CR26) 1992; 70 2063_CR7 H. Xiao (2063_CR11) 2006; 103 X. Chen (2063_CR23) 2020; 30 A. Chen (2063_CR16) 2017; 8 H. Kosako (2063_CR29) 2000; 19 G. G. Borisy (2063_CR13) 1967; 34 V. Dugina (2063_CR32) 2015; 6 C. Ampe (2063_CR1) 2017; 235 S. N. Jordan (2063_CR22) 2012; 69 J. Rosenblatt (2063_CR20) 2004; 117 A. Hall (2063_CR30) 2000; 355 G. G. Borisy (2063_CR12) 1967; 34 M. Kovács (2063_CR6) 2004; 279 A. J. Piekny (2063_CR25) 2008; 18 T. Svitkina (2063_CR24) 2018; 10 P. Boukamp (2063_CR5) 1988; 106 O. Lancaster (2063_CR19) 2013; 25 |
| References_xml | – volume: 69 start-page: 919 year: 2012 end-page: 930 ident: CR22 article-title: Rho GTPases in animal cell cytokinesis: an occupation by the one percent publication-title: Cytoskeleton (Hoboken) doi: 10.1002/cm.21071 contributor: fullname: Canman – volume: 18 start-page: 30 year: 2008 end-page: 36 ident: CR25 article-title: Anillin is a scaffold protein that links RhoA, actin, and myosin during cytokinesis publication-title: Curr. Biol. doi: 10.1016/j.cub.2007.11.068 contributor: fullname: Glotzer – volume: 17 start-page: 2610 year: 2018 end-page: 2621 ident: CR33 article-title: Divergent impact of actin isoforms on cell cycle regulation publication-title: Cell Cycle doi: 10.1080/15384101.2018.1553337 contributor: fullname: Kopnin – volume: 299 start-page: 1743 year: 2003 end-page: 1747 ident: CR8 article-title: Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor publication-title: Science doi: 10.1126/science.1081412 contributor: fullname: Mitchison – volume: 8 start-page: 1530 year: 2017 ident: CR16 article-title: Cytokinesis requires localized β-actin filament production by an actin isoform specific nucleator publication-title: Nat. Commun. doi: 10.1038/s41467-017-01231-x contributor: fullname: Wilde – volume: 103 start-page: 10166 year: 2006 end-page: 10173 ident: CR11 article-title: Insights into the mechanism of microtubule stabilization by taxol publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.0603704103 contributor: fullname: Orr – volume: 34 start-page: 535 year: 1967 end-page: 548 ident: CR12 article-title: The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus publication-title: J. Cell Biol. doi: 10.1083/jcb.34.2.535 contributor: fullname: Taylor – volume: 19 start-page: 6059 year: 2000 end-page: 6064 ident: CR29 article-title: Rho-kinase/ROCK is involved in cytokinesis through the phosphorylation of myosin light chain and not ezrin/radixin/moesin proteins at the cleavage furrow publication-title: Oncogene doi: 10.1038/sj.onc.1203987 contributor: fullname: Inagaki – volume: 34 start-page: 525 year: 1967 end-page: 533 ident: CR13 article-title: The mechanism action of colchicine. Binding of colchincine-3H to cellular protein publication-title: J. Cell Biol. doi: 10.1083/jcb.34.2.525 contributor: fullname: Taylor – volume: 20 start-page: 38 year: 2019 end-page: 54 ident: CR4 article-title: Actin–microtubule crosstalk in cell biology publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/s41580-018-0067-1 contributor: fullname: Koenderink – volume: 85 start-page: 1025 year: 2011 end-page: 1039 ident: CR14 article-title: Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes publication-title: Biol. Reprod. doi: 10.1095/biolreprod.111.091736 contributor: fullname: Vassalli – volume: 30 start-page: 1477 year: 2020 end-page: 1490.e3 ident: CR23 article-title: Critical roles of a RhoGEF-anillin module in septin architectural remodeling during cytokinesis publication-title: Curr. Biol. doi: 10.1016/j.cub.2020.02.023 contributor: fullname: Bi – volume: 357 start-page: eaal1647 year: 2017 ident: CR21 article-title: Actin protects mammalian eggs against chromosome segregation errors publication-title: Science doi: 10.1126/science.aal1647 contributor: fullname: Schuh – volume: 122 start-page: 2980 year: 2009 end-page: 2988 ident: CR2 article-title: Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity publication-title: J. Cell Sci. doi: 10.1242/jcs.041970 contributor: fullname: Chaponnier – volume: 21 start-page: 410 year: 2011 end-page: 419 ident: CR17 article-title: And the dead shall rise: actin and myosin return to the spindle publication-title: Dev. Cell doi: 10.1016/j.devcel.2011.07.018 contributor: fullname: Bement – volume: 94 start-page: 275 year: 2002 end-page: 288 ident: CR18 article-title: Centrosome separation: respective role of microtubules and actin filaments publication-title: Biol. Cell doi: 10.1016/s0248-4900(02)01202-9 contributor: fullname: Prigent – volume: 12 start-page: 309 year: 2007 end-page: 317 ident: CR31 article-title: Rac activity is polarized and regulates meiotic spindle stability and anchoring in mammalian oocytes publication-title: Dev. Cell doi: 10.1016/j.devcel.2006.12.010 contributor: fullname: Carroll – volume: 235 start-page: 1 year: 2017 end-page: 37 ident: CR1 article-title: Mammalian actins: isoform-specific functions and diseases publication-title: Handb. Exp. Pharmacol. doi: 10.1007/164_2016_43 contributor: fullname: Van Troys – volume: 7 start-page: 72699 year: 2016 end-page: 72715 ident: CR3 article-title: Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells publication-title: Oncotarget doi: 10.18632/oncotarget.12236 contributor: fullname: Kopnin – volume: 14 start-page: 3908 year: 2015 end-page: 3919 ident: CR15 article-title: Gamma-actin is involved in regulating centrosome function and mitotic progression in cancer cells publication-title: Cell Cycle doi: 10.1080/15384101.2015.1120920 contributor: fullname: Kavallaris – volume: 355 start-page: 965 year: 2000 end-page: 970 ident: CR30 article-title: Rho GTPases: molecular switches that control the organization and dynamics of the actin cytoskeleton publication-title: Philos. Trans. R. Soc. Lond. B Biol. Sci. doi: 10.1098/rstb.2000.0632 contributor: fullname: Nobes – volume: 213 start-page: 641 year: 2016 end-page: 649 ident: CR27 article-title: Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage publication-title: J. Cell Biol. doi: 10.1083/jcb.201603025 contributor: fullname: Glotzer – volume: 279 start-page: 35557 year: 2004 end-page: 35563 ident: CR6 article-title: Mechanism of blebbistatin inhibition of myosin II publication-title: J. Biol. Chem. doi: 10.1074/jbc.M405319200 contributor: fullname: Sellers – volume: 25 start-page: 270 year: 2013 end-page: 283 ident: CR19 article-title: Mitotic rounding alters cell geometry to ensure efficient bipolar spindle formation publication-title: Dev. Cell doi: 10.1016/j.devcel.2013.03.014 contributor: fullname: Baum – volume: 28 start-page: 983 year: 2006 end-page: 993 ident: CR10 article-title: Rho GTPase activity zones and transient contractile arrays publication-title: BioEssays doi: 10.1002/bies.20477 contributor: fullname: Von Dassow – volume: 11 start-page: 39 year: 1995 end-page: 48 ident: CR28 article-title: Translocation of activated Rho from the cytoplasm to membrane ruffling area, cell–cell adhesion sites and cleavage furrows publication-title: Oncogene contributor: fullname: Takai – volume: 117 start-page: 361 year: 2004 end-page: 372 ident: CR20 article-title: Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assembly publication-title: Cell doi: 10.1016/S0092-8674(04)00341-1 contributor: fullname: McGee – volume: 106 start-page: 761 year: 1988 end-page: 771 ident: CR5 article-title: Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line publication-title: J. Cell Biol. doi: 10.1083/jcb.106.3.761 contributor: fullname: Fusenig – volume: 420 start-page: 629 year: 2002 end-page: 635 ident: CR9 article-title: Rho GTPases in cell biology publication-title: Nature doi: 10.1038/nature01148 contributor: fullname: Hall – volume: 10 start-page: a018267 year: 2018 ident: CR24 article-title: The actin cytoskeleton and actin-based motility publication-title: Cold Spring Harb. Perspect. Biol. doi: 10.1101/cshperspect.a018267 contributor: fullname: Svitkina – ident: CR7 – volume: 6 start-page: 14556 year: 2015 end-page: 14571 ident: CR32 article-title: Tumor promotion by γ and suppression by β non-muscle actin isoforms publication-title: Oncotarget doi: 10.18632/oncotarget.3989 contributor: fullname: Kopnin – volume: 70 start-page: 389 year: 1992 end-page: 399 ident: CR26 article-title: The small GTP-binding protein Rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors publication-title: Cell doi: 10.1016/0092-8674(92)90163-7 contributor: fullname: Hall – volume: 357 start-page: eaal1647 year: 2017 ident: 2063_CR21 publication-title: Science doi: 10.1126/science.aal1647 contributor: fullname: B. Mogessie – volume: 299 start-page: 1743 year: 2003 ident: 2063_CR8 publication-title: Science doi: 10.1126/science.1081412 contributor: fullname: A. F. Straight – volume: 420 start-page: 629 year: 2002 ident: 2063_CR9 publication-title: Nature doi: 10.1038/nature01148 contributor: fullname: S. Etienne-Manneville – volume: 20 start-page: 38 year: 2019 ident: 2063_CR4 publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/s41580-018-0067-1 contributor: fullname: M. Dogterom – volume: 10 start-page: a018267 year: 2018 ident: 2063_CR24 publication-title: Cold Spring Harb. Perspect. Biol. doi: 10.1101/cshperspect.a018267 contributor: fullname: T. Svitkina – volume: 14 start-page: 3908 year: 2015 ident: 2063_CR15 publication-title: Cell Cycle doi: 10.1080/15384101.2015.1120920 contributor: fullname: S. T. Po’uha – volume: 12 start-page: 309 year: 2007 ident: 2063_CR31 publication-title: Dev. Cell doi: 10.1016/j.devcel.2006.12.010 contributor: fullname: G. Halet – volume: 117 start-page: 361 year: 2004 ident: 2063_CR20 publication-title: Cell doi: 10.1016/S0092-8674(04)00341-1 contributor: fullname: J. Rosenblatt – volume: 94 start-page: 275 year: 2002 ident: 2063_CR18 publication-title: Biol. Cell doi: 10.1016/s0248-4900(02)01202-9 contributor: fullname: R. Uzbekov – volume: 6 start-page: 14556 year: 2015 ident: 2063_CR32 publication-title: Oncotarget doi: 10.18632/oncotarget.3989 contributor: fullname: V. Dugina – volume: 279 start-page: 35557 year: 2004 ident: 2063_CR6 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M405319200 contributor: fullname: M. Kovács – volume: 69 start-page: 919 year: 2012 ident: 2063_CR22 publication-title: Cytoskeleton (Hoboken) doi: 10.1002/cm.21071 contributor: fullname: S. N. Jordan – volume: 355 start-page: 965 year: 2000 ident: 2063_CR30 publication-title: Philos. Trans. R. Soc. Lond. B Biol. Sci. doi: 10.1098/rstb.2000.0632 contributor: fullname: A. Hall – volume: 85 start-page: 1025 year: 2011 ident: 2063_CR14 publication-title: Biol. Reprod. doi: 10.1095/biolreprod.111.091736 contributor: fullname: C. Brockmann – volume: 25 start-page: 270 year: 2013 ident: 2063_CR19 publication-title: Dev. Cell doi: 10.1016/j.devcel.2013.03.014 contributor: fullname: O. Lancaster – volume: 103 start-page: 10166 year: 2006 ident: 2063_CR11 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.0603704103 contributor: fullname: H. Xiao – volume: 11 start-page: 39 year: 1995 ident: 2063_CR28 publication-title: Oncogene contributor: fullname: K. Takaishi – volume: 21 start-page: 410 year: 2011 ident: 2063_CR17 publication-title: Dev. Cell doi: 10.1016/j.devcel.2011.07.018 contributor: fullname: J. C. Sandquist – volume: 30 start-page: 1477 year: 2020 ident: 2063_CR23 publication-title: Curr. Biol. doi: 10.1016/j.cub.2020.02.023 contributor: fullname: X. Chen – volume: 106 start-page: 761 year: 1988 ident: 2063_CR5 publication-title: J. Cell Biol. doi: 10.1083/jcb.106.3.761 contributor: fullname: P. Boukamp – ident: 2063_CR7 doi: 10.1201/9780203833445 – volume: 28 start-page: 983 year: 2006 ident: 2063_CR10 publication-title: BioEssays doi: 10.1002/bies.20477 contributor: fullname: W. M. Bement – volume: 8 start-page: 1530 year: 2017 ident: 2063_CR16 publication-title: Nat. Commun. doi: 10.1038/s41467-017-01231-x contributor: fullname: A. Chen – volume: 70 start-page: 389 year: 1992 ident: 2063_CR26 publication-title: Cell doi: 10.1016/0092-8674(92)90163-7 contributor: fullname: A. J. Ridley – volume: 122 start-page: 2980 year: 2009 ident: 2063_CR2 publication-title: J. Cell Sci. doi: 10.1242/jcs.041970 contributor: fullname: V. Dugina – volume: 18 start-page: 30 year: 2008 ident: 2063_CR25 publication-title: Curr. Biol. doi: 10.1016/j.cub.2007.11.068 contributor: fullname: A. J. Piekny – volume: 19 start-page: 6059 year: 2000 ident: 2063_CR29 publication-title: Oncogene doi: 10.1038/sj.onc.1203987 contributor: fullname: H. Kosako – volume: 34 start-page: 525 year: 1967 ident: 2063_CR13 publication-title: J. Cell Biol. doi: 10.1083/jcb.34.2.525 contributor: fullname: G. G. Borisy – volume: 34 start-page: 535 year: 1967 ident: 2063_CR12 publication-title: J. Cell Biol. doi: 10.1083/jcb.34.2.535 contributor: fullname: G. G. Borisy – volume: 213 start-page: 641 year: 2016 ident: 2063_CR27 publication-title: J. Cell Biol. doi: 10.1083/jcb.201603025 contributor: fullname: E. Wagner – volume: 17 start-page: 2610 year: 2018 ident: 2063_CR33 publication-title: Cell Cycle doi: 10.1080/15384101.2018.1553337 contributor: fullname: V. Dugina – volume: 7 start-page: 72699 year: 2016 ident: 2063_CR3 publication-title: Oncotarget doi: 10.18632/oncotarget.12236 contributor: fullname: V. Dugina – volume: 235 start-page: 1 year: 2017 ident: 2063_CR1 publication-title: Handb. Exp. Pharmacol. doi: 10.1007/164_2016_43 contributor: fullname: C. Ampe |
| SSID | ssj0002093 |
| Score | 2.3748071 |
| Snippet | We investigated distribution and functions of beta- and gamma-cytoplasmic actins (CYAs) at different stages of non-neoplastic epithelial cell division using... |
| SourceID | proquest gale crossref pubmed springer |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 1072 |
| SubjectTerms | Actin Actins - metabolism Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Cells, Cultured Comparative analysis Cytokinesis Cytoplasm - metabolism Humans Keratinocytes - cytology Keratinocytes - metabolism Life Sciences Microbiology Microtubules - metabolism Mitosis Muscle proteins Protein Isoforms RNA Tubulins |
| Title | Divergent Impact of Actin Isoforms on Division of Epithelial Cells |
| URI | https://link.springer.com/article/10.1134/S0006297920090072 https://www.ncbi.nlm.nih.gov/pubmed/33050852 https://search.proquest.com/docview/2451130909 |
| Volume | 85 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwEB71IQSXFloKC2VlJAQSKKvE8SbxMdnu0oKoEGKl5RQ5jnPpklRN9lB-PTNOsur2cegliWQndvyY-cae-QzwITMF4gjBHRVK7ojc9xwltXTcPBJCGW24oNjhH-fB6Vx8W4wXW8DXSxflxajfkbSCuj12RFBIrxtwiV9EVEB819uwi6qHJuNu_PXP9-la_nK3o9pFW5le6PYy7_3Ihja6LZNvKKVbu6RW-cz224DA2nIWks_JxWjVZCP97y6j4yP-6znsdViUxe3geQFbpjyAw7hEO_zvNfvIrHeoXXY_gCdJ__R00p8RdwjJCbl1UHQWO7PhlqwqWIwitGRndUV4uGZVyTCXDWGn1OklRYEscdiziVku65cwn01_T06d7lQGR4swbByELLIgFjNPKhOoUAvuqszNLVObCoNAa52hHVVk7tgUBR_nrtF-oDwTcREiejiCnbIqzWtg1FcEKXyP-NJ1JN1AhkVk0C7MicluAJ_73kkvW_KN1BotvkjvtNsAPlH_pTQxmyulVRdfgEURxVUaB8S8I8c8GsDxRk5sM72R_L4fASklkRdaaapVnXIic_OxODmAV-3QWNfLR8GJ8BWr8aXv57QTB_XDlX7zqNxv4Rknc9-6uB3DTnO1Mu8QEzXZELbDRYjXaOINcUIkJ8ls2E0MvCfT85-_MHXO4__j3f_O |
| link.rule.ids | 314,780,784,27924,27925,31720,33267,33745,41081,41523,42150,42592,52111,52234 |
| linkProvider | Springer Nature |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LT8MwDLZ4CMEF8WYwIEgIJFClLs3a5lgGaON1YhK3KE3T02gRHQf-PXbWTozHgVulWElkJ3409meAk9Tm6EcI7ulIck9kQcfT0kjPz2IhtDWWC6odfngM-0Nx-9x9ruu4qybbvXmSdJp60ndEUE2vH3KJU6JbQIDX87CIB05S24IhT6bql_s10i6GykReP2X-OsWMMfqukr_YpG-PpM723KzBau00smQi5XWYs8UGbCYFBswvH-yUuTRO9398A5Yum6_lXtPMbRMuryj_gsqo2MDVRbIyZwnquoINqpIc14qVBUMqV2tOo9evVK4xwvPJenY0qrZgeHP91Ot7dfsEz4goGnvoW8ic4MY6UttQR0ZwX6d-5iDVdBSGxpgUA5489bs2z3k3860JQt2xMRcRmvltWCjKwu4CI66S7Q86BGxuYumHMspjiwFcRpBzLThv-KheJygZykUXgVA_mN6CM-K0ohs0ftNG14UAuBRhUakkJIgc2eVxC9ozlMgzMzN83MhK0RClixW2fK8UJ9S1AJeTLdiZCHG6rwA1HPqZuI2LRqqqvrfV35ve-xf1ESz3nx7u1f3g8W4fVjjF6C4vrQ0L47d3e4COzDg9dAf3E-tg4Uw |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3dT9swED8xEIMXxNoNCoV5EtqkTRGp4ybxYyhUdBsVD6vEm-U4zlNJKhIe-O-5cxNEgT3wFskn27qz7yO--x3ASWpz9CME93QkuSeyYOBpaaTnZ7EQ2hrLBdUOX03Dy5n4fTO8afqcVm22e_skuaxpIJSmoj5dZHnTg0RQfa8fconTo4tA4NcfYIMsER30GU-eVDH3G9RdDJuJvHnWfHOKFcP0Uj0_s08vHkydHRrvwk7jQLJkKfFPsGaLDnSTAoPn2wf2nbmUTvevvAObZ-3X1qht7NaFs3PKxaCSKjZxNZKszFmCeq9gk6okJ7ZiZcGQytWd0-jFgko35nhW2cjO59VnmI0v_o0uvaaVgmdEFNUe-hkyJ-ixgdQ21JER3Nepnzl4NR2FoTEmxeAnT_2hzXM-zHxrglAPbMxFhCb_C6wXZWH3gRFXyQ8IBgRybmLphzLKY4vBXEbwcz342fJRLZaIGcpFGoFQr5jegx_EaUW3qb7TRjdFAbgU4VKpJCS4HDnkcQ_6K5TIM7My_K2VlaIhSh0rbHlfKU4IbAEuJ3uwtxTi074C1Hboc-I2frVSVc0drv6_6YN3UX-Fj9fnY_V3Mv1zCNucwnWXotaH9fru3h6hT1Onx-7cPgJl5OWI |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Divergent+Impact+of+Actin+Isoforms+on+Division+of+Epithelial+Cells&rft.jtitle=Biochemistry+%28Moscow%29&rft.au=Shagieva%2C+G.+S&rft.au=Alieva%2C+I.+B&rft.au=Chaponnier%2C+C&rft.au=Dugina%2C+V.+B&rft.date=2020-09-01&rft.pub=Springer&rft.issn=0006-2979&rft.volume=85&rft.issue=9&rft.spage=1072&rft_id=info:doi/10.1134%2FS0006297920090072&rft.externalDocID=A636269528 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-2979&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-2979&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-2979&client=summon |