HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay

• Published in: Chemical science (Cambridge) Vol. 7; no. 3; pp. 2094 - 2099
• Main Authors: Hu, Jun Jacob, Wong, Nai-Kei, Lu, Ming-Yang, Chen, Xingmiao, Ye, Sen, Zhao, Angela Qian, Gao, Peng, Yi-Tsun Kao, Richard, Shen, Jiangang, Yang, Dan
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 01.03.2016; Royal Society of Chemistry
• Subjects: Assaying; Biology; Chemistry; Chemistry, Multidisciplinary; Embryos; Flow cytometry; Fluorescence; Laboratory apparatus; Physical Sciences; Robustness; Science & Technology; Zebrafish; OXIDATION; HOCL; MYELOPEROXIDASE; REACTIVE OXYGEN; PEROXYNITRITE; MITOCHONDRIA; BIOLOGY; NEURODEGENERATION; SELECTIVE DETECTION

Ultra-selective and ultra-sensitive probes for hypochlorous acid (HOCl), one of the most poorly understood reactive oxygen species (ROS), are urgently needed to unravel the HOCl functions in important biological processes such as development and innate immunity. Based on a selective oxidative O -dea...