Comparative glycoproteomics based on lectins affinity capture of N-linked glycoproteins from human Chang liver cells and MHCC97-H cells

• Published in: Proteomics (Weinheim) Vol. 7; no. 14; pp. 2358 - 2370
• Main Authors: Xu, Zhibin, Zhou, Xinwen, Lu, Haojie, Wu, Nan, Zhao, Hongbo, Zhang, Lineng, Zhang, Wen, Liang, Yu Long, Wang, Liying, Liu, Yinkun, Yang, Pengyuan, Zha, Xiliang
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.07.2007; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cells, Cultured; Chromatography, Affinity - methods; Concanavalin A - metabolism; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Glycoprotein; Glycoproteins - analysis; Glycoproteins - chemistry; Glycoproteins - metabolism; Glycoproteomics; Hepatocytes - chemistry; Hepatocytes - metabolism; Humans; Lectin; Liver; Miscellaneous; Proteins; Proteomics - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Up-Regulation; Wheat Germ Agglutinins - metabolism; Human; Lectin; Glycoproteomics; Liver; Glycoprotein; Proteomics

We present here an effective technique for the large‐scale separation and identification of N‐linked glycoproteins from Chang liver cells, the human normal liver cells. To enrich N‐linked glycoproteins from the whole cells, a procedure containing the lysis of human liver cells, the solubilization of...