Microarray analysis of isolated human islet transcriptome in type 2 diabetes and the role of the ubiquitin–proteasome system in pancreatic beta cell dysfunction

• Published in: Molecular and cellular endocrinology Vol. 367; no. 1-2; pp. 1 - 10
• Main Authors: Bugliani, Marco, Liechti, Robin, Cheon, Hwanju, Suleiman, Mara, Marselli, Lorella, Kirkpatrick, Clare, Filipponi, Franco, Boggi, Ugo, Xenarios, Ioannis, Syed, Farooq, Ladriere, Laurence, Wollheim, Claes, Lee, Myung-Shik, Marchetti, Piero
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 10.03.2013
• Subjects: Aged; Animals; Beta cells; Cell Line; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - pathology; Diabetes Mellitus, Type 2 - physiopathology; Female; gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation - drug effects; genes; Humans; Immunohistochemistry; Insulin - metabolism; Insulin Secretion; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - metabolism; Insulin-Secreting Cells - pathology; Lipotoxicity; Male; Microarray; microarray technology; Middle Aged; noninsulin-dependent diabetes mellitus; Oligonucleotide Array Sequence Analysis; Palmitates - pharmacology; phenotype; proteasome endopeptidase complex; Proteasome Endopeptidase Complex - genetics; Proteasome Endopeptidase Complex - metabolism; Proteasome Inhibitors - pharmacology; protein degradation; Rats; Reverse Transcriptase Polymerase Chain Reaction; transcriptome; Transcriptome - genetics; Type 2 diabetes; ubiquitin; Ubiquitin - genetics; Ubiquitin - metabolism; Ubiquitinated Proteins - genetics; Ubiquitinated Proteins - metabolism; Ubiquitin–proteasome system; Beta cells; Type 2 diabetes; Microarray; Lipotoxicity; Ubiquitin–proteasome system

► Islets from type 2 diabetic subjects have several transcriptome alterations. ► The ubiquitin–proteasome system (UPS) is deranged in human type 2 diabetic islets. ► Environmental factors can affect islet UPS. To shed light on islet cell molecular phenotype in human type 2 diabetes (T2D), we studied...