In vivo and in vitro expression of the plasminogen activators and urokinase type plasminogen activator receptor (u-PAR) in the pig oviduct

• Published in: Animal reproduction science Vol. 136; no. 1-2; pp. 90 - 99
• Main Authors: Roldán-Olarte, Mariela, García, Daniela C, Jiménez-Díaz, María, Valdecantos, Pablo A, Miceli, Dora C
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2012
• Subjects: Animals; blood plasma; cell culture; epithelial cells; Estradiol - pharmacology; estrous cycle; Estrous Cycle - physiology; Female; gene expression; genes; immunohistochemistry; messenger RNA; Oviduct; Oviducts - drug effects; Oviducts - enzymology; Oviducts - metabolism; ovulation; Pig; plasmin; plasminogen; Plasminogen Activator Inhibitor 1 - genetics; Plasminogen Activator Inhibitor 1 - metabolism; Plasminogen activators; Porcine; progesterone; Progesterone - pharmacology; protein synthesis; Receptors, Urokinase Plasminogen Activator - genetics; Receptors, Urokinase Plasminogen Activator - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; swine; Swine - physiology; Tissue Plasminogen Activator - genetics; Tissue Plasminogen Activator - metabolism; transcription (genetics); u-PAR; u-plasminogen activator; Urokinase-Type Plasminogen Activator - genetics; Urokinase-Type Plasminogen Activator - metabolism; Porcine; Plasminogen activators; Oviduct; u-PAR; Pig
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2012.09.013

Plasminogen activator activities have previously been reported in oviductal fluid. At present the question was whether the source of these activities is molecules come from blood plasma or if these activators are synthesized by the oviduct. Gene expression and protein synthesis of urokinase type (u-PA) and tissue type (t-PA) occur in different regions of the pig oviduct. Their relative concentrations do not vary between the ampulla and isthmus regions and are similar throughout the estrous cycle. However, while relative amounts of t-PA mRNA were not different between the different stages of the estrous cycle, u-PA mRNA was greater after ovulation (P<0.05). Regarding the function of u-PA, its receptor (u-PAR) was distinguished by immunohistochemistry at the apical region of the epithelial cells and was more noticeable in the isthmus. Expression of u-PA, t-PA, u-PAR and PAI-1 genes in primary oviductal epithelial cell cultures was studied under 17-β-estradiol (100pg/ml) and progesterone (100ng/ml). u-PA mRNA increased in the presence of progesterone (P<0.05), but not by action of 17-β-estradiol. t-PA, PAI-1 and u-PAR were similar when cultured with the hormones. These results suggest that u-PA could be regulated by progesterone at a transcriptional level, by the balance of their activity for PAI-1 or at the epithelial surface through the binding of u-PAR. In conclusion, plasminogen activation system components might cooperate in the oviductal lumen to control plasmin generation.