Histone H3 Lysine 27 demethylases Jmjd3 and Utx are required for T-cell differentiation

• Published in: Nature communications Vol. 6; no. 1; p. 8152
• Main Authors: Manna, Sugata, Kim, Jong Kyong, Baugé, Catherine, Cam, Margaret, Zhao, Yongmei, Shetty, Jyoti, Vacchio, Melanie S., Castro, Ehydel, Tran, Bao, Tessarollo, Lino, Bosselut, Rémy
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.09.2015; Nature Publishing Group; Nature Pub. Group
• Subjects: 38/15; 38/61; 42/41; 631/250/1619/554; 631/250/2152/1566/2493; 631/80/86; 64/60; 96/1; Animals; Cancer research; CD4-Positive T-Lymphocytes - cytology; CD4-Positive T-Lymphocytes - metabolism; Cell Differentiation; Cell division; Chromatin Immunoprecipitation; DNA methylation; Enzymes; Flow Cytometry; Gene expression; Histone Demethylases - genetics; Humanities and Social Sciences; In Vitro Techniques; Jumonji Domain-Containing Histone Demethylases - genetics; Life Sciences; Medical research; Mice; Mice, Inbred C57BL; Mice, Knockout; multidisciplinary; Real-Time Polymerase Chain Reaction; Receptors, Lysosphingolipid - metabolism; Science; Science (multidisciplinary); Stem cells; Thymocytes - cytology; Thymocytes - metabolism; Transcription factors; United States > US; Maryland

Although histone H3 lysine 27 trimethylation (H3K27Me3) is associated with gene silencing, whether H3K27Me3 demethylation affects transcription and cell differentiation in vivo has remained elusive. To investigate this, we conditionally inactivated the two H3K27Me3 demethylases, Jmjd3 and Utx, in no...